6hqi - Revision history

OCA at 11:37, 24 January 2024

OCA — Wed, 24 Jan 2024 11:37:21 GMT

←Older revision		Revision as of 11:37, 24 January 2024
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	<StructureSection load='6hqi' size='340' side='right'caption='[[6hqi]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6hqi' size='340' side='right'caption='[[6hqi]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hqi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HQI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CU:COPPER+(II)+ION'>CU</scene~~>, ~~<scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Catechol_oxidase Catechol oxidase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.1 1.10.3.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqi OCA], [~~http~~://pdbe.org/6hqi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hqi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hqi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqi OCA], [https://pdbe.org/6hqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hqi RCSB], [https://www.ebi.ac.uk/pdbsum/6hqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PPOA_SOLLC PPOA_SOLLC] Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6hqi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6hqi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tyrosinase 3D structures\|Tyrosinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Catechol oxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bijelic, A~~]]	+	[[Category: Solanum lycopersicum]]
-	[[Category: ~~Kampatsikas, I]]~~	+	[[Category: Bijelic A]]
-	~~[[Category: Rompel,~~ A]]	+	[[Category: Kampatsikas I]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Rompel A]]
-	[[Category: ~~Polyphenol oxidase]]~~	+
-	~~[[Category: Tyrosinase~~]]	+

OCA at 07:56, 20 March 2019

OCA — Wed, 20 Mar 2019 07:56:07 GMT

←Older revision		Revision as of 07:56, 20 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hqi is ~~ON HOLD until Paper~~ Publication	+	==holo-form of polyphenol oxidase from Solanum lycopersicum==
		+	<StructureSection load='6hqi' size='340' side='right'caption='[[6hqi]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hqi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HQI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_oxidase Catechol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.1 1.10.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqi OCA], [http://pdbe.org/6hqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqi RCSB], [http://www.ebi.ac.uk/pdbsum/6hqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqi ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyphenol oxidases (PPOs) contain the structurally similar enzymes tyrosinases (TYRs) and catechol oxidases (COs). Two cDNAs encoding pro-PPOs from tomato (Solanum lycopersicum) were cloned and heterologously expressed in Escherichia coli. The two pro-PPOs (SlPPO1-2) differ remarkably in their activity as SlPPO1 reacts with the monophenols tyramine (kcat = 7.94 s(-1)) and phloretin (kcat = 2.42 s(-1)) and was thus characterized as TYR, whereas SlPPO2 accepts only diphenolic substrates like dopamine (kcat = 1.99 s(-1)) and caffeic acid (kcat = 20.33 s(-1)) rendering this enzyme a CO. This study, for the first time, characterizes a plant TYR and CO originating from the same organism. Moreover, X-ray structure analysis of the latent holo- and apo-SlPPO1 (PDB: 6HQI and 6HQJ) reveals an unprecedented high flexibility of the gatekeeper residue phenylalanine (Phe270). Docking studies showed that depending on its orientation the gatekeeper residue could either stabilize and correctly position incoming substrates or hinder their entrance into the active site. Furthermore, phloretin, a substrate of SIPPO1 (Km = 0.11 mM), is able to approach the active centre of SlPPO1 with both phenolic rings. Kinetic and structural results indicate that phloretin could act as a natural substrate and connote the participation of PPOs in flavonoid-biosynthesis.

-	~~Authors~~:	+	Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity.,Kampatsikas I, Bijelic A, Rompel A Sci Rep. 2019 Mar 11;9(1):4022. doi: 10.1038/s41598-019-39687-0. PMID:30858490<ref>PMID:30858490</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hqi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Catechol oxidase]]
		+	[[Category: Large Structures]]
		+	[[Category: Bijelic, A]]
		+	[[Category: Kampatsikas, I]]
		+	[[Category: Rompel, A]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Polyphenol oxidase]]
		+	[[Category: Tyrosinase]]

OCA: Protected "6hqi" [edit=sysop:move=sysop]

OCA — Wed, 03 Oct 2018 06:15:37 GMT

Protected "6hqi" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 3 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hqi is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Oct 2018 06:15:36 GMT

New page: '''Unreleased structure''' The entry 6hqi is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hqi is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]