OCA at 15:25, 28 August 2019

OCA — Wed, 28 Aug 2019 15:25:43 GMT

←Older revision		Revision as of 15:25, 28 August 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6hyu is ~~ON HOLD~~	+	==Crystal structure of DHX8 helicase bound to single stranded poly-adenine RNA==
		+	<StructureSection load='6hyu' size='340' side='right'caption='[[6hyu]], [[Resolution\|resolution]] 3.22Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hyu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HYU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hyu OCA], [http://pdbe.org/6hyu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hyu RCSB], [http://www.ebi.ac.uk/pdbsum/6hyu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hyu ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DHX8_HUMAN DHX8_HUMAN]] Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.<ref>PMID:8608946</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DHX8 is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the spliceosome. Here we report the biochemical characterization of full-length human DHX8 and the catalytically active helicase core DHX8Delta547, alongside crystal structures of DHX8Delta547 bound to ADP and a structure of DHX8Delta547 bound to poly(A)6 single-strand RNA. Our results reveal that DHX8 has an in vitro binding preference for adenine-rich RNA and that RNA binding triggers release of ADP through significant conformational flexibility in the conserved DEAH-, P-loop and hook-turn motifs. We demonstrate the importance of R620 and both the hook-turn and hook-loop regions for DHX8 helicase activity, and propose that the hook-turn acts as a gatekeeper to regulate directional movement of the 3' end of RNA through the RNA binding channel. This study provides an in-depth understanding of the activity of DHX8 and contributes insights into the RNA unwinding mechanisms of the DEAH-box helicase family.

-	~~Authors:~~ Felisberto-Rodrigues, C., Thomas~~, J.C.~~, McAndrew~~, P.~~C., Le Bihan~~, Y.V.~~, Burke, R., Workman, P., van Montfort~~, R~~.L.M.	+	Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release.,Felisberto-Rodrigues C, Thomas JC, McAndrew C, Le Bihan YV, Burke R, Workman P, van Montfort RLM Biochem J. 2019 Aug 13. pii: BCJ20190383. doi: 10.1042/BCJ20190383. PMID:31409651<ref>PMID:31409651</ref>

-	~~Description: Crystal structure~~ of ~~DHX8 helicase bound to single stranded poly~~-~~adenine RNA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hyu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: RNA helicase]]
		+	[[Category: Bihan, Y V.Le]]
	[[Category: Burke, R]]		[[Category: Burke, R]]
	[[Category: Felisberto-Rodrigues, C]]		[[Category: Felisberto-Rodrigues, C]]
-	[[Category: ~~Van~~ Montfort, R.L.M]]	+	[[Category: McAndrew, P C]]
		+	[[Category: Montfort, R L.M van]]
		+	[[Category: Thomas, J C]]
	[[Category: Workman, P]]		[[Category: Workman, P]]
-	[[Category: ~~Le Bihan, Y.V~~]]	+	[[Category: Helicase]]
-	[[Category: ~~Mcandrew, P.C~~]]	+	[[Category: Rna]]
-	[[Category: ~~Thomas, J.C~~]]	+	[[Category: Rna binding protein]]
		+	[[Category: Splicing]]

OCA: Protected "6hyu" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2018 05:12:04 GMT

Protected "6hyu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 31 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hyu is ON HOLD Authors: Felisberto-Rodrigues, C., Thomas, J.C., McAndrew, P.C., Le Bihan, Y.V., Burke, R., Workman, P., van Montfort, R.L.M. Descr...

OCA — Wed, 31 Oct 2018 05:12:03 GMT

New page: '''Unreleased structure''' The entry 6hyu is ON HOLD Authors: Felisberto-Rodrigues, C., Thomas, J.C., McAndrew, P.C., Le Bihan, Y.V., Burke, R., Workman, P., van Montfort, R.L.M. Descr...

New page

'''Unreleased structure'''

The entry 6hyu is ON HOLD

Authors: Felisberto-Rodrigues, C., Thomas, J.C., McAndrew, P.C., Le Bihan, Y.V., Burke, R., Workman, P., van Montfort, R.L.M.

Description: Crystal structure of DHX8 helicase bound to single stranded poly-adenine RNA
[[Category: Unreleased Structures]]
[[Category: Burke, R]]
[[Category: Felisberto-Rodrigues, C]]
[[Category: Van Montfort, R.L.M]]
[[Category: Workman, P]]
[[Category: Le Bihan, Y.V]]
[[Category: Mcandrew, P.C]]
[[Category: Thomas, J.C]]

6hyu - Revision history

OCA at 15:25, 28 August 2019

OCA: Protected "6hyu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6hyu is ON HOLD Authors: Felisberto-Rodrigues, C., Thomas, J.C., McAndrew, P.C., Le Bihan, Y.V., Burke, R., Workman, P., van Montfort, R.L.M. Descr...