6i2c - Revision history

OCA at 10:16, 15 February 2023

OCA — Wed, 15 Feb 2023 10:16:39 GMT

←Older revision		Revision as of 10:16, 15 February 2023
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	<StructureSection load='6i2c' size='340' side='right'caption='[[6i2c]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='6i2c' size='340' side='right'caption='[[6i2c]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i2c]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cho_cell_lines Cho cell lines~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I2C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6ers 6ers]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I2C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M77:5-(1,4-DIAZEPAN-1-SULFONYL)ISOQUINOLINE'>M77</scene>, <scene name='pdbligand=RIP:RIBOSE(PYRANOSE+FORM)'>RIP</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M77:5-(1,4-DIAZEPAN-1-SULFONYL)ISOQUINOLINE'>M77</scene>, <scene name='pdbligand=RIP:RIBOSE(PYRANOSE+FORM)'>RIP</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2c OCA], [https://pdbe.org/6i2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i2c RCSB], [https://www.ebi.ac.uk/pdbsum/6i2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2c ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRKACA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2c OCA], [~~http~~://pdbe.org/6i2c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6i2c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6i2c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2c ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~KAPCA_CRIGR KAPCA_CRIGR~~]] Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity). Phosphorylates APOBEC3G and AICDA (By similarity).	+	[https://www.uniprot.org/uniprot/G3HK48_CRIGR G3HK48_CRIGR]
		+
		+	==See Also==
		+	*[[CAMP-dependent protein kinase 3D structures\|CAMP-dependent protein kinase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cho cell lines~~]]	+	[[Category: Cricetulus griseus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: CAMP-dependent protein kinase]]~~	+	[[Category: Heine A]]
-	[[Category: Heine, A]]	+	[[Category: Klebe G]]
-	[[Category: Klebe, G]]	+	[[Category: Mueller JM]]
-	[[Category: Mueller~~, J M]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Peptidic ligand]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:26, 21 May 2019

OCA — Tue, 21 May 2019 07:26:34 GMT

←Older revision		Revision as of 07:26, 21 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6i2c is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of the Protein-Kinase A catalytic subunit from Cricetulus Griseus in complex with compounds RKp182 and Fasudil==
-		+	<StructureSection load='6i2c' size='340' side='right'caption='[[6i2c]], [[Resolution\|resolution]] 1.82Å' scene=''>
-	~~Authors~~: ~~Mueller~~, J.M., ~~Heine~~, A., ~~Klebe~~, G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6i2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2C FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure~~ of the ~~Protein~~-~~Kinase A catalytic subunit~~ from ~~Cricetulus Griseus~~ in complex with ~~compounds RKp182~~ and ~~Fasudil~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M77:5-(1,4-DIAZEPAN-1-SULFONYL)ISOQUINOLINE'>M77</scene>, <scene name='pdbligand=RIP:RIBOSE(PYRANOSE+FORM)'>RIP</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRKACA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2c OCA], [http://pdbe.org/6i2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2c RCSB], [http://www.ebi.ac.uk/pdbsum/6i2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2c ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAPCA_CRIGR KAPCA_CRIGR]] Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity). Phosphorylates APOBEC3G and AICDA (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cho cell lines]]
		+	[[Category: Large Structures]]
		+	[[Category: CAMP-dependent protein kinase]]
	[[Category: Heine, A]]		[[Category: Heine, A]]
-	[[Category: Mueller, J.M]]
	[[Category: Klebe, G]]		[[Category: Klebe, G]]
		+	[[Category: Mueller, J M]]
		+	[[Category: Complex]]
		+	[[Category: Peptidic ligand]]
		+	[[Category: Transferase]]

OCA: Protected "6i2c" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:39:27 GMT

Protected "6i2c" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:39, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6i2c is ON HOLD until Paper Publication Authors: Mueller, J.M., Heine, A., Klebe, G. Description: Crystal Structure of the Protein-Kinase A catalyt...

OCA — Wed, 07 Nov 2018 10:39:26 GMT

New page: '''Unreleased structure''' The entry 6i2c is ON HOLD until Paper Publication Authors: Mueller, J.M., Heine, A., Klebe, G. Description: Crystal Structure of the Protein-Kinase A catalyt...

New page

'''Unreleased structure'''

The entry 6i2c is ON HOLD until Paper Publication

Authors: Mueller, J.M., Heine, A., Klebe, G.

Description: Crystal Structure of the Protein-Kinase A catalytic subunit from Cricetulus Griseus in complex with compounds RKp182 and Fasudil
[[Category: Unreleased Structures]]
[[Category: Heine, A]]
[[Category: Mueller, J.M]]
[[Category: Klebe, G]]