6j13 - Revision history

OCA at 09:57, 22 November 2023

OCA — Wed, 22 Nov 2023 09:57:10 GMT

←Older revision		Revision as of 09:57, 22 November 2023
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	<StructureSection load='6j13' size='340' side='right'caption='[[6j13]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6j13' size='340' side='right'caption='[[6j13]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j13]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J13 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6J13 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j13]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J13 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~thioredoxin peroxidase (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3055 CHLRE])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j13 OCA], [~~http~~://pdbe.org/6j13 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6j13 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6j13 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6j13 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j13 OCA], [https://pdbe.org/6j13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j13 RCSB], [https://www.ebi.ac.uk/pdbsum/6j13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j13 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9FE86_CHLRE Q9FE86_CHLRE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6j13" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6j13" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chlre]]
-	[[Category: Large Structures]]
-	[[Category: Charoenwattansatien, R]]
-	[[Category: Hippler, M]]
-	[[Category: Kurisu, G]]
-	[[Category: Tanaka, H]]
-	[[Category: Zinzius, K]]
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Peroxiredoxin~~]]	+	[[Category: Charoenwattansatien R]]
-	[[Category: ~~Redox-~~]]	+	[[Category: Hippler M]]
		+	[[Category: Kurisu G]]
		+	[[Category: Tanaka H]]
		+	[[Category: Zinzius K]]

OCA at 09:29, 1 April 2020

OCA — Wed, 01 Apr 2020 09:29:07 GMT

←Older revision		Revision as of 09:29, 1 April 2020
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	<StructureSection load='6j13' size='340' side='right'caption='[[6j13]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6j13' size='340' side='right'caption='[[6j13]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j13]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J13 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j13]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J13 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j13 OCA], [http://pdbe.org/6j13 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j13 RCSB], [http://www.ebi.ac.uk/pdbsum/6j13 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j13 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thioredoxin peroxidase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j13 OCA], [http://pdbe.org/6j13 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j13 RCSB], [http://www.ebi.ac.uk/pdbsum/6j13 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j13 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium (Ca(2+)) and redox signaling enable cells to quickly adapt to changing environments. The signaling protein calredoxin (CRX) from the green alga Chlamydomonas reinhardtii is a chloroplast-resident thioredoxin having Ca(2+)-dependent activity and harboring a unique combination of an EF-hand domain connected to a typical thioredoxin-fold. Using small-angle X-ray scattering (SAXS), FRET, and NMR techniques, we found that Ca(2+)-binding not only induces a conformational change in the EF-hand domain, but also in the thioredoxin domain, translating into the onset of thioredoxin redox activity. Functional analyses of CRX with genetically altered EF-hands revealed that EF-hand 4 is important for mediating the communication between the two domains. Moreover, we crystallized a variant (C174S) of the CRX target protein peroxiredoxin 1 (PRX1) at 2.4 A resolution, modeled the interaction complex of the two proteins, and analyzed it by cross-linking and MS analyses, revealing that the interaction interface is located close to the active sites of both proteins. Our findings shed light on the Ca(2+) binding-induced changes in CRX structure in solution at the level of the overall protein and individual domains and residues.
		+
		+	Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga Chlamydomonas reinhardtii.,Charoenwattanasatien R, Zinzius K, Scholz M, Wicke S, Tanaka H, Brandenburg JS, Marchetti GM, Ikegami T, Matsumoto T, Oda T, Sato M, Hippler M, Kurisu G J Biol Chem. 2020 Jan 3;295(1):170-180. doi: 10.1074/jbc.RA119.008735. Epub 2019 , Nov 27. PMID:31776187<ref>PMID:31776187</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6j13" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlre]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Charoenwattansatien, R]]		[[Category: Charoenwattansatien, R]]

OCA at 08:00, 4 December 2019

OCA — Wed, 04 Dec 2019 08:00:17 GMT

←Older revision		Revision as of 08:00, 4 December 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6j13 ~~is ON HOLD until Paper Publication~~	+	==Redox protein from Chlamydomonas reinhardtii==
-		+	<StructureSection load='6j13' size='340' side='right'caption='[[6j13]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~Authors~~: ~~Charoenwattansatien~~, R., ~~Zinzius, K~~., ~~Tanaka, H~~., ~~Hippler, M~~., ~~Kurisu, G~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6j13]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J13 FirstGlance]. <br>
-	~~Description: Redox protein from Chlamydomonas reinhardtii~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j13 OCA], [http://pdbe.org/6j13 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j13 RCSB], [http://www.ebi.ac.uk/pdbsum/6j13 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j13 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Charoenwattansatien, R]]
		+	[[Category: Hippler, M]]
		+	[[Category: Kurisu, G]]
	[[Category: Tanaka, H]]		[[Category: Tanaka, H]]
	[[Category: Zinzius, K]]		[[Category: Zinzius, K]]
-	[[Category: ~~Kurisu, G~~]]	+	[[Category: Chlamydomonas reinhardtii]]
-	[[Category: ~~Charoenwattansatien, R~~]]	+	[[Category: Oxidoreductase]]
-	[[Category: ~~Hippler, M~~]]	+	[[Category: Peroxiredoxin]]
		+	[[Category: Redox-]]

OCA at 13:29, 27 February 2019

OCA — Wed, 27 Feb 2019 13:29:42 GMT

←Older revision		Revision as of 13:29, 27 February 2019
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	Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G.		Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G.

-	Description: Redox protein from Chlamydomonas ~~rainhardtii~~	+	Description: Redox protein from Chlamydomonas reinhardtii
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Tanaka, H]]		[[Category: Tanaka, H]]
		+	[[Category: Zinzius, K]]
	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]
	[[Category: Charoenwattansatien, R]]		[[Category: Charoenwattansatien, R]]
	[[Category: Hippler, M]]		[[Category: Hippler, M]]
-	[[Category: Zinzius, K]]

OCA at 06:04, 30 January 2019

OCA — Wed, 30 Jan 2019 06:04:34 GMT

←Older revision		Revision as of 06:04, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6j13 is ON HOLD	+	The entry 6j13 is ON HOLD until Paper Publication

	Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G.		Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Tanaka, H]]		[[Category: Tanaka, H]]
-	[[Category: Zinzius, K]]
	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]
	[[Category: Charoenwattansatien, R]]		[[Category: Charoenwattansatien, R]]
	[[Category: Hippler, M]]		[[Category: Hippler, M]]
		+	[[Category: Zinzius, K]]

OCA: Protected "6j13" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2019 04:51:53 GMT

Protected "6j13" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:51, 2 January 2019

OCA: New page: '''Unreleased structure''' The entry 6j13 is ON HOLD Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G. Description: Redox protein from Chlamydomonas ra...

OCA — Wed, 02 Jan 2019 04:51:52 GMT

New page: '''Unreleased structure''' The entry 6j13 is ON HOLD Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G. Description: Redox protein from Chlamydomonas ra...

New page

'''Unreleased structure'''

The entry 6j13 is ON HOLD

Authors: Charoenwattansatien, R., Zinzius, K., Tanaka, H., Hippler, M., Kurisu, G.

Description: Redox protein from Chlamydomonas rainhardtii
[[Category: Unreleased Structures]]
[[Category: Tanaka, H]]
[[Category: Zinzius, K]]
[[Category: Kurisu, G]]
[[Category: Charoenwattansatien, R]]
[[Category: Hippler, M]]