6i5v - Revision history

OCA at 12:34, 17 July 2019

OCA — Wed, 17 Jul 2019 12:34:09 GMT

←Older revision		Revision as of 12:34, 17 July 2019
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	<StructureSection load='6i5v' size='340' side='right'caption='[[6i5v]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='6i5v' size='340' side='right'caption='[[6i5v]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifan Bifan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Blac_00780 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=302911 BIFAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i5v OCA], [http://pdbe.org/6i5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i5v RCSB], [http://www.ebi.ac.uk/pdbsum/6i5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i5v OCA], [http://pdbe.org/6i5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i5v RCSB], [http://www.ebi.ac.uk/pdbsum/6i5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i5v ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bifan]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ejby, M]]		[[Category: Ejby, M]]

OCA at 05:58, 17 April 2019

OCA — Wed, 17 Apr 2019 05:58:18 GMT

←Older revision		Revision as of 05:58, 17 April 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6i5v is ~~ON HOLD~~	+	==BlMnBP1 binding protein of an ABC transporter from Bifidobacterium animalis subsp. lactis ATCC27673 in complex with mannotriose==
		+	<StructureSection load='6i5v' size='340' side='right'caption='[[6i5v]], [[Resolution\|resolution]] 2.01Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i5v OCA], [http://pdbe.org/6i5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i5v RCSB], [http://www.ebi.ac.uk/pdbsum/6i5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i5v ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human gut bifidobacteria rely on ATP-binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide-specific solute binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize BlMnBP1 and BlMnBP2, the two SBPs associated to the beta-manno-oligosaccharide (MnOS) ABC transporter in Bifidobacterium animalis subsp. lactis. Despite similar overall specificity and preference to mannotriose (Kd approximately 80 nM), affinity of BlMnBP1 is up to 2570-fold higher for disaccharides than BlMnBP2. Structural analysis revealed a substitution of an asparagine that recognizes the mannosyl at position 2 in BlMnBP1, by a glycine in BlMnBP2, which affects substrate affinity. Both substitution-types occur in bifidobacterial SBPs, but BlMnBP1-like variants prevail in human-gut isolates. B. animalis subsp. lactis ATCC27673 showed growth on gluco- and galactomannans and was able to outcompete a mannan-degrading Bacteroides ovatus strain in co-cultures, attesting the efficiency of this ABC uptake system. By contrast, a strain that lacks this transporter failed to grow on mannan. This study highlights SBP diversification as a possible strategy to modulate oligosaccharide uptake preferences of bifidobacterial ABC-transporters during adaptation to specific ecological niches. Efficient metabolism of galactomannan by distinct bifidobacteria, merits evaluating this plant glycan as a potential prebiotic. This article is protected by copyright. All rights reserved.

-	~~Authors: Abou Hachem, M~~., Ejby, M., Guskov, A., Slotboom, D.J.	+	Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on beta-mannan possess distinct manno-oligosaccharide binding profiles.,Ejby M, Guskov A, Pichler MJ, Zanten GC, Schoof E, Saburi W, Slotboom DJ, Abou Hachem M Mol Microbiol. 2019 Apr 4. doi: 10.1111/mmi.14257. PMID:30947380<ref>PMID:30947380</ref>

-	~~Description: BlMnBP1 binding protein~~ of ~~an ABC transporter from Bifidobacterium animalis subsp~~. ~~lactis ATCC27673 in complex with mannotriose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Abou Hachem, M]]~~	+	<div class="pdbe-citations 6i5v" style="background-color:#fffaf0;"></div>
-	~~[[Category: Guskov, A]]~~	+	== References ==
-	[[Category: ~~Slotboom, D.J~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ejby, M]]		[[Category: Ejby, M]]
		+	[[Category: Guskov, A]]
		+	[[Category: Hachem, M Abou]]
		+	[[Category: Slotboom, D J]]
		+	[[Category: Bifidobacterium animalis subsp. lactis atcc27673]]
		+	[[Category: Blac_00780]]
		+	[[Category: Mannobiose]]
		+	[[Category: Protein transport]]
		+	[[Category: Solute binding protein]]

OCA: Protected "6i5v" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 18:37:10 GMT

Protected "6i5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:37, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6i5v is ON HOLD Authors: Abou Hachem, M., Ejby, M., Guskov, A., Slotboom, D.J. Description: BlMnBP1 binding protein of an ABC transporter from Bifi...

OCA — Sun, 02 Dec 2018 18:37:08 GMT

New page: '''Unreleased structure''' The entry 6i5v is ON HOLD Authors: Abou Hachem, M., Ejby, M., Guskov, A., Slotboom, D.J. Description: BlMnBP1 binding protein of an ABC transporter from Bifi...

New page

'''Unreleased structure'''

The entry 6i5v is ON HOLD

Authors: Abou Hachem, M., Ejby, M., Guskov, A., Slotboom, D.J.

Description: BlMnBP1 binding protein of an ABC transporter from Bifidobacterium animalis subsp. lactis ATCC27673 in complex with mannotriose
[[Category: Unreleased Structures]]
[[Category: Abou Hachem, M]]
[[Category: Guskov, A]]
[[Category: Slotboom, D.J]]
[[Category: Ejby, M]]