6hgr - Revision history

OCA at 06:31, 21 August 2019

OCA — Wed, 21 Aug 2019 06:31:28 GMT

←Older revision		Revision as of 06:31, 21 August 2019
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	<StructureSection load='6hgr' size='340' side='right'caption='[[6hgr]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='6hgr' size='340' side='right'caption='[[6hgr]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hgr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HGR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HGR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hgp\|6hgp]], [[6hgq\|6hgq]], [[6fch\|6fch]], [[6fci\|6fci]], [[6fcl\|6fcl]], [[6fd4\|6fd4]], [[6fd5\|6fd5]], [[6fd6\|6fd6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hgp\|6hgp]], [[6hgq\|6hgq]], [[6fch\|6fch]], [[6fci\|6fci]], [[6fcl\|6fcl]], [[6fd4\|6fd4]], [[6fd5\|6fd5]], [[6fd6\|6fd6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APRT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenine_phosphoribosyltransferase Adenine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.7 2.4.2.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenine_phosphoribosyltransferase Adenine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.7 2.4.2.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hgr OCA], [http://pdbe.org/6hgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hgr RCSB], [http://www.ebi.ac.uk/pdbsum/6hgr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hgr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hgr OCA], [http://pdbe.org/6hgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hgr RCSB], [http://www.ebi.ac.uk/pdbsum/6hgr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hgr ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Adenine phosphoribosyltransferase]]		[[Category: Adenine phosphoribosyltransferase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Huyet, J]]		[[Category: Huyet, J]]

OCA at 06:15, 31 July 2019

OCA — Wed, 31 Jul 2019 06:15:09 GMT

←Older revision		Revision as of 06:15, 31 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hgr is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal Structure of Human APRT wild type in complex with IMP==
		+	<StructureSection load='6hgr' size='340' side='right'caption='[[6hgr]], [[Resolution\|resolution]] 1.52Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hgr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HGR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hgp\|6hgp]], [[6hgq\|6hgq]], [[6fch\|6fch]], [[6fci\|6fci]], [[6fcl\|6fcl]], [[6fd4\|6fd4]], [[6fd5\|6fd5]], [[6fd6\|6fd6]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenine_phosphoribosyltransferase Adenine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.7 2.4.2.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hgr OCA], [http://pdbe.org/6hgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hgr RCSB], [http://www.ebi.ac.uk/pdbsum/6hgr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hgr ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/APT_HUMAN APT_HUMAN]] Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:[http://omim.org/entry/614723 614723]]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.<ref>PMID:1746557</ref> <ref>PMID:7915931</ref> <ref>PMID:3680503</ref> <ref>PMID:3343350</ref> <ref>PMID:1353080</ref> <ref>PMID:11243733</ref> <ref>PMID:15571218</ref> <ref>PMID:21635362</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/APT_HUMAN APT_HUMAN]] Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The reversible adenine phosphoribosyltransferase enzyme (APRT) is essential for purine homeostasis in prokaryotes and eukaryotes. In humans, APRT (hAPRT) is the only enzyme known to produce AMP in cells from dietary adenine. APRT can also process adenine analogs, which are involved in plant development or neuronal homeostasis. However, the molecular mechanism underlying substrate specificity of APRT and catalysis in both directions of the reaction remains poorly understood. Here we present the crystal structures of hAPRT complexed to three cellular nucleotide analogs (Hypoxanthine, IMP and GMP) that we compare to the phosphate-bound enzyme. We established that binding to hAPRT is substrate-shape-specific in the forward reaction, while it is base-specific in the reverse reaction. Furthermore, a quantum mechanics/molecular mechanics (QM/MM) analysis suggests that the forward reaction is mainly a nucleophilic substitution of type 2 (SN2) with a mix of SN1-type molecular mechanism. Based on our structural analysis, a magnesium-assisted SN2-type mechanism would be involved in the reverse reaction. These results provide a framework for understanding the molecular mechanism and substrate discrimination in both directions by APRTs. This knowledge can play an instrumental role in the design of inhibitors, such as antiparasitic agents, or adenine-based substrates.

-	~~Authors: Nioche, P~~., ~~Huyet~~, J., ~~Ozeir~~, M.	+	Structural basis for substrate selectivity and nucleophilic substitution mechanisms in human adenine phosphoribosyltransferase catalyzed reaction.,Ozeir M, Huyet J, Burgevin MC, Pinson B, Chesney F, Remy JM, Siddiqi AR, Lupoli R, Pinon G, Saint-Marc C, Gibert JF, Morales R, Ceballos-Picot I, Barouki R, Daignan-Fornier B, Olivier-Bandini A, Auge F, Nioche P J Biol Chem. 2019 Jun 3. pii: RA119.009087. doi: 10.1074/jbc.RA119.009087. PMID:31160323<ref>PMID:31160323</ref>

-	~~Description: Crystal Structure~~ of ~~Human APRT wild type in complex with IMP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Ozeir, M~~]]	+	<div class="pdbe-citations 6hgr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adenine phosphoribosyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Huyet, J]]		[[Category: Huyet, J]]
	[[Category: Nioche, P]]		[[Category: Nioche, P]]
		+	[[Category: Ozeir, M]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Transferase]]

OCA at 04:10, 24 July 2019

OCA — Wed, 24 Jul 2019 04:10:22 GMT

←Older revision		Revision as of 04:10, 24 July 2019
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	Authors: Nioche, P., Huyet, J., Ozeir, M.		Authors: Nioche, P., Huyet, J., Ozeir, M.

-	Description: Crystal Structure of ~~ligand4 complexed to a PRT Family Member~~	+	Description: Crystal Structure of Human APRT wild type in complex with IMP
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ozeir, M]]		[[Category: Ozeir, M]]
	[[Category: Huyet, J]]		[[Category: Huyet, J]]
	[[Category: Nioche, P]]		[[Category: Nioche, P]]

OCA: Protected "6hgr" [edit=sysop:move=sysop]

OCA — Wed, 29 Aug 2018 06:02:06 GMT

Protected "6hgr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 29 August 2018

OCA: New page: '''Unreleased structure''' The entry 6hgr is ON HOLD until Paper Publication Authors: Nioche, P., Huyet, J., Ozeir, M. Description: Crystal Structure of ligand4 complexed to a PRT Fami...

OCA — Wed, 29 Aug 2018 06:02:05 GMT

New page: '''Unreleased structure''' The entry 6hgr is ON HOLD until Paper Publication Authors: Nioche, P., Huyet, J., Ozeir, M. Description: Crystal Structure of ligand4 complexed to a PRT Fami...

New page

'''Unreleased structure'''

The entry 6hgr is ON HOLD until Paper Publication

Authors: Nioche, P., Huyet, J., Ozeir, M.

Description: Crystal Structure of ligand4 complexed to a PRT Family Member
[[Category: Unreleased Structures]]
[[Category: Ozeir, M]]
[[Category: Huyet, J]]
[[Category: Nioche, P]]