OCA at 15:26, 28 August 2019

OCA — Wed, 28 Aug 2019 15:26:18 GMT

←Older revision		Revision as of 15:26, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6i97 is ~~ON HOLD~~	+	==Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa in complex with ferrioxamine B and a C-terminal TonB fragment==
		+	<StructureSection load='6i97' size='340' side='right'caption='[[6i97]], [[Resolution\|resolution]] 3.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6i97]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I97 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i97 OCA], [http://pdbe.org/6i97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i97 RCSB], [http://www.ebi.ac.uk/pdbsum/6i97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i97 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A2R3J1C6_PSEAI A0A2R3J1C6_PSEAI]] Interacts with outer membrane receptor proteins that carry out high-affinity binding and energy dependent uptake into the periplasmic space of specific substrates. It could act to transduce energy from the cytoplasmic membrane to specific energy-requiring processes in the outer membrane, resulting in the release into the periplasm of ligands bound by these outer membrane proteins.[RuleBase:RU362123]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many microbes and fungi acquire the essential ion Fe(3+) through the synthesis and secretion of high-affinity chelators termed siderophores. In Gram-negative bacteria, these ferric-siderophore complexes are actively taken up using highly specific TonB-dependent transporters (TBDTs) located in the outer bacterial membrane (OM). However, the detailed mechanism of how the inner-membrane protein TonB connects to the transporters in the OM as well as the interplay between siderophore- and TonB-binding to the transporter is still poorly understood. Here, we present three crystal structures of the TBDT FoxA from Pseudomonas aeruginosa (containing a signalling domain) in complex with the siderophore ferrioxamine B and TonB and combine them with a detailed analysis of binding constants. The structures show that both siderophore and TonB-binding is required to form a translocation-competent state of the FoxA transporter in a two-step TonB-binding mechanism. The complex structure also indicates how TonB-binding influences the orientation of the signalling domain.

-	~~Authors: Josts~~, I., ~~Tidow~~, H.	+	Ternary structure of the outer membrane transporter FoxA with resolved signalling domain provides insights into TonB-mediated siderophore uptake.,Josts I, Veith K, Tidow H Elife. 2019 Aug 6;8. pii: 48528. doi: 10.7554/eLife.48528. PMID:31385808<ref>PMID:31385808</ref>

-	~~Description: Crystal structure~~ of ~~outer membrane protein~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6i97" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Josts, I]]		[[Category: Josts, I]]
	[[Category: Tidow, H]]		[[Category: Tidow, H]]
		+	[[Category: Membrane protein]]
		+	[[Category: Outer membrane protein iron uptake]]

OCA: Protected "6i97" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 18:46:49 GMT

Protected "6i97" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:46, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6i97 is ON HOLD Authors: Josts, I., Tidow, H. Description: Crystal structure of outer membrane protein Category: Unreleased Structures [[Catego...

OCA — Sun, 02 Dec 2018 18:46:48 GMT

New page: '''Unreleased structure''' The entry 6i97 is ON HOLD Authors: Josts, I., Tidow, H. Description: Crystal structure of outer membrane protein Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 6i97 is ON HOLD

Authors: Josts, I., Tidow, H.

Description: Crystal structure of outer membrane protein
[[Category: Unreleased Structures]]
[[Category: Josts, I]]
[[Category: Tidow, H]]

6i97 - Revision history

OCA at 15:26, 28 August 2019

OCA: Protected "6i97" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6i97 is ON HOLD Authors: Josts, I., Tidow, H. Description: Crystal structure of outer membrane protein Category: Unreleased Structures [[Catego...