6iqi - Revision history

OCA at 09:50, 22 November 2023

OCA — Wed, 22 Nov 2023 09:50:33 GMT

←Older revision		Revision as of 09:50, 22 November 2023
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	<StructureSection load='6iqi' size='340' side='right'caption='[[6iqi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6iqi' size='340' side='right'caption='[[6iqi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iqi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iqi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IQI FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PRO2 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3702 ARATH])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqi OCA], [~~http~~://pdbe.org/6iqi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iqi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iqi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqi OCA], [https://pdbe.org/6iqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iqi RCSB], [https://www.ebi.ac.uk/pdbsum/6iqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PROF2_ARATH PROF2_ARATH]~~] Binds to actin and ~~affects~~ the ~~structure~~ of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. ~~By binding~~ to ~~PIP2~~, it inhibits the ~~formation of IP3~~ and DG (By similarity).	+	[https://www.uniprot.org/uniprot/PRF2_ARATH PRF2_ARATH] Binds to actin monomers and regulates the organization of the actin cytoskeleton (PubMed:21090759). At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations (PubMed:26996265, PubMed:16313636, PubMed:29861135). At low concentrations, associates with the poly-proline motif of formins to enhance actin filament elongation rate (PubMed:29861135). Binds G-actin and poly-L-proline with low affinity in vitro (PubMed:19200149). Binds ACT1, ACT7 and ACT11 and inhibits actin polymerization (PubMed:26578694). May be involved in the cross-talk between vesicular trafficking and the actin cytoskeleton (PubMed:21090759). At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations (By similarity). Inhibits cell growth of various pathogenic fungal strains (PubMed:30056100). May play a role as antifungal proteins in the defense system against fungal pathogen attacks (PubMed:30056100).[UniProtKB:P07737]<ref>PMID:16313636</ref> <ref>PMID:19200149</ref> <ref>PMID:21090759</ref> <ref>PMID:26578694</ref> <ref>PMID:26996265</ref> <ref>PMID:29861135</ref> <ref>PMID:30056100</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao, Y]]	+	[[Category: Gao Y]]
-	[[Category: Qiao, Z]]	+	[[Category: Qiao Z]]
-	~~[[Category: Actin binding]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Regulate actin network~~]]	+

OCA at 10:46, 18 December 2019

OCA — Wed, 18 Dec 2019 10:46:35 GMT

←Older revision		Revision as of 10:46, 18 December 2019
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	<StructureSection load='6iqi' size='340' side='right'caption='[[6iqi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6iqi' size='340' side='right'caption='[[6iqi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iqi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iqi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IQI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqi OCA], [http://pdbe.org/6iqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iqi RCSB], [http://www.ebi.ac.uk/pdbsum/6iqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqi ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqi OCA], [http://pdbe.org/6iqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iqi RCSB], [http://www.ebi.ac.uk/pdbsum/6iqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 6iqi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6iqi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Profilin 3D Structures\|Profilin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]

