6i8v - Revision history

OCA at 11:49, 24 January 2024

OCA — Wed, 24 Jan 2024 11:49:32 GMT

←Older revision		Revision as of 11:49, 24 January 2024
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	<StructureSection load='6i8v' size='340' side='right'caption='[[6i8v]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='6i8v' size='340' side='right'caption='[[6i8v]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i8v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I8V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I8V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.991Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8v OCA], [~~http~~://pdbe.org/6i8v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6i8v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6i8v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8v ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8v OCA], [https://pdbe.org/6i8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i8v RCSB], [https://www.ebi.ac.uk/pdbsum/6i8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KTRC_BACSU KTRC_BACSU]] Catalytic subunit of the KtrCD potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.<ref>PMID:12562800</ref>	+	[https://www.uniprot.org/uniprot/KTRC_BACSU KTRC_BACSU] Catalytic subunit of the KtrCD potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.<ref>PMID:12562800</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Morais-Cabral~~, J H~~]]	+	[[Category: Morais-Cabral JH]]
-	[[Category: Rocha, R]]	+	[[Category: Rocha R]]
-	~~[[Category: Ion transport]]~~	+
-	~~[[Category: Metal transport~~]]	+

OCA at 08:59, 30 October 2019

OCA — Wed, 30 Oct 2019 08:59:09 GMT

←Older revision		Revision as of 08:59, 30 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6i8v is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==KtrC with ATP bound==
		+	<StructureSection load='6i8v' size='340' side='right'caption='[[6i8v]], [[Resolution\|resolution]] 1.99Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i8v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I8V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i8v OCA], [http://pdbe.org/6i8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i8v RCSB], [http://www.ebi.ac.uk/pdbsum/6i8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i8v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KTRC_BACSU KTRC_BACSU]] Catalytic subunit of the KtrCD potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.<ref>PMID:12562800</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RCK (regulating conductance of K(+)) domains are common regulatory domains that control the activity of eukaryotic and prokaryotic K(+) channels and transporters. In bacteria these domains play roles in osmoregulation, regulation of turgor and membrane potential and in pH homeostasis. Whole-genome sequencing unveiled RCK gene redundancy, however the biological role of this redundancy is not well understood. In Bacillus subtilis, there are two closely related RCK domain proteins (KtrA and KtrC) that regulate the activity of the Ktr cation channels. KtrA has been well characterized but little is known about KtrC. We have characterized the structural and biochemical proprieties of KtrC and conclude that KtrC binds ATP and ADP, just like KtrA. However, in solution KtrC exist in a dynamic equilibrium between octamers and non-octameric species that is dependent on the bound ligand, with ATP destabilizing the octameric ring relative to ADP. Accordingly, KtrC-ADP crystal structures reveal closed octameric rings similar to those in KtrA, while KtrC-ATP adopts an open assembly with RCK domains forming a super-helix. In addition, both KtrC-ATP and -ADP octamers are stabilized by the signaling molecule cyclic-di-AMP, which binds to KtrC with high affinity. In contrast, c-di-AMP binds with 100-fold lower affinity to KtrA. Despite these differences we show with an E. coli complementation assay that KtrC and KtrA are interchangeable and able to form functional transporters with both KtrB and KtrD. The distinctive properties of KtrC, in particular ligand-dependent assembly/disassembly, suggest that this protein has a specific physiological role that is distinct from KtrA.

-	~~Authors: Rocha~~, R., Morais-Cabral, J.H.	+	Characterization of the molecular properties of KtrC, a second RCK domain that regulates a Ktr channel in Bacillus subtilis.,Rocha R, Teixeira-Duarte CM, Jorge JMP, Morais-Cabral JH J Struct Biol. 2019 Mar 1;205(3):34-43. doi: 10.1016/j.jsb.2019.02.002. Epub 2019, Feb 10. PMID:30753894<ref>PMID:30753894</ref>

-	~~Description~~: ~~KtrC with ATP bound~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6i8v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Morais-Cabral, J H]]
	[[Category: Rocha, R]]		[[Category: Rocha, R]]
-	[[Category: ~~Morais-Cabral, J.H~~]]	+	[[Category: Ion transport]]
		+	[[Category: Metal transport]]

OCA: Protected "6i8v" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 18:45:53 GMT

Protected "6i8v" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:45, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6i8v is ON HOLD until Paper Publication Authors: Rocha, R., Morais-Cabral, J.H. Description: KtrC with ATP bound [[Category: Unreleased Structures]...

OCA — Sun, 02 Dec 2018 18:45:52 GMT

New page: '''Unreleased structure''' The entry 6i8v is ON HOLD until Paper Publication Authors: Rocha, R., Morais-Cabral, J.H. Description: KtrC with ATP bound [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 6i8v is ON HOLD until Paper Publication

Authors: Rocha, R., Morais-Cabral, J.H.

Description: KtrC with ATP bound
[[Category: Unreleased Structures]]
[[Category: Rocha, R]]
[[Category: Morais-Cabral, J.H]]