6i2i - Revision history

OCA at 01:37, 11 April 2020

OCA — Sat, 11 Apr 2020 01:37:28 GMT

←Older revision		Revision as of 01:37, 11 April 2020
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	<SX load='6i2i' size='340' side='right' viewer='molstar' caption='[[6i2i]], [[Resolution\|resolution]] 3.60Å' scene=''>		<SX load='6i2i' size='340' side='right' viewer='molstar' caption='[[6i2i]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2I OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I2I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2i OCA], [http://pdbe.org/6i2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2i RCSB], [http://www.ebi.ac.uk/pdbsum/6i2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2i OCA], [http://pdbe.org/6i2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2i RCSB], [http://www.ebi.ac.uk/pdbsum/6i2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 6i2i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6i2i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:59, 6 March 2020

OCA — Fri, 06 Mar 2020 21:59:52 GMT

←Older revision		Revision as of 21:59, 6 March 2020
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	==Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)==		==Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)==
-	<~~StructureSection~~ load='6i2i' size='340' side='right'caption='[[6i2i]], [[Resolution\|resolution]] 3.60Å' scene=''>	+	<SX load='6i2i' size='340' side='right' viewer='molstar' caption='[[6i2i]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6i2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2I FirstGlance]. <br>		<table><tr><td colspan='2'>[[6i2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2I FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 15:25, 28 August 2019

OCA — Wed, 28 Aug 2019 15:25:56 GMT

←Older revision		Revision as of 15:25, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6i2i is ~~ON HOLD~~	+	==Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)==
		+	<StructureSection load='6i2i' size='340' side='right'caption='[[6i2i]], [[Resolution\|resolution]] 3.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2I FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2i OCA], [http://pdbe.org/6i2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2i RCSB], [http://www.ebi.ac.uk/pdbsum/6i2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_HUMAN TBA1B_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB5_HUMAN TBB5_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	EML4 is a microtubule-associated protein that promotes microtubule stability. We investigated its regulation across the cell cycle and found that EML4 was distributed as punctate foci along the microtubule lattice in interphase but exhibited reduced association with spindle microtubules in mitosis. Microtubule sedimentation and cryo-electron microscopy with 3D reconstruction revealed that the basic N-terminal domain of EML4 mediated its binding to the acidic C-terminal tails of alpha- and beta-tubulin on the microtubule surface. The mitotic kinases NEK6 and NEK7 phosphorylated the EML4 N-terminal domain at Ser(144) and Ser(146) in vitro, and depletion of these kinases in cells led to increased EML4 binding to microtubules in mitosis. An S144A-S146A double mutant not only bound inappropriately to mitotic microtubules but also increased their stability and interfered with chromosome congression. In addition, constitutive activation of NEK6 or NEK7 reduced the association of EML4 with interphase microtubules. Together, these data support a model in which NEK6- and NEK7-dependent phosphorylation promotes the dissociation of EML4 from microtubules in mitosis in a manner that is required for efficient chromosome congression.

-	~~Authors~~:	+	Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.,Adib R, Montgomery JM, Atherton J, O'Regan L, Richards MW, Straatman KR, Roth D, Straube A, Bayliss R, Moores CA, Fry AM Sci Signal. 2019 Aug 13;12(594). pii: 12/594/eaaw2939. doi:, 10.1126/scisignal.aaw2939. PMID:31409757<ref>PMID:31409757</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6i2i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Atherton, J M]]
		+	[[Category: Moores, C A]]
		+	[[Category: Eml]]
		+	[[Category: Hela]]
		+	[[Category: Microtubule]]
		+	[[Category: Structural protein]]
		+	[[Category: Tubulin]]

OCA: Protected "6i2i" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:39:47 GMT

Protected "6i2i" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:39, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6i2i is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Nov 2018 10:39:45 GMT

New page: '''Unreleased structure''' The entry 6i2i is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6i2i is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]