6ien - Revision history

OCA at 10:26, 15 November 2023

OCA — Wed, 15 Nov 2023 10:26:10 GMT

←Older revision		Revision as of 10:26, 15 November 2023
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	<StructureSection load='6ien' size='340' side='right'caption='[[6ien]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='6ien' size='340' side='right'caption='[[6ien]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ien]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IEN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ien]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IEN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argH, Rv1659, MTCY06H11.24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ien OCA], [https://pdbe.org/6ien PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ien RCSB], [https://www.ebi.ac.uk/pdbsum/6ien PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ien ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ien OCA], [~~http~~://pdbe.org/6ien PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ien RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ien PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ien ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ARLY_MYCTU ARLY_MYCTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Argininosuccinate lyase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Mishra, A]]	+	[[Category: Mishra A]]
-	[[Category: Paul, A]]	+	[[Category: Paul A]]
-	[[Category: Surolia, A]]	+	[[Category: Surolia A]]
-	[[Category: Vijayan, M]]	+	[[Category: Vijayan M]]
-	~~[[Category: Arginine biosynthesis]]~~	+
-	~~[[Category: Aspartase/fumarase]]~~	+
-	~~[[Category: Concerted movement]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Tetramer~~]]	+

OCA at 06:55, 17 April 2019

OCA — Wed, 17 Apr 2019 06:55:48 GMT

←Older revision		Revision as of 06:55, 17 April 2019
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	==Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis==		==Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis==
-	<StructureSection load='6ien' size='340' side='right' caption='[[6ien]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='6ien' size='340' side='right'caption='[[6ien]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ien]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IEN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ien]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IEN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argH, Rv1659, MTCY06H11.24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ien OCA], [http://pdbe.org/6ien PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ien RCSB], [http://www.ebi.ac.uk/pdbsum/6ien PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ien ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ien OCA], [http://pdbe.org/6ien PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ien RCSB], [http://www.ebi.ac.uk/pdbsum/6ien PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ien ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Mishra, A]]		[[Category: Mishra, A]]
	[[Category: Paul, A]]		[[Category: Paul, A]]

OCA at 06:47, 21 February 2019

OCA — Thu, 21 Feb 2019 06:47:12 GMT

←Older revision		Revision as of 06:47, 21 February 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ien is ~~ON HOLD until Paper~~ Publication	+	==Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis==
		+	<StructureSection load='6ien' size='340' side='right' caption='[[6ien]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ien]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IEN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ien OCA], [http://pdbe.org/6ien PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ien RCSB], [http://www.ebi.ac.uk/pdbsum/6ien PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ien ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Argininosuccinate lyase catalyses the reversible breakdown of argininosuccinate into arginine and fumarate and is known to form tetramers in its quaternary association. The absence of structures involving competent enzymes bound to substrate/products came in the way of the precise elucidation of the catalytic mechanism of this family of proteins. Crystal structures of the enzyme from Mycobacterium tuberculosis in an unliganded form and its complex with the substrate/products have now been determined at 2.2 and 2.7 A, respectively. The refinement of the structure of the complex was bedevilled by the presence of a lattice translocation defect. The two tetramers in the apo-crystals and the one in the crystals of the liganded protein, have the same structure except for the movements associated with enzyme action. Each molecule consists of an N-domain, an M-domain, and a C-domain. The molecule consists of four binding sites, each made up of peptide stretches from three subunits. Three binding sites appear to be occupied by the ligand in the transition state, while the products occupy the fourth site. The structure exhibits the movement of a loop in the M-domain and parts of the C-domain. This is the first instance when the appropriate movements are observed in a complex with bound substrate/product. The detailed picture of the binding site, active site residues and the movements associated with catalysis thus obtained, enabled a revisit of the mechanism of action of the enzyme. (c) 2019 IUBMB Life, 9999(9999):1-10, 2019.

-	~~Authors~~:	+	Structural studies on M. tuberculosis argininosuccinate lyase and its liganded complex: Insights into catalytic mechanism.,Paul A, Mishra A, Surolia A, Vijayan M IUBMB Life. 2019 Jan 7. doi: 10.1002/iub.2000. PMID:30615268<ref>PMID:30615268</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ien" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Argininosuccinate lyase]]
		+	[[Category: Mishra, A]]
		+	[[Category: Paul, A]]
		+	[[Category: Surolia, A]]
		+	[[Category: Vijayan, M]]
		+	[[Category: Arginine biosynthesis]]
		+	[[Category: Aspartase/fumarase]]
		+	[[Category: Concerted movement]]
		+	[[Category: Lyase]]
		+	[[Category: Tetramer]]

OCA at 06:22, 17 October 2018

OCA — Wed, 17 Oct 2018 06:22:00 GMT

←Older revision		Revision as of 06:22, 17 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ien is ON HOLD	+	The entry 6ien is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ien" [edit=sysop:move=sysop]

OCA — Wed, 26 Sep 2018 06:20:19 GMT

Protected "6ien" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:20, 26 September 2018

OCA: New page: '''Unreleased structure''' The entry 6ien is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Sep 2018 06:20:17 GMT

New page: '''Unreleased structure''' The entry 6ien is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ien is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]