6ha0 - Revision history

OCA at 06:47, 3 July 2019

OCA — Wed, 03 Jul 2019 06:47:35 GMT

←Older revision		Revision as of 06:47, 3 July 2019
Line 3:		Line 3:
	<StructureSection load='6ha0' size='340' side='right'caption='[[6ha0]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6ha0' size='340' side='right'caption='[[6ha0]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ha0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ha0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Group_a_rotaviruses Group a rotaviruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HA0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28875 Group A rotaviruses])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ha0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ha0 OCA], [http://pdbe.org/6ha0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ha0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ha0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ha0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ha0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ha0 OCA], [http://pdbe.org/6ha0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ha0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ha0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ha0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rotavirus is the leading agent causing acute gastroenteritis in young children, with the P[8] genotype accounting for more than 80% of infections in humans. The molecular bases for binding of the VP8* domain from P[8] VP4 spike protein to its cellular receptor, the secretory H type-1 antigen (Fuc-alpha1,2-Gal-beta1,3-GlcNAc; H1), and to its precursor lacto-N-biose (Gal-beta1,3-GlcNAc; LNB) have been determined. The resolution of P[8] VP8* crystal structures in complex with H1 antigen and LNB and site-directed mutagenesis experiments revealed that both glycans bind to the P[8] VP8* protein through a binding pocket shared with other members of the P[II] genogroup (i.e.: P[4], P[6] and P[19]). Our results show that the L-fucose moiety from H1 only displays indirect contacts with P[8] VP8. However, the induced conformational changes in the LNB moiety increase the ligand affinity by two-fold, as measured by surface plasmon resonance (SPR), providing a molecular explanation for the different susceptibility to rotavirus infection between secretor and non-secretor individuals. The unexpected interaction of P[8] VP8 with LNB, a building block of type-1 human milk oligosaccharides, resulted in inhibition of rotavirus infection, highlighting the role and possible application of this disaccharide as an antiviral. While key amino acids in the H1/LNB binding pocket were highly conserved in members of the P[II] genogroup, differences were found in ligand affinities among distinct P[8] genetic lineages. The variation in affinities were explained by subtle structural differences induced by amino acid changes in the vicinity of the binding pocket, providing a fine-tuning mechanism for glycan binding in P[8] rotavirus.
		+
		+	Unraveling the role of the secretor antigen in human rotavirus attachment to histo-blood group antigens.,Gozalbo-Rovira R, Ciges-Tomas JR, Vila-Vicent S, Buesa J, Santiso-Bellon C, Monedero V, Yebra MJ, Marina A, Rodriguez-Diaz J PLoS Pathog. 2019 Jun 21;15(6):e1007865. doi: 10.1371/journal.ppat.1007865. PMID:31226167<ref>PMID:31226167</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ha0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Group a rotaviruses]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Buesa, J]]		[[Category: Buesa, J]]

OCA at 05:33, 12 June 2019

OCA — Wed, 12 Jun 2019 05:33:29 GMT

←Older revision		Revision as of 05:33, 12 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ha0 ~~is ON HOLD until Paper Publication~~	+	==Unraveling the role of the secretor antigen in human rotavirus attachment to histo-blood group antigens==
-		+	<StructureSection load='6ha0' size='340' side='right'caption='[[6ha0]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~Authors~~: ~~Ciges~~-~~Tomas, J~~.R.~~, Gozalbo~~-~~Rovira, R~~., ~~Vila~~-~~Vicent~~, S.~~, Buesa, J~~.~~, Santiso~~-~~Bellon~~, C., ~~Monedero~~, V., ~~Yebra, M~~.J., ~~Rodriguez~~-~~Diaz~~, J., ~~Marina, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ha0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HA0 FirstGlance]. <br>
-	~~Description~~: ~~Molecular bases of histo~~-~~blood group antigen recognition by the most common human rotavirus~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ha0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ha0 OCA], [http://pdbe.org/6ha0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ha0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ha0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ha0 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Buesa, J]]
		+	[[Category: Ciges-Tomas, J R]]
		+	[[Category: Gozalbo-Rovira, R]]
		+	[[Category: Marina, A]]
	[[Category: Monedero, V]]		[[Category: Monedero, V]]
-	[[Category: Ciges-Tomas, J.R]]
-	[[Category: Buesa, J]]
	[[Category: Rodriguez-Diaz, J]]		[[Category: Rodriguez-Diaz, J]]
	[[Category: Santiso-Bellon, C]]		[[Category: Santiso-Bellon, C]]
-	[[Category: Yebra, M.J]]
	[[Category: Vila-Vicent, S]]		[[Category: Vila-Vicent, S]]
-	[[Category: ~~Marina~~, A]]	+	[[Category: Yebra, M J]]
-	[[Category: ~~Gozalbo~~-~~Rovira, R~~]]	+	[[Category: Histo-blood group antigen rotavirus]]
		+	[[Category: Viral protein]]

OCA: Protected "6ha0" [edit=sysop:move=sysop]

OCA — Wed, 15 Aug 2018 15:01:26 GMT

Protected "6ha0" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:01, 15 August 2018

OCA: New page: '''Unreleased structure''' The entry 6ha0 is ON HOLD until Paper Publication Authors: Ciges-Tomas, J.R., Gozalbo-Rovira, R., Vila-Vicent, S., Buesa, J., Santiso-Bellon, C., Monedero, V....

OCA — Wed, 15 Aug 2018 15:01:24 GMT

New page: '''Unreleased structure''' The entry 6ha0 is ON HOLD until Paper Publication Authors: Ciges-Tomas, J.R., Gozalbo-Rovira, R., Vila-Vicent, S., Buesa, J., Santiso-Bellon, C., Monedero, V....

New page

'''Unreleased structure'''

The entry 6ha0 is ON HOLD until Paper Publication

Authors: Ciges-Tomas, J.R., Gozalbo-Rovira, R., Vila-Vicent, S., Buesa, J., Santiso-Bellon, C., Monedero, V., Yebra, M.J., Rodriguez-Diaz, J., Marina, A.

Description: Molecular bases of histo-blood group antigen recognition by the most common human rotavirus
[[Category: Unreleased Structures]]
[[Category: Monedero, V]]
[[Category: Ciges-Tomas, J.R]]
[[Category: Buesa, J]]
[[Category: Rodriguez-Diaz, J]]
[[Category: Santiso-Bellon, C]]
[[Category: Yebra, M.J]]
[[Category: Vila-Vicent, S]]
[[Category: Marina, A]]
[[Category: Gozalbo-Rovira, R]]