6im7 - Revision history

OCA at 09:45, 22 November 2023

OCA — Wed, 22 Nov 2023 09:45:57 GMT

←Older revision		Revision as of 09:45, 22 November 2023
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	<StructureSection load='6im7' size='340' side='right'caption='[[6im7]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='6im7' size='340' side='right'caption='[[6im7]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6im7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IM7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IM7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6im7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IM7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CA:CALCIUM+ION'>CA</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~cueO, yacK, b0123, JW0119 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6im7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6im7 OCA], [~~http~~://pdbe.org/6im7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6im7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6im7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6im7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6im7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6im7 OCA], [https://pdbe.org/6im7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6im7 RCSB], [https://www.ebi.ac.uk/pdbsum/6im7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6im7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CUEO_ECOLI CUEO_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ghadessy, F~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Robinson, R~~]]	+	[[Category: Ghadessy F]]
-	[[Category: ~~Wongsantichon, J~~]]	+	[[Category: Robinson R]]
-	[[Category: ~~Laccase]]~~	+	[[Category: Wongsantichon J]]
-	~~[[Category: Multicopper oxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:16, 7 August 2019

OCA — Wed, 07 Aug 2019 06:16:02 GMT

←Older revision		Revision as of 06:16, 7 August 2019
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	<StructureSection load='6im7' size='340' side='right'caption='[[6im7]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='6im7' size='340' side='right'caption='[[6im7]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6im7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IM7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IM7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6im7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IM7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IM7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cueO, yacK, b0123, JW0119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6im7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6im7 OCA], [http://pdbe.org/6im7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6im7 RCSB], [http://www.ebi.ac.uk/pdbsum/6im7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6im7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6im7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6im7 OCA], [http://pdbe.org/6im7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6im7 RCSB], [http://www.ebi.ac.uk/pdbsum/6im7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6im7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein-protein interactions (PPIs) are ubiquitous in almost all biological processes and are often corrupted in diseased states. A detailed understanding of PPIs is therefore key to understanding cellular physiology and can yield attractive therapeutic targets. Here, we describe the development and structural characterization of novel Escherichia coli CueO multi-copper oxidase variants engineered to recapitulate protein-protein interactions with commensurate modulation of their enzymatic activities. The fully integrated single-protein sensors were developed through modular grafting of ligand-specific peptides into a highly compliant and flexible methionine-rich loop of CueO. Sensitive detection of diverse ligand classes exemplified by antibodies, an E3 ligase, MDM2 proto-oncogene (MDM2), and protease (SplB from Staphylococcus aureus) was achieved in a simple mix and measure homogeneous format with visually observable colorimetric readouts. Therapeutic antagonism of MDM2 by small molecules and peptides in clinical development for treatment of cancer patients was assayed using the MDM2-binding CueO enzyme. Structural characterization of the free and MDM2-bound CueO variant provided functional insight into signal-transducing mechanisms of the engineered enzymes and highlighted the robustness of CueO as a stable and compliant scaffold for multiple applications.
		+
		+	Development and structural characterization of an engineered multi-copper oxidase reporter of protein-protein interactions.,Sana B, Chee SMQ, Wongsantichon J, Raghavan S, Robinson RC, Ghadessy FJ J Biol Chem. 2019 Apr 26;294(17):7002-7012. doi: 10.1074/jbc.RA118.007141. Epub, 2019 Feb 15. PMID:30770473<ref>PMID:30770473</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6im7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Blue copper oxidase CueO 3D structures\|Blue copper oxidase CueO 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ghadessy, F]]		[[Category: Ghadessy, F]]

OCA at 07:58, 20 March 2019

OCA — Wed, 20 Mar 2019 07:58:14 GMT

←Older revision		Revision as of 07:58, 20 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6im7 ~~is ON HOLD until Paper Publication~~	+	==CueO-12.1 multicopper oxidase==
-		+	<StructureSection load='6im7' size='340' side='right'caption='[[6im7]], [[Resolution\|resolution]] 1.97Å' scene=''>
-	~~Authors~~: ~~Wongsantichon, J~~.~~, Robinson, R~~.~~, Ghadessy, F~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6im7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IM7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IM7 FirstGlance]. <br>
-	~~Description~~: ~~CueO~~-12.~~1 multicopper oxidase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6im7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6im7 OCA], [http://pdbe.org/6im7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6im7 RCSB], [http://www.ebi.ac.uk/pdbsum/6im7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6im7 ProSAT]</span></td></tr>
-	[[Category: ~~Wongsantichon, J~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ghadessy, F]]		[[Category: Ghadessy, F]]
	[[Category: Robinson, R]]		[[Category: Robinson, R]]
		+	[[Category: Wongsantichon, J]]
		+	[[Category: Laccase]]
		+	[[Category: Multicopper oxidase]]
		+	[[Category: Oxidoreductase]]

OCA at 05:06, 5 December 2018

OCA — Wed, 05 Dec 2018 05:06:30 GMT

←Older revision		Revision as of 05:06, 5 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6im7 is ON HOLD	+	The entry 6im7 is ON HOLD until Paper Publication

	Authors: Wongsantichon, J., Robinson, R., Ghadessy, F.		Authors: Wongsantichon, J., Robinson, R., Ghadessy, F.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Wongsantichon, J]]		[[Category: Wongsantichon, J]]
-	[[Category: Robinson, R]]
	[[Category: Ghadessy, F]]		[[Category: Ghadessy, F]]
		+	[[Category: Robinson, R]]

OCA: Protected "6im7" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2018 05:20:43 GMT

Protected "6im7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 31 October 2018

OCA: New page: '''Unreleased structure''' The entry 6im7 is ON HOLD Authors: Wongsantichon, J., Robinson, R., Ghadessy, F. Description: CueO-12.1 multicopper oxidase [[Category: Unreleased Structures...

OCA — Wed, 31 Oct 2018 05:20:42 GMT

New page: '''Unreleased structure''' The entry 6im7 is ON HOLD Authors: Wongsantichon, J., Robinson, R., Ghadessy, F. Description: CueO-12.1 multicopper oxidase [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 6im7 is ON HOLD

Authors: Wongsantichon, J., Robinson, R., Ghadessy, F.

Description: CueO-12.1 multicopper oxidase
[[Category: Unreleased Structures]]
[[Category: Wongsantichon, J]]
[[Category: Robinson, R]]
[[Category: Ghadessy, F]]