6izs - Revision history

OCA at 09:56, 22 November 2023

OCA — Wed, 22 Nov 2023 09:56:33 GMT

←Older revision		Revision as of 09:56, 22 November 2023
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	<StructureSection load='6izs' size='340' side='right'caption='[[6izs]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='6izs' size='340' side='right'caption='[[6izs]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6izs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium~~ influenzae~~" lehmann and neumann 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IZS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6izs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IZS FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bamA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~727~~ "~~Bacterium influenzae~~" ~~Lehmann and Neumann 1896~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.03Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6izs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izs OCA], [~~http~~://pdbe.org/6izs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6izs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6izs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6izs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6izs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izs OCA], [https://pdbe.org/6izs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6izs RCSB], [https://www.ebi.ac.uk/pdbsum/6izs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6izs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~BAMA1_HAEIF BAMA1_HAEIF]~~] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.[HAMAP-Rule:MF_01430]	+	[https://www.uniprot.org/uniprot/BAMA_HAEIN BAMA_HAEIN] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.[HAMAP-Rule:MF_01430]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6izs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6izs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bam complex 3D structures\|Bam complex 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ influenzae ~~lehmann and neumann 1896~~]]	+	[[Category: Haemophilus influenzae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dong, X]]	+	[[Category: Dong X]]
-	[[Category: Hong, L]]	+	[[Category: Hong L]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Ma, X]]	+	[[Category: Ma X]]
-	[[Category: Meng, G]]	+	[[Category: Meng G]]
-	[[Category: Tan, P]]	+	[[Category: Tan P]]
-	[[Category: Wang, P]]	+	[[Category: Wang P]]
-	[[Category: Wang, Q]]	+	[[Category: Wang Q]]
-	[[Category: Wu, H]]	+	[[Category: Wu H]]
-	~~[[Category: Beta-barrel assembly machinery]]~~	+
-	~~[[Category: Lipo-partner]]~~	+
-	~~[[Category: Omp recruitment]]~~	+
-	~~[[Category: Poly-potra]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:43, 27 November 2019

OCA — Wed, 27 Nov 2019 08:43:09 GMT

←Older revision		Revision as of 08:43, 27 November 2019
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	<StructureSection load='6izs' size='340' side='right'caption='[[6izs]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='6izs' size='340' side='right'caption='[[6izs]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6izs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IZS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6izs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IZS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6izs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izs OCA], [http://pdbe.org/6izs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6izs RCSB], [http://www.ebi.ac.uk/pdbsum/6izs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6izs ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bamA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6izs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izs OCA], [http://pdbe.org/6izs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6izs RCSB], [http://www.ebi.ac.uk/pdbsum/6izs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6izs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BAMA1_HAEIF BAMA1_HAEIF]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.[HAMAP-Rule:MF_01430]		[[http://www.uniprot.org/uniprot/BAMA1_HAEIF BAMA1_HAEIF]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.[HAMAP-Rule:MF_01430]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Almost all the outer membrane proteins (OMPs) fold into an invariant beta-barrel fold via the polypeptide-transport-associated (POTRA) motif and beta-barrel assembly machinery (BAM). However, whether and how poly-POTRAs interact with OMPs remain largely unknown. Here, we have characterized the structures of Haemophilus influenzae poly-POTRAs via X-ray crystallography, small angle X-ray scattering, and molecular dynamics simulation. Unexpectedly, crystal packing reveals a putative OMP travel pathway spiraled by the conserved alpha2-beta2 edges in poly-POTRAs. Supportively, the structure-based mutations targeting the OMP binding sites significantly disrupt OMP biogenesis, resulting in severe cell growth defects. Another notable feature in H. influenzae POTRA structures is flexibility. As characterized by ELISA assays, poly-POTRAs could recruit OMP substrates in a step-wise manner. More importantly, the restriction of POTRA-POTRA linkage and flexibility significantly impairs the BamA function and causes cell growth defect. Altogether, these results suggest that the beta-strand augmentations and intrinsic flexibility are important factors for BamA-OMP recruitment.-Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G. How BamA recruits OMP substrates via poly-POTRAs domain.
		+
		+	How BamA recruits OMP substrates via poly-POTRAs domain.,Ma X, Wang Q, Li Y, Tan P, Wu H, Wang P, Dong X, Hong L, Meng G FASEB J. 2019 Nov 8:fj201900681RR. doi: 10.1096/fj.201900681RR. PMID:31702961<ref>PMID:31702961</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6izs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium influenzae lehmann and neumann 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Dong, X]]		[[Category: Dong, X]]

