6ib4 - Revision history

OCA: Redirecting to 6rvw

OCA — Wed, 12 Jun 2019 05:20:34 GMT

Redirecting to 6rvw

←Older revision		Revision as of 05:20, 12 June 2019
Line 1:		Line 1:
-		+	#REDIRECT [[6rvw]] This PDB entry is obsolete and replaced by 6rvw
-	~~==Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.==~~	+
-	~~<StructureSection load='6ib4' size='340' side='right'caption='~~[[~~6ib4]], [[Resolution\|resolution]] 3.70Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[6ib4~~]] is a 264 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IB4 FirstGlance]. <br>	+
-	~~</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Symmetrical protein cages have evolved to fulfil diverse roles in nature, including compartmentalization~~ and cargo delivery(1), and have inspired synthetic biologists to create novel protein assemblies via the precise manipulation of protein-protein interfaces. Despite the impressive array of protein cages produced in the laboratory, the design of inducible assemblies remains challenging(2,3). Here we demonstrate an ultra-stable artificial protein cage, the assembly and disassembly of which can be controlled by metal coordination at the protein-protein interfaces. The addition of a gold (I)-triphenylphosphine compound to a cysteine-substituted, 11-mer protein ring triggers supramolecular self-assembly, which generates monodisperse cage structures with masses greater than 2 MDa. The geometry of these structures is based on the Archimedean snub cube and is, to our knowledge, unprecedented. Cryo-electron microscopy confirms that the assemblies are held together by 120 S-Au(i)-S staples between the protein oligomers, and exist in two chiral forms. The cage shows extreme chemical and thermal stability, yet it readily disassembles upon exposure to reducing agents. As well as gold, mercury(II) is also found to enable formation of the protein cage. This work establishes an approach for linking protein components into robust, higher-order structures, and expands the design space available for supramolecular assemblies to include previously unexplored geometries.	+
-		+
-	An ultra-stable gold-coordinated protein cage displaying reversible assembly.,Malay AD, Miyazaki N, Biela A, Chakraborti S, Majsterkiewicz K, Stupka I, Kaplan CS, Kowalczyk A, Piette BMAG, Hochberg GKA, Wu D, Wrobel TP, Fineberg A, Kushwah MS, Kelemen M, Vavpetic P, Pelicon P, Kukura P, Benesch JLP, Iwasaki K, Heddle JG Nature. 2019 May;569(7756):438-442. doi: 10.1038/s41586-019-1185-4. Epub 2019 May, 8. PMID:31068697<ref>PMID:31068697</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6ib4" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Atcc 12980]]~~	+
-	~~[[Category: Large Structures]]~~	+
-	~~[[Category: Biela, A P]]~~	+
-	~~[[Category: Heddle, J G]]~~	+
-	~~[[Category: Iwasaki, K]]~~	+
-	~~[[Category: Malay, A D]]~~	+
-	~~[[Category: Miyazaki, N]]~~	+
-	~~[[Category: Gold binding]]~~	+
-	~~[[Category: Protein cage]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Snub cube]]~~	+
-	~~[[Category: Trap]]~~	+

