6hse - Revision history

OCA at 07:34, 1 May 2024

OCA — Wed, 01 May 2024 07:34:59 GMT

←Older revision		Revision as of 07:34, 1 May 2024
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	==Structure of dithionite-reduced RsrR in spacegroup P2(1)==		==Structure of dithionite-reduced RsrR in spacegroup P2(1)==
-	<StructureSection load='6hse' size='340' side='right' caption='[[6hse]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='6hse' size='340' side='right'caption='[[6hse]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hse]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strvp Strvp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hse]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae_ATCC_10712 Streptomyces venezuelae ATCC 10712]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HSE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hsd\|6hsd]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SVEN_6563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=953739 STRVP])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hse OCA], [https://pdbe.org/6hse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hse RCSB], [https://www.ebi.ac.uk/pdbsum/6hse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hse ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hse OCA], [~~http~~://pdbe.org/6hse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hse ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/F2RGC9_STRVP F2RGC9_STRVP]
-	~~The recently discovered Rrf2 family transcriptional regulator RsrR coordinates a~~ [2Fe-2S] cluster. Remarkably, binding of the protein to RsrR-regulated promoter DNA sequences is switched on and off through the facile cycling of the [2Fe-2S] cluster be-tween +2 and +1 states. Here, we report high resolution crystal structures of the RsrR dimer, revealing that the [2Fe-2S] cluster is asymmetrically coordinated across the RsrR monomer-monomer interface by two Cys residues from one subunit and His and Glu residues from the other. To our knowledge, this is the first example of a protein bound [Fe-S] cluster with three different amino acid side chains as ligands, and of Glu acting as ligand to a [2Fe-2S] cluster. Analyses of RsrR structures revealed a conformation-al change, centered on Trp9, which results in a significant shift in the DNA-binding helix-turn-helix region.	+
-		+
-	The Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu and His Residues.,Volbeda A, Pellicer Martinez MT, Crack JC, Amara P, Gigarel O, Munnoch JT, Hutchings MI, Darnault C, Le Brun NE, Fontecilla-Camps JC J Am Chem Soc. 2019 Jan 18. doi: ~~10.1021~~/~~jacs.8b10823. PMID:30657661<ref>PMID:30657661</ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6hse" style="background-color:#fffaf0;"><~~/~~div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Strvp~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fontecilla-Camps, J C~~]]	+	[[Category: Streptomyces venezuelae ATCC 10712]]
-	[[Category: ~~Volbeda, A~~]]	+	[[Category: Fontecilla-Camps JC]]
-	[[Category: ~~Iron sulfur cluster]]~~	+	[[Category: Volbeda A]]
-	~~[[Category: Redox sensor]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 09:52, 13 February 2019

OCA — Wed, 13 Feb 2019 09:52:04 GMT

←Older revision		Revision as of 09:52, 13 February 2019
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	<StructureSection load='6hse' size='340' side='right' caption='[[6hse]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6hse' size='340' side='right' caption='[[6hse]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hse]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hse]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strvp Strvp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hsd\|6hsd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hsd\|6hsd]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SVEN_6563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=953739 STRVP])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hse OCA], [http://pdbe.org/6hse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hse RCSB], [http://www.ebi.ac.uk/pdbsum/6hse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hse OCA], [http://pdbe.org/6hse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hse RCSB], [http://www.ebi.ac.uk/pdbsum/6hse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hse ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Strvp]]
	[[Category: Fontecilla-Camps, J C]]		[[Category: Fontecilla-Camps, J C]]
	[[Category: Volbeda, A]]		[[Category: Volbeda, A]]

OCA at 08:27, 30 January 2019

OCA — Wed, 30 Jan 2019 08:27:33 GMT

←Older revision		Revision as of 08:27, 30 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hse is ~~ON HOLD~~	+	==Structure of dithionite-reduced RsrR in spacegroup P2(1)==
		+	<StructureSection load='6hse' size='340' side='right' caption='[[6hse]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hse]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HSE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hsd\|6hsd]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hse OCA], [http://pdbe.org/6hse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hse RCSB], [http://www.ebi.ac.uk/pdbsum/6hse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hse ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recently discovered Rrf2 family transcriptional regulator RsrR coordinates a [2Fe-2S] cluster. Remarkably, binding of the protein to RsrR-regulated promoter DNA sequences is switched on and off through the facile cycling of the [2Fe-2S] cluster be-tween +2 and +1 states. Here, we report high resolution crystal structures of the RsrR dimer, revealing that the [2Fe-2S] cluster is asymmetrically coordinated across the RsrR monomer-monomer interface by two Cys residues from one subunit and His and Glu residues from the other. To our knowledge, this is the first example of a protein bound [Fe-S] cluster with three different amino acid side chains as ligands, and of Glu acting as ligand to a [2Fe-2S] cluster. Analyses of RsrR structures revealed a conformation-al change, centered on Trp9, which results in a significant shift in the DNA-binding helix-turn-helix region.

-	~~Authors~~:	+	The Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu and His Residues.,Volbeda A, Pellicer Martinez MT, Crack JC, Amara P, Gigarel O, Munnoch JT, Hutchings MI, Darnault C, Le Brun NE, Fontecilla-Camps JC J Am Chem Soc. 2019 Jan 18. doi: 10.1021/jacs.8b10823. PMID:30657661<ref>PMID:30657661</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6hse" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fontecilla-Camps, J C]]
		+	[[Category: Volbeda, A]]
		+	[[Category: Iron sulfur cluster]]
		+	[[Category: Redox sensor]]
		+	[[Category: Transcription]]

OCA: Protected "6hse" [edit=sysop:move=sysop]

OCA — Wed, 10 Oct 2018 06:13:27 GMT

Protected "6hse" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:13, 10 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hse is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Oct 2018 06:13:26 GMT

New page: '''Unreleased structure''' The entry 6hse is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hse is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]