6ici - Revision history

OCA at 09:37, 22 November 2023

OCA — Wed, 22 Nov 2023 09:37:01 GMT

←Older revision		Revision as of 09:37, 22 November 2023
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	<StructureSection load='6ici' size='340' side='right'caption='[[6ici]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6ici' size='340' side='right'caption='[[6ici]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ici]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ICI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ICI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ici]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ICI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ICI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/F-actin_monooxygenase F-actin monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.225 1.14.13.225]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ici FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ici OCA], [~~http~~://pdbe.org/6ici PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ici RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ici PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ici ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ici FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ici OCA], [https://pdbe.org/6ici PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ici RCSB], [https://www.ebi.ac.uk/pdbsum/6ici PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ici ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MICA3_HUMAN MICA3_HUMAN]] Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity). Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process.<ref>PMID:21596566</ref>	+	[https://www.uniprot.org/uniprot/MICA3_HUMAN MICA3_HUMAN] Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity). Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process.<ref>PMID:21596566</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~F-actin monooxygenase~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hwang~~, K Y~~]]	+	[[Category: Hwang KY]]
-	[[Category: Kim~~, J S]]~~	+	[[Category: Kim JS]]
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:37, 4 March 2020

OCA — Wed, 04 Mar 2020 06:37:42 GMT

←Older revision		Revision as of 06:37, 4 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ici is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of human MICAL3==
		+	<StructureSection load='6ici' size='340' side='right'caption='[[6ici]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ici]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ICI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ICI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/F-actin_monooxygenase F-actin monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.225 1.14.13.225] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ici FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ici OCA], [http://pdbe.org/6ici PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ici RCSB], [http://www.ebi.ac.uk/pdbsum/6ici PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ici ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MICA3_HUMAN MICA3_HUMAN]] Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity). Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process.<ref>PMID:21596566</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MICAL is an oxidoreductase that participates in cytoskeleton reorganization via actin disassembly in the presence of NADPH. Although three MICALs (MICAL1, MICAL2 and MICAL3) have been identified in mammals, only the structure of mouse MICAL1 has been reported. Here, the first crystal structure of human MICAL3, which contains the flavin-containing monooxygenase (FMO) and calponin-homology (CH) domains, is reported. MICAL3 has an FAD/NADP-binding Rossmann-fold domain for mono-oxygenase activity like MICAL1. The FMO and CH domains of both MICAL3 and MICAL1 are highly similar in structure, but superimposition of the two structures shows a different relative position of the CH domain in the asymmetric unit. Based on kinetic analyses, the catalytic efficiency of MICAL3 dramatically increased on adding F-actin only when the CH domain was available. However, this did not occur when two residues, Glu213 and Arg530, were mutated in the FMO and CH domains, respectively. Overall, MICAL3 is structurally highly similar to MICAL1, which suggests that they may adopt the same catalytic mechanism, but the difference in the relative position of the CH domain produces a difference in F-actin substrate specificity.

-	~~Authors: Hwang, K.Y~~., Kim, J.S.	+	Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3.,Kim J, Lee H, Roh YJ, Kim HU, Shin D, Kim S, Son J, Lee A, Kim M, Park J, Hwang SY, Kim K, Lee YK, Jung HS, Hwang KY, Lee BC IUCrJ. 2020 Jan 1;7(Pt 1):90-99. doi: 10.1107/S2052252519015409. eCollection 2020, Jan 1. PMID:31949908<ref>PMID:31949908</ref>

-	~~Description: Crystal structure~~ of ~~human MICAL3~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Hwang, K.Y]]	+	<div class="pdbe-citations 6ici" style="background-color:#fffaf0;"></div>
-	[[Category: Kim, J.S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: F-actin monooxygenase]]
		+	[[Category: Large Structures]]
		+	[[Category: Hwang, K Y]]
		+	[[Category: Kim, J S]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Oxidoreductase]]

OCA at 07:54, 26 February 2020

OCA — Wed, 26 Feb 2020 07:54:48 GMT

←Older revision		Revision as of 07:54, 26 February 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ici is ON HOLD	+	The entry 6ici is ON HOLD until Paper Publication

	Authors: Hwang, K.Y., Kim, J.S.		Authors: Hwang, K.Y., Kim, J.S.

OCA at 12:56, 29 January 2020

OCA — Wed, 29 Jan 2020 12:56:14 GMT

←Older revision		Revision as of 12:56, 29 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ici is ON HOLD ~~until Mar 06 2021~~	+	The entry 6ici is ON HOLD

	Authors: Hwang, K.Y., Kim, J.S.		Authors: Hwang, K.Y., Kim, J.S.

OCA at 14:52, 14 August 2019

OCA — Wed, 14 Aug 2019 14:52:31 GMT

←Older revision		Revision as of 14:52, 14 August 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ici is ON HOLD until ~~Paper Publication~~	+	The entry 6ici is ON HOLD until Mar 06 2021

	Authors: Hwang, K.Y., Kim, J.S.		Authors: Hwang, K.Y., Kim, J.S.

OCA at 06:19, 26 September 2018

OCA — Wed, 26 Sep 2018 06:19:04 GMT

←Older revision		Revision as of 06:19, 26 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ici is ON HOLD	+	The entry 6ici is ON HOLD until Paper Publication

	Authors: Hwang, K.Y., Kim, J.S.		Authors: Hwang, K.Y., Kim, J.S.

OCA at 18:16, 19 September 2018

OCA — Wed, 19 Sep 2018 18:16:44 GMT

←Older revision		Revision as of 18:16, 19 September 2018
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	Authors: Hwang, K.Y., Kim, J.S.		Authors: Hwang, K.Y., Kim, J.S.

-	Description: ~~crystal~~ structure of human MICAL3	+	Description: Crystal structure of human MICAL3
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hwang, K.Y]]		[[Category: Hwang, K.Y]]
	[[Category: Kim, J.S]]		[[Category: Kim, J.S]]

OCA: Protected "6ici" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2018 06:08:55 GMT

Protected "6ici" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:08, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6ici is ON HOLD Authors: Hwang, K.Y., Kim, J.S. Description: crystal structure of human MICAL3 Category: Unreleased Structures [[Category: Hwan...

OCA — Wed, 12 Sep 2018 06:08:53 GMT

New page: '''Unreleased structure''' The entry 6ici is ON HOLD Authors: Hwang, K.Y., Kim, J.S. Description: crystal structure of human MICAL3 Category: Unreleased Structures [[Category: Hwan...

New page

'''Unreleased structure'''

The entry 6ici is ON HOLD

Authors: Hwang, K.Y., Kim, J.S.

Description: crystal structure of human MICAL3
[[Category: Unreleased Structures]]
[[Category: Hwang, K.Y]]
[[Category: Kim, J.S]]