6hh9 - Revision history

OCA at 07:14, 8 April 2020

OCA — Wed, 08 Apr 2020 07:14:16 GMT

←Older revision		Revision as of 07:14, 8 April 2020
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	<StructureSection load='6hh9' size='340' side='right'caption='[[6hh9]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6hh9' size='340' side='right'caption='[[6hh9]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hh9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Roseburia_intestinalis_l1-82 Roseburia intestinalis l1-82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HH9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HH9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hh9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Roseburia_intestinalis_l1-82 Roseburia intestinalis l1-82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HH9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HH9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hfz\|6hfz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hfz\|6hfz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ROSINTL182_05471 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536231 Roseburia intestinalis L1-82])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ROSINTL182_05471 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536231 Roseburia intestinalis L1-82])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hh9 OCA], [http://pdbe.org/6hh9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hh9 RCSB], [http://www.ebi.ac.uk/pdbsum/6hh9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hh9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hh9 OCA], [http://pdbe.org/6hh9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hh9 RCSB], [http://www.ebi.ac.uk/pdbsum/6hh9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hh9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:30, 27 March 2020

OCA — Fri, 27 Mar 2020 10:30:04 GMT

←Older revision		Revision as of 10:30, 27 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6hh9 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a two-domain esterase (CEX) active on acetylated mannans co-crystallized with mannopentaose==
		+	<StructureSection load='6hh9' size='340' side='right'caption='[[6hh9]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hh9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Roseburia_intestinalis_l1-82 Roseburia intestinalis l1-82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HH9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HH9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hfz\|6hfz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ROSINTL182_05471 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536231 Roseburia intestinalis L1-82])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hh9 OCA], [http://pdbe.org/6hh9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hh9 RCSB], [http://www.ebi.ac.uk/pdbsum/6hh9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hh9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-mannans and xylans are important components of the plant cell wall and they are acetylated to be protected from degradation by glycoside hydrolases. beta-mannans are widely present in human and animal diets as fiber from leguminous plants and as thickeners and stabilizers in processed foods. There are many fully characterized acetylxylan esterases (AcXEs); however, the enzymes deacetylating mannans are less understood. Here we present two carbohydrate esterases, RiCE2 and RiCE17, from the Firmicute Roseburia intestinalis, which together deacetylate complex galactoglucomannan (GGM). The three-dimensional (3D) structure of RiCE17 with a mannopentaose in the active site shows that the CBM35 domain of RiCE17 forms a confined complex, where the axially oriented C2-hydroxyl of a mannose residue points toward the Ser41 of the catalytic triad. Cavities on the RiCE17 surface may accept galactosylations at the C6 positions of mannose adjacent to the mannose residue being deacetylated (subsite -1 and +1). In-depth characterization of the two enzymes using time-resolved NMR, high-performance liquid chromatography (HPLC), and mass spectrometry demonstrates that they work in a complementary manner. RiCE17 exclusively removes the axially oriented 2-O-acetylations on any mannose residue in an oligosaccharide, including double acetylated mannoses, while the RiCE2 is active on 3-O-, 4-O-, and 6-O-acetylations. Activity of RiCE2 is dependent on RiCE17 removing 2-O-acetylations from double acetylated mannose. Furthermore, transacetylation of oligosaccharides with the 2-O-specific RiCE17 provided insight into how temperature and pH affects acetyl migration on manno-oligosaccharides.

-	~~Authors: Michalak~~, L., La Rosa, S~~.L.~~, Rohr, A.K., ~~Aachmann, F~~.L.~~, Westereng, B~~.	+	A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex beta-mannans.,Michalak L, La Rosa SL, Leivers S, Lindstad LJ, Rohr AK, Lillelund Aachmann F, Westereng B Proc Natl Acad Sci U S A. 2020 Mar 13. pii: 1915376117. doi:, 10.1073/pnas.1915376117. PMID:32170022<ref>PMID:32170022</ref>

-	~~Description: Crystal structure of~~ a ~~two~~-~~domain esterase (CEX) active on acetylated mannans co~~-~~crystallized with mannopentaose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hh9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Roseburia intestinalis l1-82]]
		+	[[Category: Aachmann, F L]]
	[[Category: Michalak, L]]		[[Category: Michalak, L]]
-	[[Category: ~~Aachmann~~, F.L]]	+	[[Category: Rohr, A K]]
		+	[[Category: Rosa, S L.La]]
	[[Category: Westereng, B]]		[[Category: Westereng, B]]
-	[[Category: ~~Rohr, A.K~~]]	+	[[Category: Biomass]]
-	[[Category: ~~La Rosa, S.L~~]]	+	[[Category: Carbohydrate]]
		+	[[Category: Deacetylation]]
		+	[[Category: Esterase]]
		+	[[Category: Galactoglucomannan]]
		+	[[Category: Gut flora]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "6hh9" [edit=sysop:move=sysop]

OCA — Wed, 05 Sep 2018 08:44:59 GMT

Protected "6hh9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:44, 5 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hh9 is ON HOLD until Paper Publication Authors: Michalak, L., La Rosa, S.L., Rohr, A.K., Aachmann, F.L., Westereng, B. Description: Crystal struct...

OCA — Wed, 05 Sep 2018 08:44:57 GMT

New page: '''Unreleased structure''' The entry 6hh9 is ON HOLD until Paper Publication Authors: Michalak, L., La Rosa, S.L., Rohr, A.K., Aachmann, F.L., Westereng, B. Description: Crystal struct...

New page

'''Unreleased structure'''

The entry 6hh9 is ON HOLD until Paper Publication

Authors: Michalak, L., La Rosa, S.L., Rohr, A.K., Aachmann, F.L., Westereng, B.

Description: Crystal structure of a two-domain esterase (CEX) active on acetylated mannans co-crystallized with mannopentaose
[[Category: Unreleased Structures]]
[[Category: Michalak, L]]
[[Category: Aachmann, F.L]]
[[Category: Westereng, B]]
[[Category: Rohr, A.K]]
[[Category: La Rosa, S.L]]