6hqg - Revision history

OCA at 11:37, 24 January 2024

OCA — Wed, 24 Jan 2024 11:37:15 GMT

←Older revision		Revision as of 11:37, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6hqg' size='340' side='right'caption='[[6hqg]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6hqg' size='340' side='right'caption='[[6hqg]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hqg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Phezh Phezh~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HQG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hqg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phenylobacterium_zucineum_HLK1 Phenylobacterium zucineum HLK1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HQG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~p450, PHZ_c0813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=450851 PHEZH])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqg OCA], [~~http~~://pdbe.org/6hqg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hqg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hqg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqg OCA], [https://pdbe.org/6hqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hqg RCSB], [https://www.ebi.ac.uk/pdbsum/6hqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B4RGA3_PHEZH B4RGA3_PHEZH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6hqg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6hqg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phezh~~]]	+	[[Category: Phenylobacterium zucineum HLK1]]
-	[[Category: Fiorentini, F]]	+	[[Category: Fiorentini F]]
-	[[Category: Mattevi, A]]	+	[[Category: Mattevi A]]
-	~~[[Category: Biocatalysis]]~~	+
-	~~[[Category: Cyp153 family]]~~	+
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 09:14, 1 May 2019

OCA — Wed, 01 May 2019 09:14:28 GMT

←Older revision		Revision as of 09:14, 1 May 2019
Line 1:		Line 1:

	==Cytochrome P450-153 from Phenylobacterium zucineum==		==Cytochrome P450-153 from Phenylobacterium zucineum==
-	<StructureSection load='6hqg' size='340' side='right' caption='[[6hqg]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='6hqg' size='340' side='right'caption='[[6hqg]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hqg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HQG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hqg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Phezh Phezh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HQG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">p450, PHZ_c0813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=450851 PHEZH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqg OCA], [http://pdbe.org/6hqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqg RCSB], [http://www.ebi.ac.uk/pdbsum/6hqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqg OCA], [http://pdbe.org/6hqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqg RCSB], [http://www.ebi.ac.uk/pdbsum/6hqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqg ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Phezh]]
	[[Category: Fiorentini, F]]		[[Category: Fiorentini, F]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]

OCA at 06:51, 12 December 2018

OCA — Wed, 12 Dec 2018 06:51:34 GMT

←Older revision		Revision as of 06:51, 12 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hqg is ~~ON HOLD~~	+	==Cytochrome P450-153 from Phenylobacterium zucineum==
		+	<StructureSection load='6hqg' size='340' side='right' caption='[[6hqg]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hqg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HQG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqg OCA], [http://pdbe.org/6hqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqg RCSB], [http://www.ebi.ac.uk/pdbsum/6hqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CYP153s are bacterial class I P450 enzymes traditionally described as alkane hydroxylases with a high terminal regioselectivity. They have been more recently shown to also catalyze hydroxylations at nonactivated carbon atoms of small heterocycles. The aim of our work was to perform an extensive characterization of this subfamily in order to deliver a toolbox of CYP153 enzymes for further development as biocatalysts. Through the screening of recently sequenced bacterial genomes, 20 CYP153s were selected, comprising 17 single monooxygenase domains and three multidomain variants, where the monooxygenase domain is naturally fused to its redox partners in a single polypeptide chain. The 20 novel variants were heterologously expressed, and their activity was screened toward octane and small heterocycles. A more extended substrate characterization was then performed on three representative candidates, and their crystal structures were unveiled and compared with those of the known CYP153A7 and CYP153A33. The tested enzymes displayed a wide range of activities, ranging from Omega and Omega-1 hydroxylations of lauric acid to indigo-generating indole modification. The comparative analysis highlighted a conserved architecture and amino acid composition of the catalytic core close to the heme, while showing a huge degree of structural plasticity and flexibility in those regions hosting the substrate recognition sites. Although dealing with this type of conformational variability adds a layer of complexity and difficulty to structure-based protein engineering, such diversity in substrate acceptance and recognition promotes the investigated CYP153s as a prime choice for tailoring designer hydroxylases.

-	~~Authors: Fiorentini~~, F., ~~Mattevi~~, A.	+	The Extreme Structural Plasticity in the CYP153 Subfamily of P450s Directs Development of Designer Hydroxylases.,Fiorentini F, Hatzl AM, Schmidt S, Savino S, Glieder A, Mattevi A Biochemistry. 2018 Nov 16. doi: 10.1021/acs.biochem.8b01052. PMID:30398864<ref>PMID:30398864</ref>

-	~~Description: Cytochrome P450-153 from Phenylobacterium zucineum~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6hqg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fiorentini, F]]		[[Category: Fiorentini, F]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]
		+	[[Category: Biocatalysis]]
		+	[[Category: Cyp153 family]]
		+	[[Category: Cytochrome p450]]
		+	[[Category: Heme]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6hqg" [edit=sysop:move=sysop]

OCA — Wed, 03 Oct 2018 06:15:30 GMT

Protected "6hqg" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 3 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hqg is ON HOLD Authors: Fiorentini, F., Mattevi, A. Description: Cytochrome P450-153 from Phenylobacterium zucineum [[Category: Unreleased Structu...

OCA — Wed, 03 Oct 2018 06:15:29 GMT

New page: '''Unreleased structure''' The entry 6hqg is ON HOLD Authors: Fiorentini, F., Mattevi, A. Description: Cytochrome P450-153 from Phenylobacterium zucineum [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 6hqg is ON HOLD

Authors: Fiorentini, F., Mattevi, A.

Description: Cytochrome P450-153 from Phenylobacterium zucineum
[[Category: Unreleased Structures]]
[[Category: Fiorentini, F]]
[[Category: Mattevi, A]]