6inc - Revision history

OCA at 09:46, 22 November 2023

OCA — Wed, 22 Nov 2023 09:46:30 GMT

←Older revision		Revision as of 09:46, 22 November 2023
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	==Crystal structure of an acetolactate decarboxylase from Klebsiella pneumoniae==		==Crystal structure of an acetolactate decarboxylase from Klebsiella pneumoniae==
-	<StructureSection load='6inc' size='340' side='right' caption='[[6inc]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='6inc' size='340' side='right'caption='[[6inc]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6inc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6INC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6INC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6inc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6INC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6INC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.604Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_decarboxylase Acetolactate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.5 4.1.1.5] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6inc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6inc OCA], [~~http~~://pdbe.org/6inc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6inc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6inc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6inc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6inc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6inc OCA], [https://pdbe.org/6inc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6inc RCSB], [https://www.ebi.ac.uk/pdbsum/6inc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6inc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/W9BHF3_KLEPN W9BHF3_KLEPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acetolactate decarboxylase~~]]	+	[[Category: Klebsiella pneumoniae]]
-	[[Category: ~~Bartlam, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wu, W~~]]	+	[[Category: Bartlam M]]
-	[[Category: ~~Zhang, Q~~]]	+	[[Category: Wu W]]
-	[[Category: ~~Unknown function~~]]	+	[[Category: Zhang Q]]

OCA at 12:11, 16 January 2019

OCA — Wed, 16 Jan 2019 12:11:55 GMT

←Older revision		Revision as of 12:11, 16 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6inc is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of an acetolactate decarboxylase from Klebsiella pneumoniae==
		+	<StructureSection load='6inc' size='340' side='right' caption='[[6inc]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6inc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6INC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6INC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_decarboxylase Acetolactate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.5 4.1.1.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6inc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6inc OCA], [http://pdbe.org/6inc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6inc RCSB], [http://www.ebi.ac.uk/pdbsum/6inc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6inc ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetolactate decarboxylase (ALDC) is a well-characterized anabolic enzyme involved with 3-hydroxy butanone (acetoin), an important physiological metabolite excreted by microbes. Although the enzyme is widely present in microorganisms, few atomic structures and functions of ALDC have been reported to date. Here we report the crystal structure of ALDC from Klebsiella pneumoniae KP (KpALDC). KpALDC crystallizes in space group P3121 with one monomer per asymmetric unit. Analytical ultracentrifugation data shows that KpALDC forms a stable dimer but can exist as a tetramer in solution. A Zn(2+) ion is coordinated by three strictly-conserved histidines (His198, His200 and His211) and a conserved glutamate (Glu69), but the C-terminal tail that forms part of the active site in ALDC enzymes is disordered. A complex structure with ethane-1,2-diol shows a unusual mode of binding, whereby the ligand does not coordinate the Zn(2+) ion. MicroScale Thermophoresis analysis shows that KpALDC binds Zn(2+) ions, but no binding of Mg(2+), Ca(2+) and Mn(2+) ions was detected.

-	~~Authors~~:	+	Structural characterization of an acetolactate decarboxylase from Klebsiella pneumoniae.,Wu W, Zhao Q, Che S, Jia H, Liang H, Zhang H, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2019 Jan 29;509(1):154-160. doi:, 10.1016/j.bbrc.2018.12.094. Epub 2018 Dec 21. PMID:30580999<ref>PMID:30580999</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6inc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acetolactate decarboxylase]]
		+	[[Category: Bartlam, M]]
		+	[[Category: Wu, W]]
		+	[[Category: Zhang, Q]]
		+	[[Category: Unknown function]]

OCA at 05:07, 5 December 2018

OCA — Wed, 05 Dec 2018 05:07:09 GMT

←Older revision		Revision as of 05:07, 5 December 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6inc is ON HOLD	+	The entry 6inc is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6inc" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2018 05:24:02 GMT

Protected "6inc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:24, 31 October 2018

OCA: New page: '''Unreleased structure''' The entry 6inc is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Oct 2018 05:24:00 GMT

New page: '''Unreleased structure''' The entry 6inc is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6inc is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]