6hbx - Revision history

OCA at 07:03, 27 March 2019

OCA — Wed, 27 Mar 2019 07:03:57 GMT

←Older revision		Revision as of 07:03, 27 March 2019
Line 1:		Line 1:

	==The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with ethylguanidine==		==The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with ethylguanidine==
-	<StructureSection load='6hbx' size='340' side='right' caption='[[6hbx]], [[Resolution\|resolution]] 1.54Å' scene=''>	+	<StructureSection load='6hbx' size='340' side='right'caption='[[6hbx]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6hbx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HBX FirstGlance]. <br>		<table><tr><td colspan='2'>[[6hbx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HBX FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Huang, L]]		[[Category: Huang, L]]
	[[Category: Lilley, D M.J]]		[[Category: Lilley, D M.J]]

OCA at 06:43, 21 February 2019

OCA — Thu, 21 Feb 2019 06:43:55 GMT

←Older revision		Revision as of 06:43, 21 February 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6hbx is ~~ON HOLD until Paper~~ Publication	+	==The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with ethylguanidine==
		+	<StructureSection load='6hbx' size='340' side='right' caption='[[6hbx]], [[Resolution\|resolution]] 1.54Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hbx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HBX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EGD:N-ETHYLGUANIDINE'>EGD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hbx OCA], [http://pdbe.org/6hbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hbx RCSB], [http://www.ebi.ac.uk/pdbsum/6hbx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hbx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have designed structure-based ligands for the guanidine-II riboswitch that bind with enhanced affinity, exploiting the twin binding sites created by loop-loop interaction. We synthesized diguanidine species, comprising two guanidino groups covalently connected by Cn linkers where n = 4 or 5. Calorimetric and fluorescent analysis shows that these ligands bind with a ten-fold higher affinity to the riboswitch compared to guanidine. We determined X-ray crystal structures of the riboswitch bound to the new ligands, showing that the guanidino groups are bound to both nucleobases and backbone within the binding pockets, analogously to guanidine binding. The connecting chain passes through side openings in the binding pocket and traverses the minor groove of the RNA. The combination of the riboswitch loop-loop interaction and our novel ligands have potential applications in chemical biology.

-	~~Authors~~:	+	Structure-guided design of a high affinity ligand for a riboswitch.,Huang L, Wang J, Wilson TJ, Lilley DMJ RNA. 2019 Jan 4. pii: rna.069567.118. doi: 10.1261/rna.069567.118. PMID:30609994<ref>PMID:30609994</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6hbx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huang, L]]
		+	[[Category: Lilley, D M.J]]
		+	[[Category: Dimer]]
		+	[[Category: Guanidine ii riboswitch]]
		+	[[Category: Rna]]
		+	[[Category: Stem-loop]]
		+	[[Category: Tetra loop]]

OCA: Protected "6hbx" [edit=sysop:move=sysop]

OCA — Wed, 22 Aug 2018 04:55:26 GMT

Protected "6hbx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 22 August 2018

OCA: New page: '''Unreleased structure''' The entry 6hbx is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Aug 2018 04:55:25 GMT

New page: '''Unreleased structure''' The entry 6hbx is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hbx is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]