OCA at 15:24, 28 August 2019

OCA — Wed, 28 Aug 2019 15:24:21 GMT

←Older revision		Revision as of 15:24, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hdy is ~~ON HOLD~~	+	==Crystal structure of 2-Hydroxyisobutyryl-CoA Ligase (HCL) in the postadenylation state in complex with S3-HB-AMP==
		+	<StructureSection load='6hdy' size='340' side='right'caption='[[6hdy]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hdy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HDY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HDY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3HL:(3S)-3-HYDROXYBUTANOIC+ACID'>3HL</scene>, <scene name='pdbligand=8LQ:[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]+(3~{S})-3-oxidanylbutanoate'>8LQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hdy OCA], [http://pdbe.org/6hdy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hdy RCSB], [http://www.ebi.ac.uk/pdbsum/6hdy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hdy ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-Hydroxyisobutyric acid (2-HIBA) is a biomarker of adiposity and associated metabolic diseases such as diabetes mellitus. It is also formed in the bacterial degradation pathway of the fuel oxygenate methyl tert-butyl ether (MTBE), requiring thioesterification with CoA prior to isomerization to 3-hydroxybutyryl-CoA by B12-dependent acyl-CoA mutases. Here, we identify the adenylating enzymes superfamily member 2-HIBA-CoA ligase (HCL) in the MTBE-degrading bacterium Aquincola tertiaricarbonis L108 by knockout experiments. To characterize this central enzyme of 2-HIBA metabolism, ligase activity kinetics of purified HCL and its X-ray crystal structures were studied. We analyzed the enzyme in three states, which differ in the orientation of the two enzyme domains. A 154 degrees rotation of the C-terminal domain accompanies the switch from the adenylate- into the thioester-forming state. Furthermore, a third conformation was obtained, which differs by 50 degrees and 130 degrees from the adenylation and thioesterification states, respectively. Phylogenetic and structural analysis reveals that HCL defines a new subgroup within phenylacetate-CoA ligases (PCLs) thus far described to exclusively accept aromatic acyl substrates. In contrast, kinetic characterization clearly demonstrated that HCL catalyzes CoA activation of several aliphatic short-chain carboxylic acids, preferentially 2-HIBA. Compared to the classical PCL representatives PaaK1 and PaaK2 of Burkholderia cenocepacia J2315, the acyl binding pocket of HCL is significantly smaller and more polar, due to unique active-site residues Y164 and S239 forming H-bonds with the OH-group of the acyl substrate moiety. Furthermore, HCL and PaaK topologies illustrate the evolutionary steps leading from a homodimeric to the fused monomeric core fold found in other ligases.

-	~~Authors: Zahn~~, M., ~~Rohwerder~~, T., ~~Strater~~, N.	+	Structures of 2-Hydroxyisobutyric Acid-CoA Ligase Reveal Determinants of Substrate Specificity and Describe a Multi-Conformational Catalytic Cycle.,Zahn M, Kurteva-Yaneva N, Schuster J, Krug U, Georgi T, Muller RH, Rohwerder T, Strater N J Mol Biol. 2019 Jul 12;431(15):2747-2761. doi: 10.1016/j.jmb.2019.05.027. Epub, 2019 May 28. PMID:31145912<ref>PMID:31145912</ref>

-	~~Description: Crystal structure~~ of ~~2-Hydroxyisobutyryl-CoA Ligase (HCL) in~~ the ~~postadenylation state in complex with S3~~-HB-~~AMP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hdy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rohwerder, T]]		[[Category: Rohwerder, T]]
-	[[Category: Zahn, M]]
	[[Category: Strater, N]]		[[Category: Strater, N]]
		+	[[Category: Zahn, M]]
		+	[[Category: 2-hydroxyisobutyrate coa]]
		+	[[Category: Ligase]]

OCA: Protected "6hdy" [edit=sysop:move=sysop]

OCA — Wed, 29 Aug 2018 05:52:31 GMT

Protected "6hdy" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:52, 29 August 2018

OCA: New page: '''Unreleased structure''' The entry 6hdy is ON HOLD Authors: Zahn, M., Rohwerder, T., Strater, N. Description: Crystal structure of 2-Hydroxyisobutyryl-CoA Ligase (HCL) in the postade...

OCA — Wed, 29 Aug 2018 05:52:30 GMT

New page: '''Unreleased structure''' The entry 6hdy is ON HOLD Authors: Zahn, M., Rohwerder, T., Strater, N. Description: Crystal structure of 2-Hydroxyisobutyryl-CoA Ligase (HCL) in the postade...

New page

'''Unreleased structure'''

The entry 6hdy is ON HOLD

Authors: Zahn, M., Rohwerder, T., Strater, N.

Description: Crystal structure of 2-Hydroxyisobutyryl-CoA Ligase (HCL) in the postadenylation state in complex with S3-HB-AMP
[[Category: Unreleased Structures]]
[[Category: Rohwerder, T]]
[[Category: Zahn, M]]
[[Category: Strater, N]]

6hdy - Revision history

OCA at 15:24, 28 August 2019

OCA: Protected "6hdy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6hdy is ON HOLD Authors: Zahn, M., Rohwerder, T., Strater, N. Description: Crystal structure of 2-Hydroxyisobutyryl-CoA Ligase (HCL) in the postade...