6hd8 - Revision history

OCA at 07:08, 15 April 2020

OCA — Wed, 15 Apr 2020 07:08:58 GMT

←Older revision		Revision as of 07:08, 15 April 2020
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	<StructureSection load='6hd8' size='340' side='right'caption='[[6hd8]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6hd8' size='340' side='right'caption='[[6hd8]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hd8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HD8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HD8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hd8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Flexibacter_tractuosus Flexibacter tractuosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HD8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HD8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hd8 OCA], [http://pdbe.org/6hd8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hd8 RCSB], [http://www.ebi.ac.uk/pdbsum/6hd8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hd8 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, b4034, JW3994 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama]), Ftrac_2467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=643867 Flexibacter tractuosus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hd8 OCA], [http://pdbe.org/6hd8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hd8 RCSB], [http://www.ebi.ac.uk/pdbsum/6hd8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hd8 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The TMEM175 family constitutes recently discovered K(+) channels that are important for autophagosome turnover and lysosomal pH regulation and are associated with the early onset of Parkinson Disease. TMEM175 channels lack a P-loop selectivity filter, a hallmark of all known K(+) channels, raising the question how selectivity is achieved. Here, we report the X-ray structure of a closed bacterial TMEM175 channel in complex with a nanobody fusion-protein disclosing bound K(+) ions. Our analysis revealed that a highly conserved layer of threonine residues in the pore conveys a basal K(+) selectivity. An additional layer comprising two serines in human TMEM175 increases selectivity further and renders this channel sensitive to 4-aminopyridine and Zn(2+). Our findings suggest that large hydrophobic side chains occlude the pore, forming a physical gate, and that channel opening by iris-like motions simultaneously relocates the gate and exposes the otherwise concealed selectivity filter to the pore lumen.
		+
		+	Structural basis for ion selectivity in TMEM175 K(+) channels.,Brunner JD, Jakob RP, Schulze T, Neldner Y, Moroni A, Thiel G, Maier T, Schenck S Elife. 2020 Apr 8;9. pii: 53683. doi: 10.7554/eLife.53683. PMID:32267231<ref>PMID:32267231</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hd8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Flexibacter tractuosus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brunner, J D]]		[[Category: Brunner, J D]]

OCA at 15:23, 28 August 2019

OCA — Wed, 28 Aug 2019 15:23:07 GMT

←Older revision		Revision as of 15:23, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hd8 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the potassium channel MtTMEM175 in complex with a Nanobody-MBP fusion protein==
-		+	<StructureSection load='6hd8' size='340' side='right'caption='[[6hd8]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hd8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HD8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HD8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hd8 OCA], [http://pdbe.org/6hd8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hd8 RCSB], [http://www.ebi.ac.uk/pdbsum/6hd8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hd8 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Brunner, J D]]
		+	[[Category: Jakob, R P]]
		+	[[Category: Maier, T]]
		+	[[Category: Moroni, A]]
		+	[[Category: Neldner, Y]]
		+	[[Category: Schenck, S]]
		+	[[Category: Schulze, T]]
		+	[[Category: Thiel, G]]
		+	[[Category: Lysosome]]
		+	[[Category: Potassium channel]]
		+	[[Category: Tmem175]]
		+	[[Category: Transport protein]]

OCA at 06:08, 26 September 2018

OCA — Wed, 26 Sep 2018 06:08:04 GMT

←Older revision		Revision as of 06:08, 26 September 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hd8 is ON HOLD	+	The entry 6hd8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6hd8" [edit=sysop:move=sysop]

OCA — Wed, 29 Aug 2018 05:50:35 GMT

Protected "6hd8" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 29 August 2018

OCA: New page: '''Unreleased structure''' The entry 6hd8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Aug 2018 05:50:34 GMT

New page: '''Unreleased structure''' The entry 6hd8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hd8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]