6is2 - Revision history

OCA at 10:34, 27 March 2024

OCA — Wed, 27 Mar 2024 10:34:58 GMT

←Older revision		Revision as of 10:34, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staab Staab~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_RF122 Staphylococcus aureus RF122]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.591Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arlR, SAB1271c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [~~http~~://pdbe.org/6is2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [https://pdbe.org/6is2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [https://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ARLR_STAAB ARLR_STAAB]] Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence.	+	[https://www.uniprot.org/uniprot/ARLR_STAAB ARLR_STAAB] Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Staphylococcus aureus ArlRS is a key two-component regulatory system necessary for adhesion, biofilm formation, and virulence. The response regulator ArlR consists of a C-terminal DNA-binding effector domain and an N-terminal receiver domain that is phosphorylated by ArlS, the cognate transmembrane sensor histidine kinase. We demonstrate that the receiver domain of ArlR adopts the canonical alpha5beta5 response regulator assembly, which dimerizes upon activation, using beryllium trifluoride as an aspartate phosphorylation mimic. Activated ArlR recognizes a 20-bp imperfect inverted repeat sequence in the ica operon, which is involved in intercellular adhesion polysaccharide production. Crystal structures of the inactive and activated forms reveal that activation induces a significant conformational change in the beta4-alpha4 and beta5-alpha5-connecting loops, in which the alpha4 and alpha5 helices constitute the homodimerization interface. Crystal structures of the DNA-binding ArlR effector domain indicate that it is able to dimerize via a non-canonical beta1-beta2 hairpin domain swapping, raising the possibility of a new mechanism for signal transduction from the receiver domain to effector domain. Taken together, the current study provides structural insights into the activation of ArlR and its recognition, adding to the diversity of response regulation mechanisms that may inspire novel antimicrobial strategies specifically targeting Staphylococcus.	+
-		+
-	Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR.,Ouyang Z, Zheng F, Chew JY, Pei Y, Zhou J, Wen K, Han M, Lemieux MJ, Hwang PM, Wen Y Nucleic Acids Res. 2019 Dec 2;47(21):11418-11429. doi: 10.1093/nar/gkz891. PMID:31598698<ref>PMID:31598698</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6is2" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Response regulator 3D structure\|Response regulator 3D structure]]		*[[Response regulator 3D structure\|Response regulator 3D structure]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staab~~]]	+	[[Category: Staphylococcus aureus RF122]]
-	[[Category: Ouyang, Z]]	+	[[Category: Ouyang Z]]
-	[[Category: Wen, Y]]	+	[[Category: Wen Y]]
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Two-component regulatory system~~]]	+

OCA at 05:43, 13 May 2020

OCA — Wed, 13 May 2020 05:43:40 GMT

←Older revision		Revision as of 05:43, 13 May 2020
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	<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arlR, SAB1271c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arlR, SAB1271c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [http://pdbe.org/6is2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [http://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [http://pdbe.org/6is2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [http://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ARLR_STAAB ARLR_STAAB]] Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence.		[[http://www.uniprot.org/uniprot/ARLR_STAAB ARLR_STAAB]] Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus ArlRS is a key two-component regulatory system necessary for adhesion, biofilm formation, and virulence. The response regulator ArlR consists of a C-terminal DNA-binding effector domain and an N-terminal receiver domain that is phosphorylated by ArlS, the cognate transmembrane sensor histidine kinase. We demonstrate that the receiver domain of ArlR adopts the canonical alpha5beta5 response regulator assembly, which dimerizes upon activation, using beryllium trifluoride as an aspartate phosphorylation mimic. Activated ArlR recognizes a 20-bp imperfect inverted repeat sequence in the ica operon, which is involved in intercellular adhesion polysaccharide production. Crystal structures of the inactive and activated forms reveal that activation induces a significant conformational change in the beta4-alpha4 and beta5-alpha5-connecting loops, in which the alpha4 and alpha5 helices constitute the homodimerization interface. Crystal structures of the DNA-binding ArlR effector domain indicate that it is able to dimerize via a non-canonical beta1-beta2 hairpin domain swapping, raising the possibility of a new mechanism for signal transduction from the receiver domain to effector domain. Taken together, the current study provides structural insights into the activation of ArlR and its recognition, adding to the diversity of response regulation mechanisms that may inspire novel antimicrobial strategies specifically targeting Staphylococcus.
		+
		+	Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR.,Ouyang Z, Zheng F, Chew JY, Pei Y, Zhou J, Wen K, Han M, Lemieux MJ, Hwang PM, Wen Y Nucleic Acids Res. 2019 Dec 2;47(21):11418-11429. doi: 10.1093/nar/gkz891. PMID:31598698<ref>PMID:31598698</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6is2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Response regulator 3D structure\|Response regulator 3D structure]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:59, 6 November 2019

OCA — Wed, 06 Nov 2019 07:59:47 GMT

←Older revision		Revision as of 07:59, 6 November 2019
Line 3:		Line 3:
	<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arlR, SAB1271c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [http://pdbe.org/6is2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [http://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [http://pdbe.org/6is2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [http://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>
	</table>		</table>
Line 12:		Line 13:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staab]]
	[[Category: Ouyang, Z]]		[[Category: Ouyang, Z]]
	[[Category: Wen, Y]]		[[Category: Wen, Y]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Two-component regulatory system]]		[[Category: Two-component regulatory system]]

OCA at 09:00, 30 October 2019

OCA — Wed, 30 Oct 2019 09:00:06 GMT

←Older revision		Revision as of 09:00, 30 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6is2 is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg==
-		+	<StructureSection load='6is2' size='340' side='right'caption='[[6is2]], [[Resolution\|resolution]] 1.59Å' scene=''>
-	~~Authors~~: ~~Wen~~, Y., ~~Ouyang~~, Z.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6is2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IS2 FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure~~ of ~~Staphylococcus aureus response regulator~~ ArlR ~~receiver domain~~ in ~~complex with Mg~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6is2 OCA], [http://pdbe.org/6is2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6is2 RCSB], [http://www.ebi.ac.uk/pdbsum/6is2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6is2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARLR_STAAB ARLR_STAAB]] Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ouyang, Z]]
	[[Category: Wen, Y]]		[[Category: Wen, Y]]
-	[[Category: ~~Ouyang, Z~~]]	+	[[Category: Signaling protein]]
		+	[[Category: Two-component regulatory system]]

OCA at 06:36, 26 December 2018

OCA — Wed, 26 Dec 2018 06:36:07 GMT

←Older revision		Revision as of 06:36, 26 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6is2 is ON HOLD	+	The entry 6is2 is ON HOLD until Paper Publication

	Authors: Wen, Y., Ouyang, Z.		Authors: Wen, Y., Ouyang, Z.
Line 7:		Line 7:
	Description: Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg		Description: Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ouyang, Z]]
	[[Category: Wen, Y]]		[[Category: Wen, Y]]
		+	[[Category: Ouyang, Z]]

OCA: Protected "6is2" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 18:54:46 GMT

Protected "6is2" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:54, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6is2 is ON HOLD Authors: Wen, Y., Ouyang, Z. Description: Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in com...

OCA — Sun, 02 Dec 2018 18:54:45 GMT

New page: '''Unreleased structure''' The entry 6is2 is ON HOLD Authors: Wen, Y., Ouyang, Z. Description: Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in com...

New page

'''Unreleased structure'''

The entry 6is2 is ON HOLD

Authors: Wen, Y., Ouyang, Z.

Description: Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg
[[Category: Unreleased Structures]]
[[Category: Ouyang, Z]]
[[Category: Wen, Y]]