6hto - Revision history

OCA at 11:39, 24 January 2024

OCA — Wed, 24 Jan 2024 11:39:20 GMT

←Older revision		Revision as of 11:39, 24 January 2024
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	<StructureSection load='6hto' size='340' side='right'caption='[[6hto]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='6hto' size='340' side='right'caption='[[6hto]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hto]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"streptomyces_actuosus"_pinnert_et_al. "streptomyces~~ actuosus~~" pinnert et al.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HTO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hto]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_actuosus Streptomyces actuosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HTO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6htk\|6htk]], [[6htm\|6htm]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nosL, nocL, DMT42_23170 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1885 "Streptomyces actuosus" Pinnert et al.])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hto OCA], [https://pdbe.org/6hto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hto RCSB], [https://www.ebi.ac.uk/pdbsum/6hto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hto ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hto OCA], [~~http~~://pdbe.org/6hto PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hto RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hto PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hto ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C6FX51_STRAS C6FX51_STRAS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces actuosus pinnert et al]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amara, P]]	+	[[Category: Streptomyces actuosus]]
-	[[Category: Bella, M]]	+	[[Category: Amara P]]
-	[[Category: Gambarelli, S]]	+	[[Category: Bella M]]
-	[[Category: Martin, L]]	+	[[Category: Gambarelli S]]
-	[[Category: Mouesca~~, J M~~]]	+	[[Category: Martin L]]
-	[[Category: Nicolet, Y]]	+	[[Category: Mouesca JM]]
-	[[Category: Saragaglia, C]]	+	[[Category: Nicolet Y]]
-	~~[[Category: Antibiotic]]~~	+	[[Category: Saragaglia C]]
-	~~[[Category: Nosl]]~~	+
-	~~[[Category: Radical sam~~]]	+

OCA at 07:59, 24 April 2019

OCA — Wed, 24 Apr 2019 07:59:41 GMT

←Older revision		Revision as of 07:59, 24 April 2019
Line 1:		Line 1:

	==Tryptophan lyase 'empty state'==		==Tryptophan lyase 'empty state'==
-	<StructureSection load='6hto' size='340' side='right' caption='[[6hto]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='6hto' size='340' side='right'caption='[[6hto]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6hto]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTO FirstGlance]. <br>		<table><tr><td colspan='2'>[[6hto]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTO FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces actuosus pinnert et al]]		[[Category: Streptomyces actuosus pinnert et al]]
		+	[[Category: Large Structures]]
	[[Category: Amara, P]]		[[Category: Amara, P]]
	[[Category: Bella, M]]		[[Category: Bella, M]]

OCA at 21:07, 2 December 2018

OCA — Sun, 02 Dec 2018 21:07:24 GMT

←Older revision		Revision as of 21:07, 2 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6hto is ~~ON HOLD until Paper~~ Publication	+	==Tryptophan lyase 'empty state'==
		+	<StructureSection load='6hto' size='340' side='right' caption='[[6hto]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hto]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6htk\|6htk]], [[6htm\|6htm]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nosL, nocL, DMT42_23170 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1885 "Streptomyces actuosus" Pinnert et al.])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hto OCA], [http://pdbe.org/6hto PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hto RCSB], [http://www.ebi.ac.uk/pdbsum/6hto PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hto ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The radical S-adenosyl-l-methionine tryptophan lyase uses radical-based chemistry to convert l-tryptophan into 3-methyl-2-indolic acid, a fragment in the biosynthesis of the thiopeptide antibiotic nosiheptide. This complex reaction involves several successive steps corresponding to (i) the activation by a specific hydrogen-atom abstraction, (ii) an unprecedented CO2(-) radical migration, (iii) a cyanide fragment release, and (iv) the termination of the radical-based reaction. In vitro study of this reaction is made more difficult because the enzyme produces a significant amount of a shunt product instead of the natural product. Here, using a combination of X-ray crystallography, electron paramagnetic resonance spectroscopy, and quantum and hybrid quantum mechanical/molecular mechanical calculations, we have deciphered the fine mechanism of the key CO2(-) radical migration, highlighting how the preorganized active site of the protein tightly controls this reaction.

-	~~Authors~~: Amara, P., Mouesca~~, J.M.~~, Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y.	+	Radical S-Adenosyl-l-methionine Tryptophan Lyase (NosL): How the Protein Controls the Carboxyl Radical *CO2(-) Migration.,Amara P, Mouesca JM, Bella M, Martin L, Saragaglia C, Gambarelli S, Nicolet Y J Am Chem Soc. 2018 Nov 16. doi: 10.1021/jacs.8b09142. PMID:30418774<ref>PMID:30418774</ref>

-	~~Description~~: ~~Tryptophan lyase 'empty state'~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Martin~~, L]]	+	<div class="pdbe-citations 6hto" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Streptomyces actuosus pinnert et al]]
		+	[[Category: Amara, P]]
	[[Category: Bella, M]]		[[Category: Bella, M]]
	[[Category: Gambarelli, S]]		[[Category: Gambarelli, S]]
		+	[[Category: Martin, L]]
		+	[[Category: Mouesca, J M]]
	[[Category: Nicolet, Y]]		[[Category: Nicolet, Y]]
-	[[Category: Amara, P]]
	[[Category: Saragaglia, C]]		[[Category: Saragaglia, C]]
-	[[Category: ~~Mouesca, J.M~~]]	+	[[Category: Antibiotic]]
		+	[[Category: Nosl]]
		+	[[Category: Radical sam]]

OCA at 06:13, 17 October 2018

OCA — Wed, 17 Oct 2018 06:13:29 GMT

←Older revision		Revision as of 06:13, 17 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hto is ON HOLD	+	The entry 6hto is ON HOLD until Paper Publication

	Authors: Amara, P., Mouesca, J.M., Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y.		Authors: Amara, P., Mouesca, J.M., Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y.

-	Description: Tryptophan lyase 'empty state	+	Description: Tryptophan lyase 'empty state'
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Martin, L]]		[[Category: Martin, L]]

OCA: Protected "6hto" [edit=sysop:move=sysop]

OCA — Wed, 10 Oct 2018 06:16:54 GMT

Protected "6hto" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:16, 10 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hto is ON HOLD Authors: Amara, P., Mouesca, J.M., Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y. Description: Tryptophan lyase...

OCA — Wed, 10 Oct 2018 06:16:53 GMT

New page: '''Unreleased structure''' The entry 6hto is ON HOLD Authors: Amara, P., Mouesca, J.M., Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y. Description: Tryptophan lyase...

New page

'''Unreleased structure'''

The entry 6hto is ON HOLD

Authors: Amara, P., Mouesca, J.M., Bella, M., Martin, L., Saragaglia, C., Gambarelli, S., Nicolet, Y.

Description: Tryptophan lyase 'empty state
[[Category: Unreleased Structures]]
[[Category: Martin, L]]
[[Category: Bella, M]]
[[Category: Gambarelli, S]]
[[Category: Nicolet, Y]]
[[Category: Amara, P]]
[[Category: Saragaglia, C]]
[[Category: Mouesca, J.M]]