6iwa - Revision history

OCA at 09:54, 22 November 2023

OCA — Wed, 22 Nov 2023 09:54:09 GMT

←Older revision		Revision as of 09:54, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6iwa' size='340' side='right'caption='[[6iwa]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6iwa' size='340' side='right'caption='[[6iwa]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iwa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IWA FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwa OCA], [~~http~~://pdbe.org/6iwa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iwa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iwa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwa ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwa OCA], [https://pdbe.org/6iwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iwa RCSB], [https://www.ebi.ac.uk/pdbsum/6iwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GOGA2_HUMAN GOGA2_HUMAN]] Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).[UniProtKB:Q62839][UniProtKB:Q921M4]<ref>PMID:16489344</ref> <ref>PMID:17314401</ref> <ref>PMID:18045989</ref> <ref>PMID:19109421</ref> <ref>PMID:19242490</ref> <ref>PMID:26165940</ref> [[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.	+	[https://www.uniprot.org/uniprot/GOGA2_HUMAN GOGA2_HUMAN] Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).[UniProtKB:Q62839][UniProtKB:Q921M4]<ref>PMID:16489344</ref> <ref>PMID:17314401</ref> <ref>PMID:18045989</ref> <ref>PMID:19109421</ref> <ref>PMID:19242490</ref> <ref>PMID:26165940</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6iwa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6iwa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Importin 3D structures\|Importin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang, C C]]	+	[[Category: Mus musculus]]
-	[[Category: Chen, C J]]	+	[[Category: Chang C-C]]
-	[[Category: Hsia, K C]]	+	[[Category: Chen C-J]]
-	[[Category: Pien, Y C]]	+	[[Category: Hsia K-C]]
-	[[Category: Tsai, S Y]]	+	[[Category: Pien Y-C]]
-	~~[[Category: Gm130]]~~	+	[[Category: Tsai S-Y]]
-	~~[[Category: Importin alpha]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 10:21, 2 October 2019

OCA — Wed, 02 Oct 2019 10:21:03 GMT

←Older revision		Revision as of 10:21, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6iwa is ~~ON HOLD~~	+	==Crystal structure of Importin-alpha and phosphoserine GM130==
		+	<StructureSection load='6iwa' size='340' side='right'caption='[[6iwa]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6iwa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IWA FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwa OCA], [http://pdbe.org/6iwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iwa RCSB], [http://www.ebi.ac.uk/pdbsum/6iwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwa ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GOGA2_HUMAN GOGA2_HUMAN]] Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).[UniProtKB:Q62839][UniProtKB:Q921M4]<ref>PMID:16489344</ref> <ref>PMID:17314401</ref> <ref>PMID:18045989</ref> <ref>PMID:19109421</ref> <ref>PMID:19242490</ref> <ref>PMID:26165940</ref> [[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To facilitate proper mitotic cell partitioning, the Golgi disassembles by suppressing vesicle fusion. However, the underlying mechanism has not been characterized previously. Here, we report a Ran pathway-independent attenuation mechanism that allows Importin-alpha (a nuclear transport factor) to suppress the vesicle fusion mediated by p115 (a vesicular tethering factor) and is required for mitotic Golgi disassembly. We demonstrate that Importin-alpha directly competes with p115 for interaction with the Golgi protein GM130. This interaction, promoted by a phosphate moiety on GM130, is independent of Importin-beta and Ran. A GM130 K34A mutant, in which the Importin-alpha-GM130 interaction is specifically disrupted, exhibited abundant Golgi puncta during metaphase. Importantly, a mutant showing enhanced p115-GM130 interaction presented proliferative defects and G2/M arrest, demonstrating that Importin-alpha-GM130 binding modulates the Golgi disassembly that governs mitotic progression. Our findings illuminate that the Ran and kinase-phosphatase pathways regulate multiple aspects of mitosis coordinated by Importin-alpha (e.g. spindle assembly, Golgi disassembly).

-	~~Authors: Chang, C.~~-C., Chen, C~~.-J.~~, Pien, ~~Y.-~~C., Tsai, S.-Y.~~, Hsia, K~~.-C.	+	Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-alpha.,Chang CC, Chen CJ, Grauffel C, Pien YC, Lim C, Tsai SY, Hsia KC Nat Commun. 2019 Sep 20;10(1):4307. doi: 10.1038/s41467-019-12207-4. PMID:31541088<ref>PMID:31541088</ref>

-	~~Description: Crystal structure~~ of ~~Importin~~-~~alpha and phosphoserine GM130~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Chang, C.-C]]	+	<div class="pdbe-citations 6iwa" style="background-color:#fffaf0;"></div>
-	[[Category: Chen, C.-J]]	+	== References ==
-	[[Category: ~~Tsai~~, ~~S.-Y~~]]	+	<references/>
-	[[Category: ~~Hsia~~, ~~K.-~~C]]	+	__TOC__
-	[[Category: ~~Pien~~, Y~~.-C~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang, C C]]
		+	[[Category: Chen, C J]]
		+	[[Category: Hsia, K C]]
		+	[[Category: Pien, Y C]]
		+	[[Category: Tsai, S Y]]
		+	[[Category: Gm130]]
		+	[[Category: Importin alpha]]
		+	[[Category: Protein binding]]

OCA: Protected "6iwa" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:14:59 GMT

Protected "6iwa" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6iwa is ON HOLD Authors: Chang, C.-C., Chen, C.-J., Pien, Y.-C., Tsai, S.-Y., Hsia, K.-C. Description: Crystal structure of Importin-alpha and phos...

OCA — Wed, 12 Dec 2018 05:14:57 GMT

New page: '''Unreleased structure''' The entry 6iwa is ON HOLD Authors: Chang, C.-C., Chen, C.-J., Pien, Y.-C., Tsai, S.-Y., Hsia, K.-C. Description: Crystal structure of Importin-alpha and phos...

New page

'''Unreleased structure'''

The entry 6iwa is ON HOLD

Authors: Chang, C.-C., Chen, C.-J., Pien, Y.-C., Tsai, S.-Y., Hsia, K.-C.

Description: Crystal structure of Importin-alpha and phosphoserine GM130
[[Category: Unreleased Structures]]
[[Category: Chang, C.-C]]
[[Category: Chen, C.-J]]
[[Category: Tsai, S.-Y]]
[[Category: Hsia, K.-C]]
[[Category: Pien, Y.-C]]