6hk6 - Revision history

OCA at 07:57, 24 April 2019

OCA — Wed, 24 Apr 2019 07:57:33 GMT

←Older revision		Revision as of 07:57, 24 April 2019
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RIOK2_HUMAN RIOK2_HUMAN]] Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).<ref>PMID:16037817</ref> <ref>PMID:19564402</ref> <ref>PMID:21880710</ref>		[[http://www.uniprot.org/uniprot/RIOK2_HUMAN RIOK2_HUMAN]] Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).<ref>PMID:16037817</ref> <ref>PMID:19564402</ref> <ref>PMID:21880710</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RIO kinases (RIOKs) are a universal family of atypical kinases that are essential for assembly of the pre-40S ribosome complex. Here, we present the crystal structure of human RIO kinase 2 (RIOK2) bound to a specific inhibitor. This first crystal structure of an inhibitor-bound RIO kinase reveals the binding mode of the inhibitor and explains the structure-activity relationship of the inhibitor series. The inhibitor binds in the ATP-binding site and forms extensive hydrophobic interactions with residues at the entrance to the ATP-binding site. Analysis of the conservation of active site residues reveals the reasons for the specificity of the inhibitor for RIOK2 over RIOK1 and RIOK3, and it provides a template for inhibitor design against the human RIOK family.
		+
		+	Crystal structure of human RIOK2 bound to a specific inhibitor.,Wang J, Varin T, Vieth M, Elkins JM Open Biol. 2019 Apr 26;9(4):190037. doi: 10.1098/rsob.190037. PMID:30991936<ref>PMID:30991936</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hk6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:16, 3 April 2019

OCA — Wed, 03 Apr 2019 08:16:23 GMT

←Older revision		Revision as of 08:16, 3 April 2019
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	==Human RIOK2 bound to inhibitor==		==Human RIOK2 bound to inhibitor==
-	<StructureSection load='6hk6' size='340' side='right' caption='[[6hk6]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='6hk6' size='340' side='right'caption='[[6hk6]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hk6]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HK6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hk6]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HK6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=G8H:2-naphthalen-2-yl-~{N}-pyridin-2-yl-ethanamide'>G8H</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=G8H:2-naphthalen-2-yl-~{N}-pyridin-2-yl-ethanamide'>G8H</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RIOK2, RIO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hk6 OCA], [http://pdbe.org/6hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/6hk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hk6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hk6 OCA], [http://pdbe.org/6hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/6hk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hk6 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 06:04, 24 October 2018

OCA — Wed, 24 Oct 2018 06:04:31 GMT

←Older revision		Revision as of 06:04, 24 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hk6 ~~is ON HOLD~~	+	==Human RIOK2 bound to inhibitor==
-		+	<StructureSection load='6hk6' size='340' side='right' caption='[[6hk6]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~: ~~Wang, J~~., ~~Krojer~~, T., ~~Bountra~~, C., ~~Edwards~~, A.M., ~~Arrowsmith~~, C.~~, Knapp, S~~., ~~Elkins~~, J.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hk6]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HK6 FirstGlance]. <br>
-	~~Description: Human RIOK2 bound to inhibitor~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=G8H:2-naphthalen-2-yl-~{N}-pyridin-2-yl-ethanamide'>G8H</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	[[Category: ~~Elkins, J.M~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hk6 OCA], [http://pdbe.org/6hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/6hk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hk6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RIOK2_HUMAN RIOK2_HUMAN]] Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).<ref>PMID:16037817</ref> <ref>PMID:19564402</ref> <ref>PMID:21880710</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]
		+	[[Category: Bountra, C]]
		+	[[Category: Edwards, A M]]
		+	[[Category: Elkins, J M]]
	[[Category: Knapp, S]]		[[Category: Knapp, S]]
-	[[Category: Edwards, A.M]]
	[[Category: Krojer, T]]		[[Category: Krojer, T]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
-	[[Category: ~~Bountra, C~~]]	+	[[Category: Kinase]]
		+	[[Category: Sgc]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]

OCA: Protected "6hk6" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2018 06:06:15 GMT

Protected "6hk6" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hk6 is ON HOLD Authors: Wang, J., Krojer, T., Bountra, C., Edwards, A.M., Arrowsmith, C., Knapp, S., Elkins, J.M. Description: Human RIOK2 bound t...

OCA — Wed, 12 Sep 2018 06:06:14 GMT

New page: '''Unreleased structure''' The entry 6hk6 is ON HOLD Authors: Wang, J., Krojer, T., Bountra, C., Edwards, A.M., Arrowsmith, C., Knapp, S., Elkins, J.M. Description: Human RIOK2 bound t...

New page

'''Unreleased structure'''

The entry 6hk6 is ON HOLD

Authors: Wang, J., Krojer, T., Bountra, C., Edwards, A.M., Arrowsmith, C., Knapp, S., Elkins, J.M.

Description: Human RIOK2 bound to inhibitor
[[Category: Unreleased Structures]]
[[Category: Elkins, J.M]]
[[Category: Arrowsmith, C]]
[[Category: Knapp, S]]
[[Category: Edwards, A.M]]
[[Category: Krojer, T]]
[[Category: Wang, J]]
[[Category: Bountra, C]]