6his - Revision history

OCA at 07:00, 14 September 2022

OCA — Wed, 14 Sep 2022 07:00:17 GMT

←Older revision		Revision as of 07:00, 14 September 2022
Line 3:		Line 3:
	<SX load='6his' size='340' side='right' viewer='molstar' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>		<SX load='6his' size='340' side='right' viewer='molstar' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6HIS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HIS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Htr3a, 5ht3, Htr3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [https://pdbe.org/6his PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [https://www.ebi.ac.uk/pdbsum/6his PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [~~http~~://pdbe.org/6his PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6his PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/5HT3A_MOUSE 5HT3A_MOUSE]] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.	+	[[https://www.uniprot.org/uniprot/5HT3A_MOUSE 5HT3A_MOUSE]] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Neumann, E]]	+	[[Category: Neumann E]]
-	[[Category: Nury, H]]	+	[[Category: Nury H]]
-	[[Category: Polovinkin, L]]	+	[[Category: Polovinkin L]]
-	[[Category: Schoehn, G]]	+	[[Category: Schoehn G]]
-	~~[[Category: Ion channel]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pentameric ligand-gated channel]]~~	+
-	~~[[Category: Serotonin receptor~~]]	+

OCA at 01:23, 11 April 2020

OCA — Sat, 11 Apr 2020 01:23:52 GMT

←Older revision		Revision as of 01:23, 11 April 2020
Line 3:		Line 3:
	<SX load='6his' size='340' side='right' viewer='molstar' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>		<SX load='6his' size='340' side='right' viewer='molstar' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HIS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HIS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Htr3a, 5ht3, Htr3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Htr3a, 5ht3, Htr3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [http://pdbe.org/6his PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [http://www.ebi.ac.uk/pdbsum/6his PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [http://pdbe.org/6his PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [http://www.ebi.ac.uk/pdbsum/6his PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:42, 6 March 2020

OCA — Fri, 06 Mar 2020 21:42:40 GMT

←Older revision		Revision as of 21:42, 6 March 2020
Line 1:		Line 1:

	==Mouse serotonin 5-HT3 receptor, tropisetron-bound, T conformation==		==Mouse serotonin 5-HT3 receptor, tropisetron-bound, T conformation==
-	<~~StructureSection~~ load='6his' size='340' side='right' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>	+	<SX load='6his' size='340' side='right' viewer='molstar' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HIS FirstGlance]. <br>		<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HIS FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6his" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6his" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[5-hydroxytryptamine receptor 3D structures\|5-hydroxytryptamine receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Neumann, E]]		[[Category: Neumann, E]]

OCA at 21:05, 2 December 2018

OCA — Sun, 02 Dec 2018 21:05:11 GMT

←Older revision		Revision as of 21:05, 2 December 2018
Line 3:		Line 3:
	<StructureSection load='6his' size='340' side='right' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>		<StructureSection load='6his' size='340' side='right' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HIS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HIS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Htr3a, 5ht3, Htr3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [http://pdbe.org/6his PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [http://www.ebi.ac.uk/pdbsum/6his PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [http://pdbe.org/6his PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [http://www.ebi.ac.uk/pdbsum/6his PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/5HT3A_MOUSE 5HT3A_MOUSE]] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.		[[http://www.uniprot.org/uniprot/5HT3A_MOUSE 5HT3A_MOUSE]] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The serotonin 5-HT3 receptor is a pentameric ligand-gated ion channel (pLGIC). It belongs to a large family of receptors that function as allosteric signal transducers across the plasma membrane(1,2); upon binding of neurotransmitter molecules to extracellular sites, the receptors undergo complex conformational transitions that result in transient opening of a pore permeable to ions. 5-HT3 receptors are therapeutic targets for emesis and nausea, irritable bowel syndrome and depression(3). In spite of several reported pLGIC structures(4-8), no clear unifying view has emerged on the conformational transitions involved in channel gating. Here we report four cryo-electron microscopy structures of the full-length mouse 5-HT3 receptor in complex with the anti-emetic drug tropisetron, with serotonin, and with serotonin and a positive allosteric modulator, at resolutions ranging from 3.2 A to 4.5 A. The tropisetron-bound structure resembles those obtained with an inhibitory nanobody(5) or without ligand(9). The other structures include an 'open' state and two ligand-bound states. We present computational insights into the dynamics of the structures, their pore hydration and free-energy profiles, and characterize movements at the gate level and cation accessibility in the pore. Together, these data deepen our understanding of the gating mechanism of pLGICs and capture ligand binding in unprecedented detail.
		+
		+	Conformational transitions of the serotonin 5-HT3 receptor.,Polovinkin L, Hassaine G, Perot J, Neumann E, Jensen AA, Lefebvre SN, Corringer PJ, Neyton J, Chipot C, Dehez F, Schoehn G, Nury H Nature. 2018 Nov;563(7730):275-279. doi: 10.1038/s41586-018-0672-3. Epub 2018 Oct, 31. PMID:30401839<ref>PMID:30401839</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6his" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Neumann, E]]		[[Category: Neumann, E]]
	[[Category: Nury, H]]		[[Category: Nury, H]]

OCA at 12:22, 7 November 2018

OCA — Wed, 07 Nov 2018 12:22:16 GMT

←Older revision		Revision as of 12:22, 7 November 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6his is ~~ON HOLD~~	+	==Mouse serotonin 5-HT3 receptor, tropisetron-bound, T conformation==
-		+	<StructureSection load='6his' size='340' side='right' caption='[[6his]], [[Resolution\|resolution]] 4.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6his]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HIS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TKT:(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+1H-INDOLE-3-CARBOXYLATE'>TKT</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6his FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6his OCA], [http://pdbe.org/6his PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6his RCSB], [http://www.ebi.ac.uk/pdbsum/6his PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6his ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/5HT3A_MOUSE 5HT3A_MOUSE]] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Neumann, E]]
		+	[[Category: Nury, H]]
		+	[[Category: Polovinkin, L]]
		+	[[Category: Schoehn, G]]
		+	[[Category: Ion channel]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pentameric ligand-gated channel]]
		+	[[Category: Serotonin receptor]]

OCA: Protected "6his" [edit=sysop:move=sysop]

OCA — Wed, 05 Sep 2018 08:49:28 GMT

Protected "6his" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:49, 5 September 2018

OCA: New page: '''Unreleased structure''' The entry 6his is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Sep 2018 08:49:27 GMT

New page: '''Unreleased structure''' The entry 6his is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6his is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]