6i1y - Revision history

OCA at 01:36, 11 April 2020

OCA — Sat, 11 Apr 2020 01:36:53 GMT

←Older revision		Revision as of 01:36, 11 April 2020
Line 3:		Line 3:
	<SX load='6i1y' size='340' side='right' viewer='molstar' caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6i1y' size='340' side='right' viewer='molstar' caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JS86_21435 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JS86_21435 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1y OCA], [http://pdbe.org/6i1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1y RCSB], [http://www.ebi.ac.uk/pdbsum/6i1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1y OCA], [http://pdbe.org/6i1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1y RCSB], [http://www.ebi.ac.uk/pdbsum/6i1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:59, 6 March 2020

OCA — Fri, 06 Mar 2020 21:59:22 GMT

←Older revision		Revision as of 21:59, 6 March 2020
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	==Vibrio vulnificus EpsD==		==Vibrio vulnificus EpsD==
-	<~~StructureSection~~ load='6i1y' size='340' side='right'caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<SX load='6i1y' size='340' side='right' viewer='molstar' caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Atcc 27562]]		[[Category: Atcc 27562]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:54, 31 July 2019

OCA — Wed, 31 Jul 2019 06:54:34 GMT

←Older revision		Revision as of 06:54, 31 July 2019
Line 3:		Line 3:
	<StructureSection load='6i1y' size='340' side='right'caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='6i1y' size='340' side='right'caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1y OCA], [http://pdbe.org/6i1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1y RCSB], [http://www.ebi.ac.uk/pdbsum/6i1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1y ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JS86_21435 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1y OCA], [http://pdbe.org/6i1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1y RCSB], [http://www.ebi.ac.uk/pdbsum/6i1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1y ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The type II secretion system (T2SS) is a cell envelope-spanning macromolecular complex that is prevalent in Gram-negative bacterial species. It serves as the predominant virulence mechanism of many bacteria including those of the emerging human pathogens Vibrio vulnificus and Aeromonas hydrophila. The system is composed of a core set of highly conserved proteins that assemble an inner membrane platform, a periplasmic pseudopilus and an outer membrane complex termed the secretin. Localization and assembly of secretins in the outer membrane requires recognition of secretin monomers by two different partner systems: an inner membrane accessory complex or a highly sequence-diverse outer membrane lipoprotein, termed the pilotin. In this study, we addressed the question of differential secretin assembly mechanisms by using cryo-electron microscopy to determine the structures of the secretins from A. hydrophila (pilotin-independent ExeD) and V. vulnificus (pilotin-dependent EpsD). These structures, at approximately 3.5 A resolution, reveal pentadecameric stoichiometries and C-terminal regions that carry a signature motif in the case of a pilotin-dependent assembly mechanism. We solved the crystal structure of the V. vulnificus EpsS pilotin and confirmed the importance of the signature motif for pilotin-dependent secretin assembly by performing modelling with the C-terminus of EpsD. We also show that secretin assembly is essential for membrane integrity and toxin secretion in V. vulnificus and establish that EpsD requires the coordinated activity of both the accessory complex EpsAB and the pilotin EpsS for full assembly and T2SS function. In contrast, mutation of the region of the S-domain that is normally the site of pilotin interactions has little effect on assembly or function of the ExeD secretin. Since secretins are essential outer membrane channels present in a variety of secretion systems, these results provide a structural and functional basis for understanding the key assembly steps for different members of this vast pore-forming family of proteins.
		+
		+	Structure and assembly of pilotin-dependent and -independent secretins of the type II secretion system.,Howard SP, Estrozi LF, Bertrand Q, Contreras-Martel C, Strozen T, Job V, Martins A, Fenel D, Schoehn G, Dessen A PLoS Pathog. 2019 May 13;15(5):e1007731. doi: 10.1371/journal.ppat.1007731., eCollection 2019 May. PMID:31083688<ref>PMID:31083688</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6i1y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 27562]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Contreras-Martel, C]]		[[Category: Contreras-Martel, C]]
	[[Category: Estrozi, L Farias]]		[[Category: Estrozi, L Farias]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 07:21, 10 April 2019

OCA — Wed, 10 Apr 2019 07:21:28 GMT

←Older revision		Revision as of 07:21, 10 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6i1y ~~is ON HOLD~~	+	==Vibrio vulnificus EpsD==
-		+	<StructureSection load='6i1y' size='340' side='right'caption='[[6i1y]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~Authors~~: ~~Contreras~~-~~Martel~~, C., ~~Farias Estrozi~~, L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6i1y]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1Y FirstGlance]. <br>
-	~~Description: Vibrio vulnificus EpsD~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1y OCA], [http://pdbe.org/6i1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1y RCSB], [http://www.ebi.ac.uk/pdbsum/6i1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1y ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Contreras-Martel, C]]		[[Category: Contreras-Martel, C]]
-	[[Category: ~~Farias~~ Estrozi, L]]	+	[[Category: Estrozi, L Farias]]
		+	[[Category: Protein transport]]

OCA: Protected "6i1y" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:38:20 GMT

Protected "6i1y" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:38, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6i1y is ON HOLD Authors: Contreras-Martel, C., Farias Estrozi, L. Description: Vibrio vulnificus EpsD Category: Unreleased Structures [[Categor...

OCA — Wed, 07 Nov 2018 10:38:18 GMT

New page: '''Unreleased structure''' The entry 6i1y is ON HOLD Authors: Contreras-Martel, C., Farias Estrozi, L. Description: Vibrio vulnificus EpsD Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 6i1y is ON HOLD

Authors: Contreras-Martel, C., Farias Estrozi, L.

Description: Vibrio vulnificus EpsD
[[Category: Unreleased Structures]]
[[Category: Contreras-Martel, C]]
[[Category: Farias Estrozi, L]]