6hrr - Revision history

OCA at 11:38, 24 January 2024

OCA — Wed, 24 Jan 2024 11:38:01 GMT

←Older revision		Revision as of 11:38, 24 January 2024
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	<StructureSection load='6hrr' size='340' side='right'caption='[[6hrr]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6hrr' size='340' side='right'caption='[[6hrr]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hrr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HRR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hrr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HRR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCOLN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hrr OCA], [~~http~~://pdbe.org/6hrr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hrr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hrr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hrr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hrr OCA], [https://pdbe.org/6hrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hrr RCSB], [https://www.ebi.ac.uk/pdbsum/6hrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hrr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MCLN2_HUMAN MCLN2_HUMAN]] Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19940139, PubMed:19885840). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (PubMed:17662026). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (By similarity). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (By similarity). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events (PubMed:19885840).[UniProtKB:Q8K595]<ref>PMID:17662026</ref> <ref>PMID:19885840</ref> <ref>PMID:19940139</ref>	+	[https://www.uniprot.org/uniprot/MCLN2_HUMAN MCLN2_HUMAN] Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19940139, PubMed:19885840). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (PubMed:17662026). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (By similarity). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (By similarity). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events (PubMed:19885840).[UniProtKB:Q8K595]<ref>PMID:17662026</ref> <ref>PMID:19885840</ref> <ref>PMID:19940139</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bader, N]]	+	[[Category: Bader N]]
-	[[Category: Hellmich~~, U A~~]]	+	[[Category: Hellmich UA]]
-	[[Category: Viet~~, K K~~]]	+	[[Category: Viet KK]]
-	[[Category: Wagner, A]]	+	[[Category: Wagner A]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Trpml channel mucolipin cation channel endolysosomal system~~]]	+

OCA at 17:21, 14 August 2019

OCA — Wed, 14 Aug 2019 17:21:13 GMT

←Older revision		Revision as of 17:21, 14 August 2019
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	<StructureSection load='6hrr' size='340' side='right'caption='[[6hrr]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6hrr' size='340' side='right'caption='[[6hrr]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hrr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hrr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCOLN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hrr OCA], [http://pdbe.org/6hrr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hrr RCSB], [http://www.ebi.ac.uk/pdbsum/6hrr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hrr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hrr OCA], [http://pdbe.org/6hrr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hrr RCSB], [http://www.ebi.ac.uk/pdbsum/6hrr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hrr ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bader, N]]		[[Category: Bader, N]]

OCA at 05:55, 19 June 2019

OCA — Wed, 19 Jun 2019 05:55:03 GMT

←Older revision		Revision as of 05:55, 19 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hrr is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of the TRPML2 ELD at pH 6.5==
		+	<StructureSection load='6hrr' size='340' side='right'caption='[[6hrr]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hrr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HRR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hrr OCA], [http://pdbe.org/6hrr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hrr RCSB], [http://www.ebi.ac.uk/pdbsum/6hrr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hrr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCLN2_HUMAN MCLN2_HUMAN]] Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19940139, PubMed:19885840). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (PubMed:17662026). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (By similarity). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (By similarity). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events (PubMed:19885840).[UniProtKB:Q8K595]<ref>PMID:17662026</ref> <ref>PMID:19885840</ref> <ref>PMID:19940139</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TRPML2 is the least structurally characterized mammalian transient receptor potential mucolipin ion channel. The TRPML family hallmark is a large extracytosolic/lumenal domain (ELD) between transmembrane helices S1 and S2. We present crystal structures of the tetrameric human TRPML2 ELD at pH 6.5 (2.0 A) and 4.5 (2.95 A), corresponding to the pH values in recycling endosomes and lysosomes. Isothermal titration calorimetry shows Ca(2+) binding to the highly acidic central pre-pore loop which is abrogated at low pH, in line with a pH-dependent channel regulation model. Small angle X-ray scattering confirms the ELD dimensions in solution. Changes in pH or Ca(2+) concentration do not affect the protein's secondary structure, but can influence ELD oligomer integrity according to native mass spectrometry. Our data thus complete the set of high-resolution views of human TRPML channel ELDs and reveal some structural responses to the conditions the TRPML2 ELD encounters as the channel traffics through the endolysosomal system.

-	~~Authors~~:	+	Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.,Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA Structure. 2019 May 14. pii: S0969-2126(19)30161-3. doi:, 10.1016/j.str.2019.04.016. PMID:31178222<ref>PMID:31178222</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hrr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bader, N]]
		+	[[Category: Hellmich, U A]]
		+	[[Category: Viet, K K]]
		+	[[Category: Wagner, A]]
		+	[[Category: Membrane protein]]
		+	[[Category: Trpml channel mucolipin cation channel endolysosomal system]]

OCA at 04:40, 24 April 2019

OCA — Wed, 24 Apr 2019 04:40:13 GMT

←Older revision		Revision as of 04:40, 24 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hrr is ON HOLD	+	The entry 6hrr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6hrr" [edit=sysop:move=sysop]

OCA — Wed, 10 Oct 2018 06:11:44 GMT

Protected "6hrr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:11, 10 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hrr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Oct 2018 06:11:43 GMT

New page: '''Unreleased structure''' The entry 6hrr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hrr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]