6hjc - Revision history

OCA at 07:04, 24 October 2018

OCA — Wed, 24 Oct 2018 07:04:56 GMT

←Older revision		Revision as of 07:04, 24 October 2018
Line 3:		Line 3:
	<StructureSection load='6hjc' size='340' side='right' caption='[[6hjc]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='6hjc' size='340' side='right' caption='[[6hjc]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hjc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loei_river_mammarenavirus Loei river mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1437126 Loei River mammarenavirus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjc OCA], [http://pdbe.org/6hjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hjc RCSB], [http://www.ebi.ac.uk/pdbsum/6hjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjc OCA], [http://pdbe.org/6hjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hjc RCSB], [http://www.ebi.ac.uk/pdbsum/6hjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/A0A023J4Z7_9VIRU A0A023J4Z7_9VIRU]] Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084]		[[http://www.uniprot.org/uniprot/A0A023J4Z7_9VIRU A0A023J4Z7_9VIRU]] Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The emergence of Old and New World arenaviruses from rodent reservoirs persistently threatens human health. The GP1 subunit of the envelope-displayed arenaviral glycoprotein spike complex, GPC, mediates host-cell recognition and is an important determinant of cross-species transmission. Previous structural analyses of Old World arenaviral GP1 glycoproteins, alone and in complex with a cognate GP2 subunit, have revealed that GP1 adopts two distinct conformational states, distinguished by differences in orientation of helical regions of the molecule. Here, through comparative study of the GP1 glycoprotein architectures of Old World Loei River virus and New World Whitewater Arroyo virus, we show that these rearrangements are restricted to Old World arenaviruses and are not solely induced by the pH change that is associated with virus endosomal trafficking. Our structure-based phylogenetic analysis of arenaviral GP1s provides a blueprint for understanding the discrete structural classes adopted by these therapeutically important targets.IMPORTANCE The genetically and geographically diverse group of viruses within the Arenaviridae family includes a number of zoonotic pathogens capable of causing fatal hemorrhagic fever. The multi-subunit GPC glycoprotein spike complex displayed on the arenavirus envelope is a key determinant of species tropism and the primary target of the host humoral immune response. Here, we show that the receptor-binding GP1 sub-component of the GPC spike from Old World but not New World arenaviruses adopts a distinct, pH-independent conformation in the absence of the cognate GP2. Our analysis provides a structure-based approach for understanding the discrete conformational classes sampled by these therapeutically important targets, informing strategies to develop arenaviral glycoprotein immunogens that resemble GPC, as presented on the mature virion surface.
		+
		+	Structure-based classification defines the discrete conformational classes adopted by the arenaviral GP1.,Pryce R, Ng WM, Zeltina A, Watanabe Y, El Omari K, Wagner A, Bowden TA J Virol. 2018 Oct 10. pii: JVI.01048-18. doi: 10.1128/JVI.01048-18. PMID:30305351<ref>PMID:30305351</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hjc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Loei river mammarenavirus]]
	[[Category: Bowden, T A]]		[[Category: Bowden, T A]]
	[[Category: Ng, W M]]		[[Category: Ng, W M]]

OCA at 08:16, 10 October 2018

OCA — Wed, 10 Oct 2018 08:16:21 GMT

←Older revision		Revision as of 08:16, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hjc ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Loei River virus GP1 glycoprotein at pH 8.0==
-		+	<StructureSection load='6hjc' size='340' side='right' caption='[[6hjc]], [[Resolution\|resolution]] 2.51Å' scene=''>
-	~~Authors~~: ~~Pryce, R~~., ~~Ng, W~~.M., ~~Zeltina, A~~., ~~Watanabe, Y~~., ~~El Omari, K~~., ~~Wagner, A~~., ~~Bowden, T~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hjc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJC FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~Loei River virus~~ GP1 glycoprotein at pH 8.0	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hjc OCA], [http://pdbe.org/6hjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hjc RCSB], [http://www.ebi.ac.uk/pdbsum/6hjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hjc ProSAT]</span></td></tr>
-	[[Category: ~~El Omari~~, K]]	+	</table>
-	[[Category: ~~Zeltina~~, A]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A023J4Z7_9VIRU A0A023J4Z7_9VIRU]] Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bowden, T A]]
		+	[[Category: Ng, W M]]
		+	[[Category: Omari, K El]]
	[[Category: Pryce, R]]		[[Category: Pryce, R]]
	[[Category: Wagner, A]]		[[Category: Wagner, A]]
-	[[Category: Bowden, T.A]]
-	[[Category: Ng, W.M]]
	[[Category: Watanabe, Y]]		[[Category: Watanabe, Y]]
		+	[[Category: Zeltina, A]]
		+	[[Category: Attachment]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Viral protein]]

OCA at 06:09, 26 September 2018

OCA — Wed, 26 Sep 2018 06:09:03 GMT

←Older revision		Revision as of 06:09, 26 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hjc is ON HOLD	+	The entry 6hjc is ON HOLD until Paper Publication

	Authors: Pryce, R., Ng, W.M., Zeltina, A., Watanabe, Y., El Omari, K., Wagner, A., Bowden, T.A.		Authors: Pryce, R., Ng, W.M., Zeltina, A., Watanabe, Y., El Omari, K., Wagner, A., Bowden, T.A.
Line 12:		Line 12:
	[[Category: Wagner, A]]		[[Category: Wagner, A]]
	[[Category: Bowden, T.A]]		[[Category: Bowden, T.A]]
-	[[Category: Watanabe, Y]]
	[[Category: Ng, W.M]]		[[Category: Ng, W.M]]
		+	[[Category: Watanabe, Y]]

OCA: Protected "6hjc" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2018 06:03:38 GMT

Protected "6hjc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:03, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hjc is ON HOLD Authors: Pryce, R., Ng, W.M., Zeltina, A., Watanabe, Y., El Omari, K., Wagner, A., Bowden, T.A. Description: Crystal structure of L...

OCA — Wed, 12 Sep 2018 06:03:36 GMT

New page: '''Unreleased structure''' The entry 6hjc is ON HOLD Authors: Pryce, R., Ng, W.M., Zeltina, A., Watanabe, Y., El Omari, K., Wagner, A., Bowden, T.A. Description: Crystal structure of L...

New page

'''Unreleased structure'''

The entry 6hjc is ON HOLD

Authors: Pryce, R., Ng, W.M., Zeltina, A., Watanabe, Y., El Omari, K., Wagner, A., Bowden, T.A.

Description: Crystal structure of Loei River virus GP1 glycoprotein at pH 8.0
[[Category: Unreleased Structures]]
[[Category: El Omari, K]]
[[Category: Zeltina, A]]
[[Category: Pryce, R]]
[[Category: Wagner, A]]
[[Category: Bowden, T.A]]
[[Category: Watanabe, Y]]
[[Category: Ng, W.M]]