6iwk - Revision history

OCA at 10:39, 31 March 2021

OCA — Wed, 31 Mar 2021 10:39:14 GMT

←Older revision		Revision as of 10:39, 31 March 2021
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	<StructureSection load='6iwk' size='340' side='right'caption='[[6iwk]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6iwk' size='340' side='right'caption='[[6iwk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iwk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iwk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15946 Atcc 15946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IWK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] </span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mta ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=304 ATCC 15946])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwk OCA], [~~http~~://pdbe.org/6iwk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iwk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iwk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwk ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwk OCA], [https://pdbe.org/6iwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iwk RCSB], [https://www.ebi.ac.uk/pdbsum/6iwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwk ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The maltooligosaccharide-forming amylases (MFAses) degrade starch into maltooligosaccharides which potentially benefit human diet and grow popular in food processing, but little has been studied about their product specificity and structures. We focused on this topic and provide evidence through an X-ray crystal structure of the maltotetraose (G4)-forming amylase from Pseudomonas saccharophila STB07 (MFAps), as well as co-crystal structures of MFAps with G4 and with pseudo-maltoheptaose (pseudo-G7) determined at up to 1.1 A resolution. G4 and pseudo-G7 occupy active cleft subsites -4 to -1 and -4 to +3 respectively. Binding induces conformational changes in the active sites except Asp193, working as the base catalyst. Comparison of the MFAps structure with those of other alpha-amylases revealed obvious differences in the loop structures providing dominant interactions between protein and substrate in the non-reducing side of the active sites cleft. These structures at the non-reducing end may govern the G4 specificity of MFAps and also be relevant to its exo-type action pattern.
		+
		+	Structure of maltotetraose-forming amylase from Pseudomonas saccharophila STB07 provides insights into its product specificity.,Zhang Z, Jin T, Xie X, Ban X, Li C, Hong Y, Cheng L, Gu Z, Li Z Int J Biol Macromol. 2019 Nov 18. pii: S0141-8130(19)37071-0. doi:, 10.1016/j.ijbiomac.2019.11.006. PMID:31751711<ref>PMID:31751711</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6iwk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 15946]]
	[[Category: Glucan 1,4-alpha-maltotetraohydrolase]]		[[Category: Glucan 1,4-alpha-maltotetraohydrolase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 15:02, 11 December 2019

OCA — Wed, 11 Dec 2019 15:02:50 GMT

←Older revision		Revision as of 15:02, 11 December 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6iwk ~~is ON HOLD until Paper Publication~~	+	==The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07==
-		+	<StructureSection load='6iwk' size='340' side='right'caption='[[6iwk]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors~~: ~~Li, Z~~.F.~~, Ban, X~~.F., ~~Zhang, Z~~.Q., Li, C.M., ~~Gu, Z~~.B., ~~Jin~~, T.C., ~~Li, Y~~.L., ~~Shang, Y~~.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6iwk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IWK FirstGlance]. <br>
-	~~Description~~: ~~The Structure of Maltooligosaccharide~~-~~forming Amylase from Pseudomonas saccharophila STB07~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] </span></td></tr>
-	[[Category: Gu, Z.B]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iwk OCA], [http://pdbe.org/6iwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iwk RCSB], [http://www.ebi.ac.uk/pdbsum/6iwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iwk ProSAT]</span></td></tr>
-	[[Category: ~~Ban~~, ~~X.F~~]]	+	</table>
-	[[Category: ~~Zhang~~, ~~Z.Q~~]]	+	__TOC__
-	[[Category: Li, Y.L]]	+	</StructureSection>
-	[[Category: ~~Jin~~, ~~T.C~~]]	+	[[Category: Glucan 1,4-alpha-maltotetraohydrolase]]
-	[[Category: Shang, Y.H]]	+	[[Category: Large Structures]]
-	[[Category: Li, ~~C.M~~]]	+	[[Category: Ban, X F]]
-	[[Category: ~~Li, Z.F~~]]	+	[[Category: Gu, Z B]]
		+	[[Category: Jin, T C]]
		+	[[Category: Li, C M]]
		+	[[Category: Li, Y L]]
		+	[[Category: Li, Z F]]
		+	[[Category: Shang, Y H]]
		+	[[Category: Zhang, Z Q]]
		+	[[Category: Maltooligosaccharide-forming amyla]]
		+	[[Category: Pseudomonas saccharophila stb07]]
		+	[[Category: Sugar binding protein]]

OCA at 09:56, 13 March 2019

OCA — Wed, 13 Mar 2019 09:56:08 GMT

←Older revision		Revision as of 09:56, 13 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iwk is ON HOLD	+	The entry 6iwk is ON HOLD until Paper Publication

	Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H.		Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H.
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	Description: The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07		Description: The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Shang, Y.H]]
	[[Category: Gu, Z.B]]		[[Category: Gu, Z.B]]
	[[Category: Ban, X.F]]		[[Category: Ban, X.F]]
-	[[Category: Li, C.M]]
	[[Category: Zhang, Z.Q]]		[[Category: Zhang, Z.Q]]
-	[[Category: Li, Z.F]]
	[[Category: Li, Y.L]]		[[Category: Li, Y.L]]
	[[Category: Jin, T.C]]		[[Category: Jin, T.C]]
		+	[[Category: Shang, Y.H]]
		+	[[Category: Li, C.M]]
		+	[[Category: Li, Z.F]]

OCA: Protected "6iwk" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:15:32 GMT

Protected "6iwk" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6iwk is ON HOLD Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H. Description: The Structure of Malto...

OCA — Wed, 12 Dec 2018 05:15:31 GMT

New page: '''Unreleased structure''' The entry 6iwk is ON HOLD Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H. Description: The Structure of Malto...

New page

'''Unreleased structure'''

The entry 6iwk is ON HOLD

Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H.

Description: The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07
[[Category: Unreleased Structures]]
[[Category: Shang, Y.H]]
[[Category: Gu, Z.B]]
[[Category: Ban, X.F]]
[[Category: Li, C.M]]
[[Category: Zhang, Z.Q]]
[[Category: Li, Z.F]]
[[Category: Li, Y.L]]
[[Category: Jin, T.C]]