6h7g - Revision history

OCA at 06:50, 17 April 2019

OCA — Wed, 17 Apr 2019 06:50:36 GMT

←Older revision		Revision as of 06:50, 17 April 2019
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	<StructureSection load='6h7g' size='340' side='right'caption='[[6h7g]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='6h7g' size='340' side='right'caption='[[6h7g]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h7g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H7G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H7G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoribulokinase Phosphoribulokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.19 2.7.1.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoribulokinase Phosphoribulokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.19 2.7.1.19] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h7g OCA], [http://pdbe.org/6h7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h7g RCSB], [http://www.ebi.ac.uk/pdbsum/6h7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h7g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h7g OCA], [http://pdbe.org/6h7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h7g RCSB], [http://www.ebi.ac.uk/pdbsum/6h7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h7g ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlre]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Phosphoribulokinase]]		[[Category: Phosphoribulokinase]]

OCA at 07:19, 10 April 2019

OCA — Wed, 10 Apr 2019 07:19:54 GMT

←Older revision		Revision as of 07:19, 10 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h7g is ~~ON HOLD~~	+	==Crystal structure of redox-sensitive phosphoribulokinase (PRK) from the green algae Chlamydomonas reinhardtii==
		+	<StructureSection load='6h7g' size='340' side='right'caption='[[6h7g]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h7g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H7G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoribulokinase Phosphoribulokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.19 2.7.1.19] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h7g OCA], [http://pdbe.org/6h7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h7g RCSB], [http://www.ebi.ac.uk/pdbsum/6h7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h7g ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In land plants and algae, the Calvin-Benson (CB) cycle takes place in the chloroplast, a specialized organelle in which photosynthesis occurs. Thioredoxins (TRXs) are small ubiquitous proteins, known to harmonize the two stages of photosynthesis through a thiol-based mechanism. Among the 11 enzymes of the CB cycle, the TRX target phosphoribulokinase (PRK) has yet to be characterized at the atomic scale. To accomplish this goal, we determined the crystal structures of PRK from two model species: the green alga Chlamydomonas reinhardtii (CrPRK) and the land plant Arabidopsis thaliana (AtPRK). PRK is an elongated homodimer characterized by a large central beta-sheet of 18 strands, extending between two catalytic sites positioned at its edges. The electrostatic surface potential of the catalytic cavity has both a positive region suitable for binding the phosphate groups of substrates and an exposed negative region to attract positively charged TRX-f. In the catalytic cavity, the regulatory cysteines are 13 A apart and connected by a flexible region exclusive to photosynthetic eukaryotes-the clamp loop-which is believed to be essential for oxidation-induced structural rearrangements. Structural comparisons with prokaryotic and evolutionarily older PRKs revealed that both AtPRK and CrPRK have a strongly reduced dimer interface and an increased number of random-coiled regions, suggesting that a general loss in structural rigidity correlates with gains in TRX sensitivity during the molecular evolution of PRKs in eukaryotes.

-	~~Authors: Fermani, S., Sparla, F~~., Gurrieri, L., Demitri, N., ~~Polentarutti~~, M., ~~Falini~~, G., ~~Trost~~, P., Lemaire, S.D.	+	Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle.,Gurrieri L, Del Giudice A, Demitri N, Falini G, Pavel NV, Zaffagnini M, Polentarutti M, Crozet P, Marchand CH, Henri J, Trost P, Lemaire SD, Sparla F, Fermani S Proc Natl Acad Sci U S A. 2019 Mar 28. pii: 1820639116. doi:, 10.1073/pnas.1820639116. PMID:30923119<ref>PMID:30923119</ref>

-	~~Description: Crystal structure~~ of ~~redox-sensitive phosphoribulokinase (PRK) from~~ the ~~green algae Chlamydomonas reinhardtii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Gurrieri, L]]~~	+	<div class="pdbe-citations 6h7g" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Sparla, F~~]]	+	== References ==
-	[[Category: ~~Trost, P~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Phosphoribulokinase]]
	[[Category: Demitri, N]]		[[Category: Demitri, N]]
	[[Category: Falini, G]]		[[Category: Falini, G]]
-	[[Category: Lemaire, S.D]]
-	[[Category: Polentarutti, M]]
	[[Category: Fermani, S]]		[[Category: Fermani, S]]
		+	[[Category: Gurrieri, L]]
		+	[[Category: Lemaire, S D]]
		+	[[Category: Polentarutti, M]]
		+	[[Category: Sparla, F]]
		+	[[Category: Trost, P]]
		+	[[Category: Atp binding]]
		+	[[Category: Kinase activity]]
		+	[[Category: Photosynthesis]]
		+	[[Category: Transferase]]

OCA: Protected "6h7g" [edit=sysop:move=sysop]

OCA — Thu, 09 Aug 2018 20:07:26 GMT

Protected "6h7g" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:07, 9 August 2018

OCA: New page: '''Unreleased structure''' The entry 6h7g is ON HOLD Authors: Fermani, S., Sparla, F., Gurrieri, L., Demitri, N., Polentarutti, M., Falini, G., Trost, P., Lemaire, S.D. Description: Cr...

OCA — Thu, 09 Aug 2018 20:07:24 GMT

New page: '''Unreleased structure''' The entry 6h7g is ON HOLD Authors: Fermani, S., Sparla, F., Gurrieri, L., Demitri, N., Polentarutti, M., Falini, G., Trost, P., Lemaire, S.D. Description: Cr...

New page

'''Unreleased structure'''

The entry 6h7g is ON HOLD

Authors: Fermani, S., Sparla, F., Gurrieri, L., Demitri, N., Polentarutti, M., Falini, G., Trost, P., Lemaire, S.D.

Description: Crystal structure of redox-sensitive phosphoribulokinase (PRK) from the green algae Chlamydomonas reinhardtii
[[Category: Unreleased Structures]]
[[Category: Gurrieri, L]]
[[Category: Sparla, F]]
[[Category: Trost, P]]
[[Category: Demitri, N]]
[[Category: Falini, G]]
[[Category: Lemaire, S.D]]
[[Category: Polentarutti, M]]
[[Category: Fermani, S]]