6i8g - Revision history

OCA at 11:49, 24 January 2024

OCA — Wed, 24 Jan 2024 11:49:14 GMT

←Older revision		Revision as of 11:49, 24 January 2024
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	<StructureSection load='6i8g' size='340' side='right'caption='[[6i8g]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='6i8g' size='340' side='right'caption='[[6i8g]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i8g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~] and [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I8G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I8G FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~EDS1 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3702 ARATH])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.344Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8g OCA], [~~http~~://pdbe.org/6i8g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6i8g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6i8g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8g OCA], [https://pdbe.org/6i8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i8g RCSB], [https://www.ebi.ac.uk/pdbsum/6i8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH]] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>	+	[https://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6i8g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6i8g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Niefind, K]]	+	[[Category: Niefind K]]
-	[[Category: Toelzer, C]]	+	[[Category: Toelzer C]]
-	[[Category: Voss, M]]	+	[[Category: Voss M]]
-	~~[[Category: Alpha/beta hydrolase fold]]~~	+
-	~~[[Category: Enhanced disease susceptibility 1]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Nanobody]]~~	+
-	~~[[Category: Plant innate immune system~~]]	+

OCA at 11:32, 13 November 2019

OCA — Wed, 13 Nov 2019 11:32:46 GMT

←Older revision		Revision as of 11:32, 13 November 2019
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	<StructureSection load='6i8g' size='340' side='right'caption='[[6i8g]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='6i8g' size='340' side='right'caption='[[6i8g]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i8g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I8G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i8g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I8G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8g OCA], [http://pdbe.org/6i8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i8g RCSB], [http://www.ebi.ac.uk/pdbsum/6i8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8g ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8g OCA], [http://pdbe.org/6i8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i8g RCSB], [http://www.ebi.ac.uk/pdbsum/6i8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
		+	[[Category: Camelus glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]

OCA at 10:20, 2 October 2019

OCA — Wed, 02 Oct 2019 10:20:03 GMT

←Older revision		Revision as of 10:20, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6i8g is ~~ON HOLD~~	+	==Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB73==
		+	<StructureSection load='6i8g' size='340' side='right'caption='[[6i8g]], [[Resolution\|resolution]] 2.34Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i8g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I8G FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8g OCA], [http://pdbe.org/6i8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i8g RCSB], [http://www.ebi.ac.uk/pdbsum/6i8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH]] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In plant innate immunity, enhanced disease susceptibility 1 (EDS1) integrates all pathogen-induced signals transmitted by TIR-type NLR receptors. Driven by an N-terminal alpha/beta-hydrolase-folded domain with a protruding interaction helix, EDS1 assembles with two homologs, phytoalexin-deficient 4 (PAD4) and senescence-associated gene 101 (SAG101). The resulting heterodimers are critical for EDS1 function and structurally well characterized. Here, we resolve solution and crystal structures of unbound Arabidopsis thaliana EDS1 (AtEDS1) using nanobodies for crystallization. These structures, together with gel filtration and immunoprecipitation data, show that PAD4/SAG101-unbound AtEDS1 is stable as a monomer and does not form the homodimers recorded in public databases. Its PAD4/SAG101 anchoring helix is disordered unless engaged in protein/protein interactions. As in the complex with SAG101, monomeric AtEDS1 has a substrate-inaccessible esterase triad with a blocked oxyanion hole and without space for a covalent acyl intermediate. These new structures suggest that the AtEDS1 monomer represents an inactive or pre-activated ground state.

-	~~Authors: Niefind, K~~., Voss, M., Toelzer, C.	+	Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.,Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K J Struct Biol. 2019 Sep 21. pii: S1047-8477(19)30201-1. doi:, 10.1016/j.jsb.2019.09.007. PMID:31550533<ref>PMID:31550533</ref>

-	~~Description: Structure~~ of the ~~plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB73~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Voss, M]]~~	+	<div class="pdbe-citations 6i8g" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Toelzer, C~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]
		+	[[Category: Toelzer, C]]
		+	[[Category: Voss, M]]
		+	[[Category: Alpha/beta hydrolase fold]]
		+	[[Category: Enhanced disease susceptibility 1]]
		+	[[Category: Immune system]]
		+	[[Category: Nanobody]]
		+	[[Category: Plant innate immune system]]

OCA at 03:54, 17 April 2019

OCA — Wed, 17 Apr 2019 03:54:43 GMT

←Older revision		Revision as of 03:54, 17 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6i8g is ON HOLD ~~until Paper Publication~~	+	The entry 6i8g is ON HOLD

	Authors: Niefind, K., Voss, M., Toelzer, C.		Authors: Niefind, K., Voss, M., Toelzer, C.

-	Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ~~1AT73~~	+	Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB73
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Voss, M]]		[[Category: Voss, M]]
	[[Category: Toelzer, C]]		[[Category: Toelzer, C]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]

OCA: Protected "6i8g" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 18:44:51 GMT

Protected "6i8g" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:44, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6i8g is ON HOLD until Paper Publication Authors: Niefind, K., Voss, M., Toelzer, C. Description: Structure of the plant immune signaling node EDS1 ...

OCA — Sun, 02 Dec 2018 18:44:50 GMT

New page: '''Unreleased structure''' The entry 6i8g is ON HOLD until Paper Publication Authors: Niefind, K., Voss, M., Toelzer, C. Description: Structure of the plant immune signaling node EDS1 ...

New page

'''Unreleased structure'''

The entry 6i8g is ON HOLD until Paper Publication

Authors: Niefind, K., Voss, M., Toelzer, C.

Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody 1AT73
[[Category: Unreleased Structures]]
[[Category: Voss, M]]
[[Category: Toelzer, C]]
[[Category: Niefind, K]]