6iqx - Revision history

OCA at 09:51, 22 November 2023

OCA — Wed, 22 Nov 2023 09:51:27 GMT

←Older revision		Revision as of 09:51, 22 November 2023
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	==High resolution structure of bilirubin oxidase from Myrothecium verrucaria - M467Q mutant, aerobically prepared==		==High resolution structure of bilirubin oxidase from Myrothecium verrucaria - M467Q mutant, aerobically prepared==
-	<StructureSection load='6iqx' size='340' side='right' caption='[[6iqx]], [[Resolution\|resolution]] 1.43Å' scene=''>	+	<StructureSection load='6iqx' size='340' side='right'caption='[[6iqx]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iqx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_9095 Atcc 9095~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IQX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iqx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Albifimbria_verrucaria Albifimbria verrucaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IQX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.432Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bilirubin_oxidase Bilirubin oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.5 1.3.3.5] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqx OCA], [~~http~~://pdbe.org/6iqx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iqx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iqx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqx OCA], [https://pdbe.org/6iqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iqx RCSB], [https://www.ebi.ac.uk/pdbsum/6iqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLRO_ALBVE BLRO_ALBVE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 9095~~]]	+	[[Category: Albifimbria verrucaria]]
-	[[Category: ~~Bilirubin oxidase~~]]	+	[[Category: Large Structures]]
-	[[Category: Akter, M]]	+	[[Category: Akter M]]
-	[[Category: Higuchi, Y]]	+	[[Category: Higuchi Y]]
-	[[Category: Shibata, N]]	+	[[Category: Shibata N]]
-	~~[[Category: Multicopper oxydase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:45, 26 December 2018

OCA — Wed, 26 Dec 2018 08:45:53 GMT

←Older revision		Revision as of 08:45, 26 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6iqx is ~~ON HOLD~~	+	==High resolution structure of bilirubin oxidase from Myrothecium verrucaria - M467Q mutant, aerobically prepared==
		+	<StructureSection load='6iqx' size='340' side='right' caption='[[6iqx]], [[Resolution\|resolution]] 1.43Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6iqx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9095 Atcc 9095]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IQX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bilirubin_oxidase Bilirubin oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.5 1.3.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iqx OCA], [http://pdbe.org/6iqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iqx RCSB], [http://www.ebi.ac.uk/pdbsum/6iqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iqx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bilirubin oxidase (BOD) belongs to the family of blue multicopper oxidases, and catalyzes the concomitant oxidation of bilirubin to biliverdin and the reduction of molecular oxygen to water via a four-electron reduction system. The active sites of BOD comprise four copper atoms; type I copper (T1Cu) forms a mononuclear site, and a cluster of three copper atoms forms a trinuclear center. In the present study, we determined the high-resolution crystal structures of BOD from the fungus Myrothecium verrucaria. We investigated wild-type (WT) BOD and a BOD mutant called Met467Gln, which is inactive against bilirubin. The structures revealed that a novel post-translational crosslink between Trp396 and His398 is formed in the vicinity of the T1Cu site in WT, whereas it is absent in the Met467Gln mutant. Our structural, computational, and electrochemical studies suggest that the His-Trp crosslink adjusts the redox potential of T1Cu to that of bilirubin to efficiently abstract electrons from the substrate.

-	~~Authors: Shibata, N~~., Akter, M., ~~Higuchi~~, Y.	+	Redox potential-dependent formation of an unusual His-Trp bond in bilirubin oxidase.,Akter M, Tokiwa T, Shoji M, Nishikawa K, Shigeta Y, Sakurai T, Higuchi Y, Kataoka K, Shibata N Chemistry. 2018 Aug 29. doi: 10.1002/chem.201803798. PMID:30156345<ref>PMID:30156345</ref>

-	~~Description: High resolution structure~~ of ~~bilirubin oxidase from Myrothecium verrucaria~~ -~~M467Q mutant, aerobically prepared~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Shibata, N~~]]	+	<div class="pdbe-citations 6iqx" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Higuchi, Y~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 9095]]
		+	[[Category: Bilirubin oxidase]]
	[[Category: Akter, M]]		[[Category: Akter, M]]
		+	[[Category: Higuchi, Y]]
		+	[[Category: Shibata, N]]
		+	[[Category: Multicopper oxydase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6iqx" [edit=sysop:move=sysop]

OCA — Wed, 14 Nov 2018 06:35:04 GMT

Protected "6iqx" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:35, 14 November 2018

OCA: New page: '''Unreleased structure''' The entry 6iqx is ON HOLD Authors: Shibata, N., Akter, M., Higuchi, Y. Description: High resolution structure of bilirubin oxidase from Myrothecium verrucari...

OCA — Wed, 14 Nov 2018 06:35:02 GMT

New page: '''Unreleased structure''' The entry 6iqx is ON HOLD Authors: Shibata, N., Akter, M., Higuchi, Y. Description: High resolution structure of bilirubin oxidase from Myrothecium verrucari...

New page

'''Unreleased structure'''

The entry 6iqx is ON HOLD

Authors: Shibata, N., Akter, M., Higuchi, Y.

Description: High resolution structure of bilirubin oxidase from Myrothecium verrucaria -M467Q mutant, aerobically prepared
[[Category: Unreleased Structures]]
[[Category: Shibata, N]]
[[Category: Higuchi, Y]]
[[Category: Akter, M]]