6i0t - Revision history

OCA at 11:43, 24 January 2024

OCA — Wed, 24 Jan 2024 11:43:57 GMT

←Older revision		Revision as of 11:43, 24 January 2024
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	==Crystal structure of DmTailor in complex with GpU==		==Crystal structure of DmTailor in complex with GpU==
-	<StructureSection load='6i0t' size='340' side='right' caption='[[6i0t]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6i0t' size='340' side='right'caption='[[6i0t]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i0t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I0T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I0T FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6i0s\|6i0s]]~~, [[~~6i0u~~\|~~6i0u~~]]~~, [[6i0v\|6i0v]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2~~.7.7.52 2.7.7.52] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0t OCA], [https://pdbe.org/6i0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i0t RCSB], [https://www.ebi.ac.uk/pdbsum/6i0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0t ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0t OCA], [~~http~~://pdbe.org/6i0t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6i0t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6i0t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0t ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME]] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>	+	[https://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6i0t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6i0t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA uridylyltransferase\|RNA uridylyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~RNA uridylyltransferase~~]]	+	[[Category: Drosophila melanogaster]]
-	[[Category: ~~Ivascu, A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Jinek, M]]~~	+	[[Category: Ivascu A]]
-	~~[[Category: Kroupova,~~ A]]	+	[[Category: Jinek M]]
-	[[Category: ~~Nucleotidyl transferase~~]]	+	[[Category: Kroupova A]]
-	[[Category: ~~Terminal uridyl transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:47, 5 December 2018

OCA — Wed, 05 Dec 2018 06:47:45 GMT

←Older revision		Revision as of 06:47, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6i0t is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of DmTailor in complex with GpU==
		+	<StructureSection load='6i0t' size='340' side='right' caption='[[6i0t]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i0t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I0T FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i0s\|6i0s]], [[6i0u\|6i0u]], [[6i0v\|6i0v]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0t OCA], [http://pdbe.org/6i0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i0t RCSB], [http://www.ebi.ac.uk/pdbsum/6i0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0t ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME]] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Non-templated 3'-uridylation of RNAs has emerged as an important mechanism for regulating the processing, stability and biological function of eukaryotic transcripts. In Drosophila, oligouridine tailing by the terminal uridylyl transferase (TUTase) Tailor of numerous RNAs induces their degradation by the exonuclease Dis3L2, which serves functional roles in RNA surveillance and mirtron RNA biogenesis. Tailor preferentially uridylates RNAs terminating in guanosine or uridine nucleotides but the structural basis underpinning its RNA substrate selectivity is unknown. Here, we report crystal structures of Tailor bound to a donor substrate analog or mono- and oligouridylated RNA products. These structures reveal specific amino acid residues involved in donor and acceptor substrate recognition, and complementary biochemical assays confirm the critical role of an active site arginine in conferring selectivity toward 3'-guanosine terminated RNAs. Notably, conservation of these active site features suggests that other eukaryotic TUTases, including mammalian TUT4 and TUT7, might exhibit similar, hitherto unknown, substrate selectivity. Together, these studies provide critical insights into the specificity of 3'-uridylation in eukaryotic post-transcriptional gene regulation.

-	~~Authors: Kroupova~~, A., Ivascu, A., ~~Jinek~~, M.	+	Structural basis for acceptor RNA substrate selectivity of the 3' terminal uridylyl transferase Tailor.,Kroupova A, Ivascu A, Reimao-Pinto MM, Ameres SL, Jinek M Nucleic Acids Res. 2018 Nov 20. pii: 5193338. doi: 10.1093/nar/gky1164. PMID:30462292<ref>PMID:30462292</ref>

-	~~Description: Crystal structure~~ of ~~DmTailor in complex with GpU~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Jinek, M~~]]	+	<div class="pdbe-citations 6i0t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA uridylyltransferase]]
	[[Category: Ivascu, A]]		[[Category: Ivascu, A]]
		+	[[Category: Jinek, M]]
	[[Category: Kroupova, A]]		[[Category: Kroupova, A]]
		+	[[Category: Nucleotidyl transferase]]
		+	[[Category: Terminal uridyl transferase]]
		+	[[Category: Transferase]]

OCA: Protected "6i0t" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:34:42 GMT

Protected "6i0t" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:34, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6i0t is ON HOLD until Paper Publication Authors: Kroupova, A., Ivascu, A., Jinek, M. Description: Crystal structure of DmTailor in complex with GpU...

OCA — Wed, 07 Nov 2018 10:34:40 GMT

New page: '''Unreleased structure''' The entry 6i0t is ON HOLD until Paper Publication Authors: Kroupova, A., Ivascu, A., Jinek, M. Description: Crystal structure of DmTailor in complex with GpU...

New page

'''Unreleased structure'''

The entry 6i0t is ON HOLD until Paper Publication

Authors: Kroupova, A., Ivascu, A., Jinek, M.

Description: Crystal structure of DmTailor in complex with GpU
[[Category: Unreleased Structures]]
[[Category: Jinek, M]]
[[Category: Ivascu, A]]
[[Category: Kroupova, A]]