OCA at 06:36, 6 November 2019

OCA — Wed, 06 Nov 2019 06:36:36 GMT

←Older revision		Revision as of 06:36, 6 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6iqi is ~~ON HOLD until Paper~~ Publication	+	==crystal structure of Arabidopsis thaliana Profilin 2==
		+	<StructureSection load='6iqi' size='340' side='right'caption='[[6iqi]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6iqi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IQI FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqi OCA], [http://pdbe.org/6iqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iqi RCSB], [http://www.ebi.ac.uk/pdbsum/6iqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PROF2_ARATH PROF2_ARATH]] Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Profilins are abundant cytosolic proteins that are universally expressed in eukaryotes and regulate actin filament elongation by binding to both monomeric actin (G-actin) and formin proteins. The atypical profilin Arabidopsis AtPRF3 has been reported to cooperate with canonical profilin isoforms in suppressing formin-mediated actin polymerization during plant innate immunity responses. AtPRF3 has a 37-amino-acid-long N terminal extension (NTE), and its suppressive effect on actin assembly is derived from enhanced interaction with the poly-proline (Poly-P) of the formin AtFH1. However, the molecular mechanism remains unclear. Here, we solved the crystal structures of AtPRF3Delta22 and AtPRF3Delta37, as well as AtPRF2 apo form and in complex with AtFH1 Poly-P at 1.5-3.6 A resolutions. By combining these structures with molecular modeling, we found that AtPRF3Delta22 NTE has high plasticity, with a primary "closed" conformation that can adopt an open conformation that enables Poly-P binding. Furthermore, using molecular dynamics simulation and free-energy calculations of protein-protein binding, along with experimental validation, we show that the AtPRF3Delta22 binds to Poly-P in an adaptive manner, thereby enabling different binding modes that maintain the interaction through disordered sequences. Together, our structural and simulation results suggest that the dynamic conformational changes of the AtPRF3 NTE upon Poly-P binding modulate their interactions to fine-tune formin-mediated actin assembly.

-	~~Authors: Qiao~~, Z., ~~Gao~~, Y.	+	Structural and computational examination of the Arabidopsis profilin-Poly-P complex reveals mechanistic details in profilin-regulated actin assembly.,Qiao Z, Sun H, Ng JTY, Ma Q, Koh SH, Mu Y, Miao Y, Gao YG J Biol Chem. 2019 Oct 25. pii: RA119.011307. doi: 10.1074/jbc.RA119.011307. PMID:31653702<ref>PMID:31653702</ref>

-	~~Description: crystal structure~~ of ~~Arabidopsis thaliana Profilin 2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6iqi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
	[[Category: Qiao, Z]]		[[Category: Qiao, Z]]
		+	[[Category: Actin binding]]
		+	[[Category: Plant protein]]
		+	[[Category: Regulate actin network]]

OCA at 06:22, 2 October 2019

OCA — Wed, 02 Oct 2019 06:22:58 GMT

←Older revision		Revision as of 06:22, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iqi is ON HOLD	+	The entry 6iqi is ON HOLD until Paper Publication

	Authors: Qiao, Z., Gao, Y.		Authors: Qiao, Z., Gao, Y.

OCA at 13:32, 28 August 2019

OCA — Wed, 28 Aug 2019 13:32:55 GMT

←Older revision		Revision as of 13:32, 28 August 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iqi is ON HOLD ~~until Paper Publication~~	+	The entry 6iqi is ON HOLD

	Authors: Qiao, Z., Gao, Y.		Authors: Qiao, Z., Gao, Y.

OCA at 18:52, 2 December 2018

OCA — Sun, 02 Dec 2018 18:52:07 GMT

←Older revision		Revision as of 18:52, 2 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iqi is ON HOLD	+	The entry 6iqi is ON HOLD until Paper Publication

	Authors: Qiao, Z., Gao, Y.		Authors: Qiao, Z., Gao, Y.

OCA: Protected "6iqi" [edit=sysop:move=sysop]

OCA — Wed, 14 Nov 2018 06:33:48 GMT

Protected "6iqi" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:33, 14 November 2018

OCA: New page: '''Unreleased structure''' The entry 6iqi is ON HOLD Authors: Qiao, Z., Gao, Y. Description: crystal structure of Arabidopsis thaliana Profilin 2 Category: Unreleased Structures [[...

OCA — Wed, 14 Nov 2018 06:33:47 GMT

New page: '''Unreleased structure''' The entry 6iqi is ON HOLD Authors: Qiao, Z., Gao, Y. Description: crystal structure of Arabidopsis thaliana Profilin 2 Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 6iqi is ON HOLD

Authors: Qiao, Z., Gao, Y.

Description: crystal structure of Arabidopsis thaliana Profilin 2
[[Category: Unreleased Structures]]
[[Category: Gao, Y]]
[[Category: Qiao, Z]]