6iv1 - Revision history

OCA at 19:26, 29 May 2024

OCA — Wed, 29 May 2024 19:26:34 GMT

←Older revision		Revision as of 19:26, 29 May 2024
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	<StructureSection load='6iv1' size='340' side='right'caption='[[6iv1]], [[Resolution\|resolution]] 3.18Å' scene=''>		<StructureSection load='6iv1' size='340' side='right'caption='[[6iv1]], [[Resolution\|resolution]] 3.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iv1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae_is53~~ Klebsiella pneumoniae ~~is53~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IV1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iv1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_IS53 Klebsiella pneumoniae IS53]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IV1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.18Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[4wd8\|4wd8]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iv1 OCA], [https://pdbe.org/6iv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iv1 RCSB], [https://www.ebi.ac.uk/pdbsum/6iv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iv1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iv1 OCA], [https://pdbe.org/6iv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iv1 RCSB], [https://www.ebi.ac.uk/pdbsum/6iv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iv1 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6iv1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6iv1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bestrophin 3D structures\|Bestrophin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Klebsiella pneumoniae ~~is53~~]]	+	[[Category: Klebsiella pneumoniae IS53]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, S]]	+	[[Category: Chen S]]
-	[[Category: Kittredge, A]]	+	[[Category: Kittredge A]]
-	[[Category: Yang, T]]	+	[[Category: Yang T]]
-	~~[[Category: Bestrophin-1]]~~	+
-	~~[[Category: Homolog]]~~	+
-	~~[[Category: Klebsiella pneumoniae]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Mutation~~]]	+

OCA at 05:32, 8 June 2022

OCA — Wed, 08 Jun 2022 05:32:50 GMT

←Older revision		Revision as of 05:32, 8 June 2022
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	<StructureSection load='6iv1' size='340' side='right'caption='[[6iv1]], [[Resolution\|resolution]] 3.18Å' scene=''>		<StructureSection load='6iv1' size='340' side='right'caption='[[6iv1]], [[Resolution\|resolution]] 3.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iv1]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IV1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IV1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iv1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_is53 Klebsiella pneumoniae is53]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IV1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wd8\|4wd8]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4wd8\|4wd8]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iv1 OCA], [~~http~~://pdbe.org/6iv1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iv1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iv1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iv1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iv1 OCA], [https://pdbe.org/6iv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iv1 RCSB], [https://www.ebi.ac.uk/pdbsum/6iv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iv1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae is53]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen, S]]		[[Category: Chen, S]]
		+	[[Category: Kittredge, A]]
		+	[[Category: Yang, T]]
	[[Category: Bestrophin-1]]		[[Category: Bestrophin-1]]
	[[Category: Homolog]]		[[Category: Homolog]]

OCA at 10:58, 13 November 2019

OCA — Wed, 13 Nov 2019 10:58:25 GMT

←Older revision		Revision as of 10:58, 13 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6iv1 is ~~ON HOLD until Dec 02 2020~~	+	==Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180T==
		+	<StructureSection load='6iv1' size='340' side='right'caption='[[6iv1]], [[Resolution\|resolution]] 3.18Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6iv1]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IV1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wd8\|4wd8]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iv1 OCA], [http://pdbe.org/6iv1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iv1 RCSB], [http://www.ebi.ac.uk/pdbsum/6iv1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iv1 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mutations of human BEST1, encoding a Ca(2+)-activated Cl(-) channel (hBest1), cause macular degenerative disorders. Best1 homolog structures reveal an evolutionarily conserved channel architecture highlighted by two landmark restrictions (named the "neck" and "aperture", respectively) in the ion conducting pathway, suggesting a unique dual-switch gating mechanism, which, however, has not been characterized well. Using patch clamp and crystallography, we demonstrate that both the neck and aperture in hBest1 are Ca(2+)-dependent gates essential for preventing channel leakage resulting from Ca(2+)-independent, spontaneous gate opening. Importantly, three patient-derived mutations (D203A, I205T and Y236C) lead to Ca(2+)-independent leakage and elevated Ca(2+)-dependent anion currents due to enhanced opening of the gates. Moreover, we identify a network of residues critically involved in gate operation. Together, our results suggest an indispensable role of the neck and aperture of hBest1 for channel gating, and uncover disease-causing mechanisms of hBest1 gain-of-function mutations.

-	~~Authors: Chen~~, S.	+	Dual Ca(2+)-dependent gates in human Bestrophin1 underlie disease-causing mechanisms of gain-of-function mutations.,Ji C, Kittredge A, Hopiavuori A, Ward N, Chen S, Fukuda Y, Zhang Y, Yang T Commun Biol. 2019 Jun 24;2:240. doi: 10.1038/s42003-019-0433-3. eCollection 2019. PMID:31263784<ref>PMID:31263784</ref>

-	~~Description: Crystal structure of~~ a ~~bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180T~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6iv1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chen, S]]		[[Category: Chen, S]]
		+	[[Category: Bestrophin-1]]
		+	[[Category: Homolog]]
		+	[[Category: Klebsiella pneumoniae]]
		+	[[Category: Membrane protein]]
		+	[[Category: Mutation]]

OCA at 05:21, 6 March 2019

OCA — Wed, 06 Mar 2019 05:21:54 GMT

←Older revision		Revision as of 05:21, 6 March 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iv1 is ON HOLD	+	The entry 6iv1 is ON HOLD until Dec 02 2020

	Authors: Chen, S.		Authors: Chen, S.

OCA: Protected "6iv1" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:11:02 GMT

Protected "6iv1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6iv1 is ON HOLD Authors: Chen, S. Description: Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180T...

OCA — Wed, 12 Dec 2018 05:11:00 GMT

New page: '''Unreleased structure''' The entry 6iv1 is ON HOLD Authors: Chen, S. Description: Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180T...

New page

'''Unreleased structure'''

The entry 6iv1 is ON HOLD

Authors: Chen, S.

Description: Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180T
[[Category: Unreleased Structures]]
[[Category: Chen, S]]