6hke - Revision history

OCA at 06:23, 2 January 2019

OCA — Wed, 02 Jan 2019 06:23:11 GMT

←Older revision		Revision as of 06:23, 2 January 2019
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	<StructureSection load='6hke' size='340' side='right' caption='[[6hke]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='6hke' size='340' side='right' caption='[[6hke]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hke]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HKE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hke]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopa Rhopa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HKE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RPA3494 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=258594 RHOPA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hke OCA], [http://pdbe.org/6hke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hke RCSB], [http://www.ebi.ac.uk/pdbsum/6hke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hke ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hke OCA], [http://pdbe.org/6hke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hke RCSB], [http://www.ebi.ac.uk/pdbsum/6hke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hke ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Rhopa]]
	[[Category: Dix, S]]		[[Category: Dix, S]]
	[[Category: Kelly, D J]]		[[Category: Kelly, D J]]

OCA at 06:46, 5 December 2018

OCA — Wed, 05 Dec 2018 06:46:25 GMT

←Older revision		Revision as of 06:46, 5 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6hke is ~~ON HOLD~~	+	==MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate==
		+	<StructureSection load='6hke' size='340' side='right' caption='[[6hke]], [[Resolution\|resolution]] 2.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hke]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HKE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hke OCA], [http://pdbe.org/6hke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hke RCSB], [http://www.ebi.ac.uk/pdbsum/6hke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hke ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	C4-dicarboxylates play a central role in cellular physiology as key metabolic intermediates. Under aerobic conditions, they participate in the citric-acid cycle, while in anaerobic bacteria, they are important in energy-conserving fermentation and respiration processes. Ten different families of secondary transporters have been described to participate in C4-dicarboxylate movement across biological membranes but only one of these utilizes an extracytoplasmic solute binding protein to achieve high-affinity uptake. Here, we identify the MatBAC system from the photosynthetic bacterium Rhodopseudomonas palustris as the first member of the Tripartite Tricarboxylate Transport (TTT) family to be involved in C4-dicarboxylate transport. Tryptophan fluorescence spectroscopy showed that MatC, the periplasmic binding protein from this system, binds to L- and D-malate with Kd values of 27 and 21nM respectively, the highest reported affinity to date for these C4-dicarboxylates, and to succinate (Kd 110nM) and fumarate (Kd 400nM). The 2.1A crystal structure of MatC with bound malate shows a high level of substrate coordination, with participation of two water molecules that bridge hydrogen bonds between the ligand proximal carboxylic group and the main chain of two conserved loops in the protein structure. The substrate coordination in MatC correlates with the binding data, and explains the protein's selectivity for different substrates and respective binding affinities. Our results reveal a new function in C4-dicarboxylate transport by members of the poorly characterized TTT family, which are widely distributed in bacterial genomes but for which details of structure-function relationships and transport mechanisms have been lacking.

-	~~Authors: Rosa~~, ~~L.T~~., Dix~~, S.~~, Rafferty, J.B., ~~Kelly, D~~.J.	+	A New Mechanism for High-Affinity Uptake of C4-Dicarboxylates in Bacteria Revealed by the Structure of Rhodopseudomonas palustris MatC (RPA3494), a Periplasmic Binding-Protein of the Tripartite Tricarboxylate Transporter (TTT) Family.,Rosa LT, Dix SR, Rafferty JB, Kelly DJ J Mol Biol. 2018 Nov 21. pii: S0022-2836(18)30977-X. doi:, 10.1016/j.jmb.2018.11.016. PMID:30471256<ref>PMID:30471256</ref>

-	~~Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6hke" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dix, S]]		[[Category: Dix, S]]
-	[[Category: Kelly, D.J]]	+	[[Category: Kelly, D J]]
-	[[Category: Rafferty, J.B]]	+	[[Category: Rafferty, J B]]
-	[[Category: Rosa, L.T]]	+	[[Category: Rosa, L T]]
		+	[[Category: Malate uptake]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Transport protein]]
		+	[[Category: Ttt family]]
		+	[[Category: Type ii class]]

OCA at 18:28, 2 December 2018

OCA — Sun, 02 Dec 2018 18:28:01 GMT

←Older revision		Revision as of 18:28, 2 December 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hke is ON HOLD ~~until Paper Publication~~	+	The entry 6hke is ON HOLD

	Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J.		Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J.
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	Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate		Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Rafferty, J.B]]
-	[[Category: Rosa, L.T]]
	[[Category: Dix, S]]		[[Category: Dix, S]]
	[[Category: Kelly, D.J]]		[[Category: Kelly, D.J]]
		+	[[Category: Rafferty, J.B]]
		+	[[Category: Rosa, L.T]]

OCA at 18:09, 19 September 2018

OCA — Wed, 19 Sep 2018 18:09:00 GMT

←Older revision		Revision as of 18:09, 19 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hke is ON HOLD	+	The entry 6hke is ON HOLD until Paper Publication

	Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J.		Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rafferty, J.B]]		[[Category: Rafferty, J.B]]
-	[[Category: Kelly, D.J]]
	[[Category: Rosa, L.T]]		[[Category: Rosa, L.T]]
	[[Category: Dix, S]]		[[Category: Dix, S]]
		+	[[Category: Kelly, D.J]]

OCA: Protected "6hke" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2018 06:06:48 GMT

Protected "6hke" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hke is ON HOLD Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J. Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound ma...

OCA — Wed, 12 Sep 2018 06:06:47 GMT

New page: '''Unreleased structure''' The entry 6hke is ON HOLD Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J. Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound ma...

New page

'''Unreleased structure'''

The entry 6hke is ON HOLD

Authors: Rosa, L.T., Dix, S., Rafferty, J.B., Kelly, D.J.

Description: MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate
[[Category: Unreleased Structures]]
[[Category: Rafferty, J.B]]
[[Category: Kelly, D.J]]
[[Category: Rosa, L.T]]
[[Category: Dix, S]]