6ibu - Revision history

OCA at 11:51, 24 January 2024

OCA — Wed, 24 Jan 2024 11:51:17 GMT

←Older revision		Revision as of 11:51, 24 January 2024
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	==Apo Crh5 transglycosylase==		==Apo Crh5 transglycosylase==
-	<StructureSection load='6ibu' size='340' side='right' caption='[[6ibu]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='6ibu' size='340' side='right'caption='[[6ibu]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ibu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IBU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ibu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IBU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand~~=~~GOL:GLYCEROL'>GOL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5ndl\|5ndl]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ibu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibu OCA], [~~http~~://pdbe.org/6ibu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ibu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ibu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ibu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibu OCA], [https://pdbe.org/6ibu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ibu RCSB], [https://www.ebi.ac.uk/pdbsum/6ibu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CRF1_ASPFU CRF1_ASPFU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fungal cell wall synthesis is achieved by a balance of glycosyltransferase, hydrolase and transglycosylase activities. Transglycosylases strengthen the cell wall by forming a rigid network of crosslinks through mechanisms that remain to be explored. Here we study the function of the Aspergillus fumigatus family of five Crh transglycosylases. Although crh genes are dispensable for cell viability, simultaneous deletion of all genes renders cells sensitive to cell wall interfering compounds. In vitro biochemical assays and localisation studies demonstrate that this family of enzymes functions redundantly as transglycosylases for both chitin-glucan and chitin-chitin cell wall crosslinks. To understand the molecular basis of this acceptor promiscuity, we solved the crystal structure of A. fumigatus Crh5 (AfCrh5) in complex with a chitooligosaccharide at the resolution of 2.8 A, revealing an extensive elongated binding cleft for the donor (-4 to -1) substrate and a short acceptor (+1 to +2) binding site. Together with mutagenesis, the structure suggests a "hydrolysis product assisted" molecular mechanism favouring transglycosylation over hydrolysis.
		+
		+	Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.,Fang W, Sanz AB, Bartual SG, Wang B, Ferenbach AT, Farkas V, Hurtado-Guerrero R, Arroyo J, van Aalten DMF Nat Commun. 2019 Apr 10;10(1):1669. doi: 10.1038/s41467-019-09674-0. PMID:30971696<ref>PMID:30971696</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ibu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aalten, D M.F van~~]]	+	[[Category: Aspergillus fumigatus Af293]]
-	[[Category: ~~Bartual, S G~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fang, W~~]]	+	[[Category: Bartual SG]]
-	[[Category: ~~Cell wall assembly~~]]	+	[[Category: Fang W]]
-	[[Category: ~~Cross-link]]~~	+	[[Category: Van Aalten DMF]]
-	~~[[Category: Gh16]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Transglycosylase~~]]	+

OCA at 15:25, 27 February 2019

OCA — Wed, 27 Feb 2019 15:25:19 GMT

←Older revision		Revision as of 15:25, 27 February 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ibu ~~is ON HOLD until Paper Publication~~	+	==Apo Crh5 transglycosylase==
-		+	<StructureSection load='6ibu' size='340' side='right' caption='[[6ibu]], [[Resolution\|resolution]] 2.25Å' scene=''>
-	~~Authors~~: ~~Bartual, S~~.G.~~, Fang, W~~.~~, van Aalten, D~~.M.F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ibu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IBU FirstGlance]. <br>
-	~~Description~~: ~~Apo Crh5 transglycosylase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ndl\|5ndl]]</td></tr>
-	[[Category: ~~Van~~ Aalten, D.M.F]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ibu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibu OCA], [http://pdbe.org/6ibu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ibu RCSB], [http://www.ebi.ac.uk/pdbsum/6ibu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibu ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aalten, D M.F van]]
		+	[[Category: Bartual, S G]]
	[[Category: Fang, W]]		[[Category: Fang, W]]
-	[[Category: ~~Bartual, S.G~~]]	+	[[Category: Cell wall assembly]]
		+	[[Category: Cross-link]]
		+	[[Category: Gh16]]
		+	[[Category: Hydrolase]]
		+	[[Category: Transglycosylase]]

OCA: Protected "6ibu" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:06:20 GMT

Protected "6ibu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6ibu is ON HOLD until Paper Publication Authors: Bartual, S.G., Fang, W., van Aalten, D.M.F. Description: Apo Crh5 transglycosylase [[Category: Unr...

OCA — Wed, 12 Dec 2018 05:06:19 GMT

New page: '''Unreleased structure''' The entry 6ibu is ON HOLD until Paper Publication Authors: Bartual, S.G., Fang, W., van Aalten, D.M.F. Description: Apo Crh5 transglycosylase [[Category: Unr...

New page

'''Unreleased structure'''

The entry 6ibu is ON HOLD until Paper Publication

Authors: Bartual, S.G., Fang, W., van Aalten, D.M.F.

Description: Apo Crh5 transglycosylase
[[Category: Unreleased Structures]]
[[Category: Van Aalten, D.M.F]]
[[Category: Fang, W]]
[[Category: Bartual, S.G]]