6hri - Revision history

OCA at 12:42, 13 March 2019

OCA — Wed, 13 Mar 2019 12:42:22 GMT

←Older revision		Revision as of 12:42, 13 March 2019
Line 1:		Line 1:

	==Native YndL==		==Native YndL==
-	<StructureSection load='6hri' size='340' side='right' caption='[[6hri]], [[Resolution\|resolution]] 1.03Å' scene=''>	+	<StructureSection load='6hri' size='340' side='right'caption='[[6hri]], [[Resolution\|resolution]] 1.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hri]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hri]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5onk\|5onk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5onk\|5onk]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B4417_0239 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hri OCA], [http://pdbe.org/6hri PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hri RCSB], [http://www.ebi.ac.uk/pdbsum/6hri PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hri ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hri OCA], [http://pdbe.org/6hri PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hri RCSB], [http://www.ebi.ac.uk/pdbsum/6hri PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hri ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The identification of new strategies to fight bacterial infections in view of the spread of multiple resistance to antibiotics has become mandatory. It has been demonstrated that several bacteria develop poly-gamma-glutamic acid (gamma-PGA) capsules as a protection from external insults and/or host defence systems. Among the pathogens that shield themselves in these capsules are Bacillus anthracis, Francisella tularensis and several Staphylococcus strains. These are important pathogens with a profound influence on human health. The recently characterised gamma-PGA hydrolases, which can dismantle the gamma-PGA-capsules, are an attractive new direction that can offer real hope for the development of alternatives to antibiotics, particularly in cases of multidrug resistant bacteria. We have characterised in detail the cleaving mechanism and stereospecificity of the enzyme PghL (previously named YndL) from Bacillus subtilis encoded by a gene of phagic origin and dramatically efficient in degrading the long polymeric chains of gamma-PGA. We used X-ray crystallography to solve the three-dimensional structures of the enzyme in its zinc-free, zinc-bound and complexed forms. The protein crystallised with a gamma-PGA hexapeptide substrate and thus reveals details of the interaction which could explain the stereospecificity observed and give hints on the catalytic mechanism of this class of hydrolytic enzymes.
		+
		+	The structure of PghL hydrolase bound to its substrate poly-gamma-glutamate.,Ramaswamy S, Rasheed M, Morelli CF, Calvio C, Sutton BJ, Pastore A FEBS J. 2018 Dec;285(24):4575-4589. doi: 10.1111/febs.14688. Epub 2018 Nov 19. PMID:30387270<ref>PMID:30387270</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hri" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Calvio, C]]		[[Category: Calvio, C]]
	[[Category: Morelli, C]]		[[Category: Morelli, C]]

OCA at 08:16, 10 October 2018

OCA — Wed, 10 Oct 2018 08:16:45 GMT

←Older revision		Revision as of 08:16, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hri ~~is ON HOLD~~	+	==Native YndL==
-		+	<StructureSection load='6hri' size='340' side='right' caption='[[6hri]], [[Resolution\|resolution]] 1.03Å' scene=''>
-	~~Authors~~: ~~Ramaswamy, S~~., ~~Rasheed~~, M., ~~Morelli~~, C., ~~Calvio, C~~., ~~Sutton, B~~., ~~Pastore, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hri]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRI FirstGlance]. <br>
-	~~Description~~: ~~Native YndL~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5onk\|5onk]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hri OCA], [http://pdbe.org/6hri PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hri RCSB], [http://www.ebi.ac.uk/pdbsum/6hri PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hri ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Calvio, C]]
		+	[[Category: Morelli, C]]
		+	[[Category: Pastore, A]]
	[[Category: Ramaswamy, S]]		[[Category: Ramaswamy, S]]
	[[Category: Rasheed, M]]		[[Category: Rasheed, M]]
	[[Category: Sutton, B]]		[[Category: Sutton, B]]
-	[[Category: ~~Calvio, C~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~Morelli, C]]~~	+	[[Category: Pga hydrolase]]
-	~~[[Category: Pastore, A~~]]	+

OCA: Protected "6hri" [edit=sysop:move=sysop]

OCA — Wed, 03 Oct 2018 06:18:15 GMT

Protected "6hri" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:18, 3 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hri is ON HOLD Authors: Ramaswamy, S., Rasheed, M., Morelli, C., Calvio, C., Sutton, B., Pastore, A. Description: Native YndL [[Category: Unreleas...

OCA — Wed, 03 Oct 2018 06:18:13 GMT

New page: '''Unreleased structure''' The entry 6hri is ON HOLD Authors: Ramaswamy, S., Rasheed, M., Morelli, C., Calvio, C., Sutton, B., Pastore, A. Description: Native YndL [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 6hri is ON HOLD

Authors: Ramaswamy, S., Rasheed, M., Morelli, C., Calvio, C., Sutton, B., Pastore, A.

Description: Native YndL
[[Category: Unreleased Structures]]
[[Category: Ramaswamy, S]]
[[Category: Rasheed, M]]
[[Category: Sutton, B]]
[[Category: Calvio, C]]
[[Category: Morelli, C]]
[[Category: Pastore, A]]