6hns - Revision history

OCA at 11:33, 24 January 2024

OCA — Wed, 24 Jan 2024 11:33:53 GMT

←Older revision		Revision as of 11:33, 24 January 2024
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	==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==		==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==
-	<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='6hns' size='340' side='right'caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_baa-920 Atcc baa-920~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrincola_lacisaponensis Nitrincola lacisaponensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HNS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADINL_0099 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267850 ATCC BAA-920])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [~~http~~://pdbe.org/6hns PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hns PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [https://pdbe.org/6hns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [https://www.ebi.ac.uk/pdbsum/6hns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A063Y6V3_9GAMM A0A063Y6V3_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6hns" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6hns" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc baa-920~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fiorentini, F~~]]	+	[[Category: Nitrincola lacisaponensis]]
-	[[Category: ~~Mattevi, A~~]]	+	[[Category: Fiorentini F]]
-	[[Category: ~~Drug metabolism]]~~	+	[[Category: Mattevi A]]
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Indigo]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Thermostability~~]]	+

OCA at 08:26, 6 March 2019

OCA — Wed, 06 Mar 2019 08:26:35 GMT

←Older revision		Revision as of 08:26, 6 March 2019
Line 1:		Line 1:

	==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==		==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==
-	<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-920 Atcc baa-920]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>		<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-920 Atcc baa-920]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>

OCA at 12:37, 16 January 2019

OCA — Wed, 16 Jan 2019 12:37:56 GMT

←Older revision		Revision as of 12:37, 16 January 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The flavin-containing monooxygenases (FMOs) play an important role in drug metabolism but they also have a high potential in industrial biotransformations. Among the hitherto characterized FMOs, there was no thermostable representative, while such biocatalyst would be valuable for FMO-based applications. Through a targeted genome mining approach, we have identified a gene encoding for a putative FMO from Nitrincola lacisaponensis, an alkaliphilic extremophile bacterium. Herein, we report the biochemical and structural characterization of this newly discovered bacterial FMO (NiFMO). NiFMO can be expressed as active and soluble enzyme at high level in Escherichia coli (90-100 mg/L of culture). NiFMO is relatively thermostable (melting temperature (Tm) of 51 degrees C), displays high organic solvent tolerance, and accepts a broad range of substrates. The crystal structure of NiFMO was solved at 1.8 A resolution, which allows future structure-based enzyme engineering. Altogether, NiFMO represents an interesting newly discovered enzyme with the appropriate features to develop into an industrially applied biocatalyst.
		+
		+	Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO).,Loncar N, Fiorentini F, Bailleul G, Savino S, Romero E, Mattevi A, Fraaije MW Appl Microbiol Biotechnol. 2019 Jan 4. pii: 10.1007/s00253-018-09579-w. doi:, 10.1007/s00253-018-09579-w. PMID:30607493<ref>PMID:30607493</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hns" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:47, 9 January 2019

OCA — Wed, 09 Jan 2019 06:47:43 GMT

←Older revision		Revision as of 06:47, 9 January 2019
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	<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-920 Atcc baa-920]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADINL_0099 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267850 ATCC BAA-920])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc baa-920]]
	[[Category: Fiorentini, F]]		[[Category: Fiorentini, F]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]

OCA at 06:10, 2 January 2019

OCA — Wed, 02 Jan 2019 06:10:23 GMT

←Older revision		Revision as of 06:10, 2 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6hns is ON HOLD~~	+	==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==
-		+	<StructureSection load='6hns' size='340' side='right' caption='[[6hns]], [[Resolution\|resolution]] 1.84Å' scene=''>
-	~~Authors: Fiorentini, F., Mattevi, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>
-	~~Description:~~ Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fiorentini, F]]		[[Category: Fiorentini, F]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]
		+	[[Category: Drug metabolism]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Indigo]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Thermostability]]

OCA: Protected "6hns" [edit=sysop:move=sysop]

OCA — Wed, 26 Sep 2018 06:13:31 GMT

Protected "6hns" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:13, 26 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hns is ON HOLD Authors: Fiorentini, F., Mattevi, A. Description: Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in c...

OCA — Wed, 26 Sep 2018 06:13:30 GMT

New page: '''Unreleased structure''' The entry 6hns is ON HOLD Authors: Fiorentini, F., Mattevi, A. Description: Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in c...

New page

'''Unreleased structure'''

The entry 6hns is ON HOLD

Authors: Fiorentini, F., Mattevi, A.

Description: Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+
[[Category: Unreleased Structures]]
[[Category: Fiorentini, F]]
[[Category: Mattevi, A]]