6hkq - Revision history

OCA at 08:25, 7 December 2022

OCA — Wed, 07 Dec 2022 08:25:55 GMT

←Older revision		Revision as of 08:25, 7 December 2022
Line 3:		Line 3:
	<StructureSection load='6hkq' size='340' side='right'caption='[[6hkq]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='6hkq' size='340' side='right'caption='[[6hkq]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G9N:(2~{S})-2-[2-chloranylethanoyl-(3-chloranyl-4-methoxy-phenyl)amino]-~{N}-(2-phenylethyl)-2-thiophen-2-yl-ethanamide'>G9N</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G9N:(2~{S})-2-[2-chloranylethanoyl-(3-chloranyl-4-methoxy-phenyl)amino]-~{N}-(2-phenylethyl)-2-thiophen-2-yl-ethanamide'>G9N</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6hn3\|6hn3]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPX4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipid-hydroperoxide_glutathione_peroxidase Phospholipid-hydroperoxide glutathione peroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.12 1.11.1.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hkq OCA], [https://pdbe.org/6hkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hkq RCSB], [https://www.ebi.ac.uk/pdbsum/6hkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hkq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hkq OCA], [https://pdbe.org/6hkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hkq RCSB], [https://www.ebi.ac.uk/pdbsum/6hkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GPX4_HUMAN GPX4_HUMAN]] Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).	+	[https://www.uniprot.org/uniprot/GPX4_HUMAN GPX4_HUMAN] Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phospholipid-hydroperoxide glutathione peroxidase]]~~	+	[[Category: Badock V]]
-	[[Category: Badock, V]]	+	[[Category: Eaton JK]]
-	[[Category: Eaton~~, J K~~]]	+	[[Category: Gradl S]]
-	[[Category: Gradl, S]]	+	[[Category: Hillig RC]]
-	[[Category: Hillig~~, R C~~]]	+	[[Category: Hilpmann A]]
-	[[Category: Hilpmann, A]]	+	[[Category: Hoffmann L]]
-	[[Category: Hoffmann, L]]	+	[[Category: Moosmayer D]]
-	[[Category: Moosmayer, D]]	+	[[Category: Schnirch L]]
-	[[Category: Schnirch, L]]	+
-	~~[[Category: Anti-oxidatve defense system]]~~	+
-	~~[[Category: Covalent inhibitor]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 14:49, 17 June 2021

OCA — Thu, 17 Jun 2021 14:49:54 GMT

←Older revision		Revision as of 14:49, 17 June 2021
Line 3:		Line 3:
	<StructureSection load='6hkq' size='340' side='right'caption='[[6hkq]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='6hkq' size='340' side='right'caption='[[6hkq]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hkq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G9N:(2~{S})-2-[2-chloranylethanoyl-(3-chloranyl-4-methoxy-phenyl)amino]-~{N}-(2-phenylethyl)-2-thiophen-2-yl-ethanamide'>G9N</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G9N:(2~{S})-2-[2-chloranylethanoyl-(3-chloranyl-4-methoxy-phenyl)amino]-~{N}-(2-phenylethyl)-2-thiophen-2-yl-ethanamide'>G9N</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hn3\|6hn3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6hn3\|6hn3]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phospholipid-hydroperoxide_glutathione_peroxidase Phospholipid-hydroperoxide glutathione peroxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.12 1.11.1.12] </span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPX4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hkq OCA], [~~http~~://pdbe.org/6hkq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hkq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hkq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hkq ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipid-hydroperoxide_glutathione_peroxidase Phospholipid-hydroperoxide glutathione peroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.12 1.11.1.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hkq OCA], [https://pdbe.org/6hkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hkq RCSB], [https://www.ebi.ac.uk/pdbsum/6hkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GPX4_HUMAN GPX4_HUMAN]] Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).	+	[[https://www.uniprot.org/uniprot/GPX4_HUMAN GPX4_HUMAN]] Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Wild-type human glutathione peroxidase 4 (GPX4) was co-expressed with SBP2 (selenocysteine insertion sequence-binding protein 2) in human HEK cells to achieve efficient production of this selenocysteine-containing enzyme on a preparative scale for structural biology. The protein was purified and crystallized, and the crystal structure of the wild-type form of GPX4 was determined at 1.0 A resolution. The overall fold and the active site are conserved compared with previously determined crystal structures of mutated forms of GPX4. A mass-spectrometry-based approach was developed to monitor the reaction of the active-site selenocysteine Sec46 with covalent inhibitors. This, together with the introduction of a surface mutant (Cys66Ser), enabled the crystal structure determination of GPX4 in complex with the covalent inhibitor ML162 [(S)-enantiomer]. The mass-spectrometry-based approach described here opens the path to further co-complex crystal structures of this potential cancer drug target in complex with covalent inhibitors.
		+
		+	Crystal structures of the selenoprotein glutathione peroxidase 4 in its apo form and in complex with the covalently bound inhibitor ML162.,Moosmayer D, Hilpmann A, Hoffmann J, Schnirch L, Zimmermann K, Badock V, Furst L, Eaton JK, Viswanathan VS, Schreiber SL, Gradl S, Hillig RC Acta Crystallogr D Struct Biol. 2021 Feb 1;77(Pt 2):237-248. doi:, 10.1107/S2059798320016125. Epub 2021 Jan 26. PMID:33559612<ref>PMID:33559612</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hkq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione peroxidase\|Glutathione peroxidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Phospholipid-hydroperoxide glutathione peroxidase]]		[[Category: Phospholipid-hydroperoxide glutathione peroxidase]]

OCA at 09:04, 1 April 2020

OCA — Wed, 01 Apr 2020 09:04:32 GMT

←Older revision		Revision as of 09:04, 1 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hkq is ~~ON HOLD until Paper Publication~~	+	==Human GPX4 in complex with covalent Inhibitor ML162 (S enantiomer)==
-		+	<StructureSection load='6hkq' size='340' side='right'caption='[[6hkq]], [[Resolution\|resolution]] 1.54Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hkq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HKQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G9N:(2~{S})-2-[2-chloranylethanoyl-(3-chloranyl-4-methoxy-phenyl)amino]-~{N}-(2-phenylethyl)-2-thiophen-2-yl-ethanamide'>G9N</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hn3\|6hn3]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipid-hydroperoxide_glutathione_peroxidase Phospholipid-hydroperoxide glutathione peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.12 1.11.1.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hkq OCA], [http://pdbe.org/6hkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hkq RCSB], [http://www.ebi.ac.uk/pdbsum/6hkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hkq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GPX4_HUMAN GPX4_HUMAN]] Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Phospholipid-hydroperoxide glutathione peroxidase]]
		+	[[Category: Badock, V]]
		+	[[Category: Eaton, J K]]
		+	[[Category: Gradl, S]]
		+	[[Category: Hillig, R C]]
		+	[[Category: Hilpmann, A]]
		+	[[Category: Hoffmann, L]]
		+	[[Category: Moosmayer, D]]
		+	[[Category: Schnirch, L]]
		+	[[Category: Anti-oxidatve defense system]]
		+	[[Category: Covalent inhibitor]]
		+	[[Category: Oxidoreductase]]

OCA at 06:09, 26 September 2018

OCA — Wed, 26 Sep 2018 06:09:56 GMT

←Older revision		Revision as of 06:09, 26 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hkq is ON HOLD	+	The entry 6hkq is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6hkq" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2018 18:09:28 GMT

Protected "6hkq" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:09, 19 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hkq is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Sep 2018 18:09:27 GMT

New page: '''Unreleased structure''' The entry 6hkq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hkq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]