6i0u - Revision history

OCA at 11:44, 24 January 2024

OCA — Wed, 24 Jan 2024 11:44:01 GMT

←Older revision		Revision as of 11:44, 24 January 2024
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	==Crystal structure of DmTailor in complex with U6 RNA==		==Crystal structure of DmTailor in complex with U6 RNA==
-	<StructureSection load='6i0u' size='340' side='right' caption='[[6i0u]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6i0u' size='340' side='right'caption='[[6i0u]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i0u]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I0U FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.001Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i0s\|6i0s]]~~, [[~~6i0t~~\|~~6i0t]], [[6i0v\|6i0v~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Tailor, CG1091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~7227 DROME])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0u OCA], [https://pdbe.org/6i0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i0u RCSB], [https://www.ebi.ac.uk/pdbsum/6i0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0u ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0u OCA], [~~http~~://pdbe.org/6i0u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6i0u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6i0u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0u ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME]] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>	+	[https://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6i0u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6i0u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA uridylyltransferase\|RNA uridylyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
-	[[Category: ~~RNA uridylyltransferase~~]]	+	[[Category: Large Structures]]
-	[[Category: Ivascu, A]]	+	[[Category: Ivascu A]]
-	[[Category: Jinek, M]]	+	[[Category: Jinek M]]
-	[[Category: Kroupova, A]]	+	[[Category: Kroupova A]]
-	~~[[Category: Nucleotidyl transferase]]~~	+
-	~~[[Category: Terminal uridyl transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:02, 19 December 2018

OCA — Wed, 19 Dec 2018 09:02:43 GMT

←Older revision		Revision as of 09:02, 19 December 2018
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	<StructureSection load='6i0u' size='340' side='right' caption='[[6i0u]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6i0u' size='340' side='right' caption='[[6i0u]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i0u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i0u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I0U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[~~d_1200012449~~\|~~d_1200012449~~]], [[~~d_1200012584~~\|~~d_1200012584~~]], [[~~d_1200012595~~\|~~d_1200012595~~]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i0s\|6i0s]], [[6i0t\|6i0t]], [[6i0v\|6i0v]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tailor, CG1091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0u OCA], [http://pdbe.org/6i0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i0u RCSB], [http://www.ebi.ac.uk/pdbsum/6i0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0u OCA], [http://pdbe.org/6i0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i0u RCSB], [http://www.ebi.ac.uk/pdbsum/6i0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0u ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
	[[Category: RNA uridylyltransferase]]		[[Category: RNA uridylyltransferase]]
	[[Category: Ivascu, A]]		[[Category: Ivascu, A]]

OCA at 06:47, 5 December 2018

OCA — Wed, 05 Dec 2018 06:47:46 GMT

←Older revision		Revision as of 06:47, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6i0u is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of DmTailor in complex with U6 RNA==
		+	<StructureSection load='6i0u' size='340' side='right' caption='[[6i0u]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i0u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I0U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[d_1200012449\|d_1200012449]], [[d_1200012584\|d_1200012584]], [[d_1200012595\|d_1200012595]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i0u OCA], [http://pdbe.org/6i0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i0u RCSB], [http://www.ebi.ac.uk/pdbsum/6i0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i0u ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TUTT_DROME TUTT_DROME]] Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis.<ref>PMID:26145174</ref> <ref>PMID:26145176</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Non-templated 3'-uridylation of RNAs has emerged as an important mechanism for regulating the processing, stability and biological function of eukaryotic transcripts. In Drosophila, oligouridine tailing by the terminal uridylyl transferase (TUTase) Tailor of numerous RNAs induces their degradation by the exonuclease Dis3L2, which serves functional roles in RNA surveillance and mirtron RNA biogenesis. Tailor preferentially uridylates RNAs terminating in guanosine or uridine nucleotides but the structural basis underpinning its RNA substrate selectivity is unknown. Here, we report crystal structures of Tailor bound to a donor substrate analog or mono- and oligouridylated RNA products. These structures reveal specific amino acid residues involved in donor and acceptor substrate recognition, and complementary biochemical assays confirm the critical role of an active site arginine in conferring selectivity toward 3'-guanosine terminated RNAs. Notably, conservation of these active site features suggests that other eukaryotic TUTases, including mammalian TUT4 and TUT7, might exhibit similar, hitherto unknown, substrate selectivity. Together, these studies provide critical insights into the specificity of 3'-uridylation in eukaryotic post-transcriptional gene regulation.

-	~~Authors: Kroupova~~, A., Ivascu, A., ~~Jinek~~, M.	+	Structural basis for acceptor RNA substrate selectivity of the 3' terminal uridylyl transferase Tailor.,Kroupova A, Ivascu A, Reimao-Pinto MM, Ameres SL, Jinek M Nucleic Acids Res. 2018 Nov 20. pii: 5193338. doi: 10.1093/nar/gky1164. PMID:30462292<ref>PMID:30462292</ref>

-	~~Description: Crystal structure~~ of ~~DmTailor in complex with U6 RNA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Jinek, M~~]]	+	<div class="pdbe-citations 6i0u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA uridylyltransferase]]
	[[Category: Ivascu, A]]		[[Category: Ivascu, A]]
		+	[[Category: Jinek, M]]
	[[Category: Kroupova, A]]		[[Category: Kroupova, A]]
		+	[[Category: Nucleotidyl transferase]]
		+	[[Category: Terminal uridyl transferase]]
		+	[[Category: Transferase]]

OCA: Protected "6i0u" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:34:44 GMT

Protected "6i0u" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:34, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6i0u is ON HOLD until Paper Publication Authors: Kroupova, A., Ivascu, A., Jinek, M. Description: Crystal structure of DmTailor in complex with U6 ...

OCA — Wed, 07 Nov 2018 10:34:43 GMT

New page: '''Unreleased structure''' The entry 6i0u is ON HOLD until Paper Publication Authors: Kroupova, A., Ivascu, A., Jinek, M. Description: Crystal structure of DmTailor in complex with U6 ...

New page

'''Unreleased structure'''

The entry 6i0u is ON HOLD until Paper Publication

Authors: Kroupova, A., Ivascu, A., Jinek, M.

Description: Crystal structure of DmTailor in complex with U6 RNA
[[Category: Unreleased Structures]]
[[Category: Jinek, M]]
[[Category: Ivascu, A]]
[[Category: Kroupova, A]]