6io6 - Revision history

OCA at 09:47, 22 November 2023

OCA — Wed, 22 Nov 2023 09:47:23 GMT

←Older revision		Revision as of 09:47, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6io6' size='340' side='right'caption='[[6io6]], [[Resolution\|resolution]] 2.64Å' scene=''>		<StructureSection load='6io6' size='340' side='right'caption='[[6io6]], [[Resolution\|resolution]] 2.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6io6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6io6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IO6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.64Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">gapA, b1779, JW1768 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [https://pdbe.org/6io6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [https://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [~~http~~://pdbe.org/6io6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G3P1_ECOLI G3P1_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sun, H]]	+	[[Category: Sun H]]
-	[[Category: Wang, H]]	+	[[Category: Wang H]]
-	[[Category: Wang, M]]	+	[[Category: Wang M]]
-	~~[[Category: Cytosolic protein]]~~	+
-	~~[[Category: Dehydrogenase]]~~	+
-	~~[[Category: Silver~~]]	+

OCA at 17:27, 20 November 2019

OCA — Wed, 20 Nov 2019 17:27:19 GMT

←Older revision		Revision as of 17:27, 20 November 2019
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [http://pdbe.org/6io6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [http://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [http://pdbe.org/6io6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [http://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Silver has long been used as an antibacterial agent, yet its molecular targets remain largely unknown. Using a custom-designed coupling of gel electrophoresis with inductively coupled plasma mass spectrometry (GE-ICP-MS), we identified six silver-binding proteins in E. coli. The majority of the identified proteins are associated with the central carbon metabolism of E. coli. Among them, we unveil that GAPDH, an essential enzyme in glycolysis, serves as a vital target of Ag(+) in E. coli for the first time. We demonstrate that silver inhibits the enzymatic function of GAPDH through targeting Cys149 in its catalytic site. The X-ray structure reveals that Ag(+) coordinates to Cys149 and His176 with a quasi-linear geometry (S-Ag-N angle of 157 degrees ). And unexpectedly, two Ag(+) ions coordinate to Cys288 in the non-catalytic site with weak argentophilic interaction (Ag...Ag distance of 2.9 A). This is the first report on antimicrobial Ag(+) targeting a key enzyme in the glycolytic pathway of E. coli. The findings expand our knowledge on the mode of action and bio-coordination chemistry of silver, particularly silver-targeting residues in proteins at the atomic level.
		+
		+	Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis of E. coli.,Wang H, Wang M, Yang X, Xu X, Hao Q, Yan A, Hu M, Lobinski R, Li H, Sun H Chem Sci. 2019 Jun 24;10(30):7193-7199. doi: 10.1039/c9sc02032b. eCollection 2019, Aug 14. PMID:31588287<ref>PMID:31588287</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6io6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures\|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]		*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures\|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:21, 14 August 2019

OCA — Wed, 14 Aug 2019 17:21:45 GMT

←Older revision		Revision as of 17:21, 14 August 2019
Line 3:		Line 3:
	<StructureSection load='6io6' size='340' side='right'caption='[[6io6]], [[Resolution\|resolution]] 2.64Å' scene=''>		<StructureSection load='6io6' size='340' side='right'caption='[[6io6]], [[Resolution\|resolution]] 2.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6io6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6io6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IO6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gapA, b1779, JW1768 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [http://pdbe.org/6io6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [http://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [http://pdbe.org/6io6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [http://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures\|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sun, H]]		[[Category: Sun, H]]

OCA at 05:50, 3 July 2019

OCA — Wed, 03 Jul 2019 05:50:16 GMT

←Older revision		Revision as of 05:50, 3 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6io6 ~~is ON HOLD until Paper Publication~~	+	==Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site==
-		+	<StructureSection load='6io6' size='340' side='right'caption='[[6io6]], [[Resolution\|resolution]] 2.64Å' scene=''>
-	~~Authors~~: ~~Wang, H~~.~~, Sun, H~~.~~, Wang, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6io6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IO6 FirstGlance]. <br>
-	~~Description~~: ~~Silver~~-~~bound~~ Glyceraldehyde-3-phosphate dehydrogenase ~~A at non~~-~~catalytic site~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] </span></td></tr>
-	[[Category: ~~Wang, M~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6io6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io6 OCA], [http://pdbe.org/6io6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6io6 RCSB], [http://www.ebi.ac.uk/pdbsum/6io6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6io6 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sun, H]]		[[Category: Sun, H]]
	[[Category: Wang, H]]		[[Category: Wang, H]]
		+	[[Category: Wang, M]]
		+	[[Category: Cytosolic protein]]
		+	[[Category: Dehydrogenase]]
		+	[[Category: Silver]]

OCA at 05:07, 5 December 2018

OCA — Wed, 05 Dec 2018 05:07:35 GMT

←Older revision		Revision as of 05:07, 5 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6io6 is ON HOLD	+	The entry 6io6 is ON HOLD until Paper Publication

	Authors: Wang, H., Sun, H., Wang, M.		Authors: Wang, H., Sun, H., Wang, M.

OCA: Protected "6io6" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:43:54 GMT

Protected "6io6" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:43, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6io6 is ON HOLD Authors: Wang, H., Sun, H., Wang, M. Description: Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site [[C...

OCA — Wed, 07 Nov 2018 10:43:53 GMT

New page: '''Unreleased structure''' The entry 6io6 is ON HOLD Authors: Wang, H., Sun, H., Wang, M. Description: Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site [[C...

New page

'''Unreleased structure'''

The entry 6io6 is ON HOLD

Authors: Wang, H., Sun, H., Wang, M.

Description: Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A at non-catalytic site
[[Category: Unreleased Structures]]
[[Category: Wang, M]]
[[Category: Sun, H]]
[[Category: Wang, H]]