OCA at 09:00, 30 October 2019

OCA — Wed, 30 Oct 2019 09:00:37 GMT

←Older revision		Revision as of 09:00, 30 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6izs ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Haemophilus influenzae BamA POTRA4==
-		+	<StructureSection load='6izs' size='340' side='right'caption='[[6izs]], [[Resolution\|resolution]] 2.03Å' scene=''>
-	~~Authors~~: ~~Ma, X~~.~~, Wang, Q~~.~~, Li, Y~~.~~, Tan, P~~., ~~Wu, H~~., ~~Wang, P~~., ~~Dong, X~~., ~~Hong, L~~., ~~Meng, G~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6izs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IZS FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~Haemophilus influenzae BamA POTRA4~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6izs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izs OCA], [http://pdbe.org/6izs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6izs RCSB], [http://www.ebi.ac.uk/pdbsum/6izs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6izs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BAMA1_HAEIF BAMA1_HAEIF]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.[HAMAP-Rule:MF_01430]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Dong, X]]
	[[Category: Hong, L]]		[[Category: Hong, L]]
	[[Category: Li, Y]]		[[Category: Li, Y]]
-	[[Category: ~~Wang~~, Q]]	+	[[Category: Ma, X]]
	[[Category: Meng, G]]		[[Category: Meng, G]]
	[[Category: Tan, P]]		[[Category: Tan, P]]
-	[[Category: Dong, X]]
	[[Category: Wang, P]]		[[Category: Wang, P]]
		+	[[Category: Wang, Q]]
	[[Category: Wu, H]]		[[Category: Wu, H]]
-	[[Category: ~~Ma, X~~]]	+	[[Category: Beta-barrel assembly machinery]]
		+	[[Category: Lipo-partner]]
		+	[[Category: Omp recruitment]]
		+	[[Category: Poly-potra]]
		+	[[Category: Transport protein]]

OCA at 06:03, 30 January 2019

OCA — Wed, 30 Jan 2019 06:03:15 GMT

←Older revision		Revision as of 06:03, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6izs is ON HOLD	+	The entry 6izs is ON HOLD until Paper Publication

	Authors: Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G.		Authors: Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G.
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	Description: Crystal structure of Haemophilus influenzae BamA POTRA4		Description: Crystal structure of Haemophilus influenzae BamA POTRA4
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hong, L]]
		+	[[Category: Li, Y]]
	[[Category: Wang, Q]]		[[Category: Wang, Q]]
	[[Category: Meng, G]]		[[Category: Meng, G]]
	[[Category: Tan, P]]		[[Category: Tan, P]]
-	[[Category: Hong, L]]
	[[Category: Dong, X]]		[[Category: Dong, X]]
-	[[Category: Li, Y]]
	[[Category: Wang, P]]		[[Category: Wang, P]]
	[[Category: Wu, H]]		[[Category: Wu, H]]
	[[Category: Ma, X]]		[[Category: Ma, X]]

OCA: Protected "6izs" [edit=sysop:move=sysop]

OCA — Wed, 26 Dec 2018 06:42:10 GMT

Protected "6izs" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 26 December 2018

OCA: New page: '''Unreleased structure''' The entry 6izs is ON HOLD Authors: Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G. Description: Crystal structure of Haemop...

OCA — Wed, 26 Dec 2018 06:42:08 GMT

New page: '''Unreleased structure''' The entry 6izs is ON HOLD Authors: Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G. Description: Crystal structure of Haemop...

New page

'''Unreleased structure'''

The entry 6izs is ON HOLD

Authors: Ma, X., Wang, Q., Li, Y., Tan, P., Wu, H., Wang, P., Dong, X., Hong, L., Meng, G.

Description: Crystal structure of Haemophilus influenzae BamA POTRA4
[[Category: Unreleased Structures]]
[[Category: Wang, Q]]
[[Category: Meng, G]]
[[Category: Tan, P]]
[[Category: Hong, L]]
[[Category: Dong, X]]
[[Category: Li, Y]]
[[Category: Wang, P]]
[[Category: Wu, H]]
[[Category: Ma, X]]