OCA at 07:33, 23 May 2019

OCA — Thu, 23 May 2019 07:33:06 GMT

←Older revision		Revision as of 07:33, 23 May 2019
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Symmetrical protein cages have evolved to fulfil diverse roles in nature, including compartmentalization and cargo delivery(1), and have inspired synthetic biologists to create novel protein assemblies via the precise manipulation of protein-protein interfaces. Despite the impressive array of protein cages produced in the laboratory, the design of inducible assemblies remains challenging(2,3). Here we demonstrate an ultra-stable artificial protein cage, the assembly and disassembly of which can be controlled by metal coordination at the protein-protein interfaces. The addition of a gold (I)-triphenylphosphine compound to a cysteine-substituted, 11-mer protein ring triggers supramolecular self-assembly, which generates monodisperse cage structures with masses greater than 2 MDa. The geometry of these structures is based on the Archimedean snub cube and is, to our knowledge, unprecedented. Cryo-electron microscopy confirms that the assemblies are held together by 120 S-Au(i)-S staples between the protein oligomers, and exist in two chiral forms. The cage shows extreme chemical and thermal stability, yet it readily disassembles upon exposure to reducing agents. As well as gold, mercury(II) is also found to enable formation of the protein cage. This work establishes an approach for linking protein components into robust, higher-order structures, and expands the design space available for supramolecular assemblies to include previously unexplored geometries.
		+
		+	An ultra-stable gold-coordinated protein cage displaying reversible assembly.,Malay AD, Miyazaki N, Biela A, Chakraborti S, Majsterkiewicz K, Stupka I, Kaplan CS, Kowalczyk A, Piette BMAG, Hochberg GKA, Wu D, Wrobel TP, Fineberg A, Kushwah MS, Kelemen M, Vavpetic P, Pelicon P, Kukura P, Benesch JLP, Iwasaki K, Heddle JG Nature. 2019 May;569(7756):438-442. doi: 10.1038/s41586-019-1185-4. Epub 2019 May, 8. PMID:31068697<ref>PMID:31068697</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ib4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:15, 21 May 2019

OCA — Tue, 21 May 2019 08:15:23 GMT

←Older revision		Revision as of 08:15, 21 May 2019
Line 1:		Line 1:

	==Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.==		==Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.==
-	<StructureSection load='6ib4' size='340' side='right' caption='[[6ib4]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='6ib4' size='340' side='right'caption='[[6ib4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ib4]] is a 264 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IB4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ib4]] is a 264 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IB4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 12980]]
		+	[[Category: Large Structures]]
	[[Category: Biela, A P]]		[[Category: Biela, A P]]
	[[Category: Heddle, J G]]		[[Category: Heddle, J G]]

OCA at 07:30, 6 March 2019

OCA — Wed, 06 Mar 2019 07:30:35 GMT

←Older revision		Revision as of 07:30, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6ib4 is ON HOLD~~	+	==Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.==
-		+	<StructureSection load='6ib4' size='340' side='right' caption='[[6ib4]], [[Resolution\|resolution]] 3.70Å' scene=''>
-	~~Authors: Malay, A.D., Miyazaki, N., Biela, A.P., Iwasaki, K., Heddle, J.G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ib4]] is a 264 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IB4 FirstGlance]. <br>
-	~~Description:~~ Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ib4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ib4 OCA], [http://pdbe.org/6ib4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ib4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ib4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ib4 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Biela, A P]]
		+	[[Category: Heddle, J G]]
		+	[[Category: Iwasaki, K]]
		+	[[Category: Malay, A D]]
	[[Category: Miyazaki, N]]		[[Category: Miyazaki, N]]
-	[[Category: ~~Biela, A.P~~]]	+	[[Category: Gold binding]]
-	[[Category: ~~Heddle, J.G~~]]	+	[[Category: Protein cage]]
-	[[Category: ~~Iwasaki, K~~]]	+	[[Category: Rna binding protein]]
-	[[Category: ~~Malay, A.D~~]]	+	[[Category: Snub cube]]
		+	[[Category: Trap]]

OCA: Protected "6ib4" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2018 05:04:23 GMT

Protected "6ib4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:04, 5 December 2018

OCA: New page: '''Unreleased structure''' The entry 6ib4 is ON HOLD Authors: Malay, A.D., Miyazaki, N., Biela, A.P., Iwasaki, K., Heddle, J.G. Description: Structure of right-handed protein cage cons...

OCA — Wed, 05 Dec 2018 05:04:22 GMT

New page: '''Unreleased structure''' The entry 6ib4 is ON HOLD Authors: Malay, A.D., Miyazaki, N., Biela, A.P., Iwasaki, K., Heddle, J.G. Description: Structure of right-handed protein cage cons...

New page

'''Unreleased structure'''

The entry 6ib4 is ON HOLD

Authors: Malay, A.D., Miyazaki, N., Biela, A.P., Iwasaki, K., Heddle, J.G.

Description: Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by gold (I) bridges.
[[Category: Unreleased Structures]]
[[Category: Miyazaki, N]]
[[Category: Biela, A.P]]
[[Category: Heddle, J.G]]
[[Category: Iwasaki, K]]
[[Category: Malay, A.D]]