6j17 - Revision history

OCA at 09:57, 22 November 2023

OCA — Wed, 22 Nov 2023 09:57:18 GMT

←Older revision		Revision as of 09:57, 22 November 2023
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	<StructureSection load='6j17' size='340' side='right'caption='[[6j17]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='6j17' size='340' side='right'caption='[[6j17]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j17]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J17 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6J17 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j17]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J17 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ATP:ADENOSINE~~-~~5-TRIPHOSPHATE'>ATP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.975Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~eccC3, Rv0284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id~~=~~83332 MYCTU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6j17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j17 OCA], [~~http~~://pdbe.org/6j17 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6j17 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6j17 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6j17 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j17 OCA], [https://pdbe.org/6j17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j17 RCSB], [https://www.ebi.ac.uk/pdbsum/6j17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j17 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ECCC3_MYCTU ECCC3_MYCTU]] Part of the ESX-3 specialized secretion system, which is important for iron and zinc uptake or homeostasis.<ref>PMID:19684129</ref> <ref>PMID:24155985</ref>	+	[https://www.uniprot.org/uniprot/ECCC3_MYCTU ECCC3_MYCTU] Part of the ESX-3 specialized secretion system, which is important for iron and zinc uptake or homeostasis.<ref>PMID:19684129</ref> <ref>PMID:24155985</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Li, J]]	+	[[Category: Li J]]
-	[[Category: Rao~~, Z H~~]]	+	[[Category: Rao ZH]]
-	[[Category: Wang~~, S H]]~~	+	[[Category: Wang SH]]
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Motor protein~~]]	+

OCA at 16:07, 29 January 2020

OCA — Wed, 29 Jan 2020 16:07:47 GMT

←Older revision		Revision as of 16:07, 29 January 2020
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	<StructureSection load='6j17' size='340' side='right'caption='[[6j17]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='6j17' size='340' side='right'caption='[[6j17]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j17]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J17 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j17]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J17 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eccC3, Rv0284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j17 OCA], [http://pdbe.org/6j17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j17 RCSB], [http://www.ebi.ac.uk/pdbsum/6j17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j17 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j17 OCA], [http://pdbe.org/6j17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j17 RCSB], [http://www.ebi.ac.uk/pdbsum/6j17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j17 ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Li, J]]		[[Category: Li, J]]
	[[Category: Rao, Z H]]		[[Category: Rao, Z H]]

OCA at 07:59, 4 December 2019

OCA — Wed, 04 Dec 2019 07:59:41 GMT

←Older revision		Revision as of 07:59, 4 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6j17 is ~~ON HOLD~~ ~~until Dec 28 2020~~	+	==ATPase==
		+	<StructureSection load='6j17' size='340' side='right'caption='[[6j17]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6j17]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J17 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j17 OCA], [http://pdbe.org/6j17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j17 RCSB], [http://www.ebi.ac.uk/pdbsum/6j17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j17 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ECCC3_MYCTU ECCC3_MYCTU]] Part of the ESX-3 specialized secretion system, which is important for iron and zinc uptake or homeostasis.<ref>PMID:19684129</ref> <ref>PMID:24155985</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence factors into host macrophages during infection. However, little is known about the molecular details as to how this occurs. Here, we provide high-resolution crystal structures of the C-terminal ATPase3 domains of EccC subunits from four different Mtb T7SS subtypes. These structures adopt a classic RecA-like a/beta fold with a conserved Mg-ATP binding site. The structure of EccCb1 in complex with the C-terminal peptide of EsxB identifies the location of substrate recognition site and shows how the specific signaling module "LxxxMxF" for Mtb ESX-1 binds to this site resulting in a translation of the bulge loop. A comparison of all the ATPase3 structures shows there are significant differences in the shape and composition of the signal recognition pockets across the family, suggesting that distinct signaling sequences of substrates are required to be specifically recognized by different T7SSs. A hexameric model of the EccC-ATPase3 is proposed and shows the recognition pocket is located near the central substrate translocation channel. The diameter of the channel is ~25-A, with a size that would allow helix-bundle shaped substrate proteins to bind and pass through. Thus, our work provides new molecular insights into substrate recognition for Mtb T7SS subtypes and also a possible transportation mechanism for substrate and/or virulence factor secretion.

-	~~Authors: Wang~~, S.H., Li, J., Rao, Z.H.	+	Structural insights into substrate recognition by the type VII secretion system.,Wang S, Zhou K, Yang X, Zhang B, Zhao Y, Xiao Y, Yang X, Yang H, Guddat LW, Li J, Rao Z Protein Cell. 2019 Nov 23. pii: 10.1007/s13238-019-00671-z. doi:, 10.1007/s13238-019-00671-z. PMID:31758528<ref>PMID:31758528</ref>

-	~~Description: ATPase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Rao, Z.H~~]]	+	<div class="pdbe-citations 6j17" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Li, J]]		[[Category: Li, J]]
-	[[Category: Wang, S.H]]	+	[[Category: Rao, Z H]]
		+	[[Category: Wang, S H]]
		+	[[Category: Atpase]]
		+	[[Category: Motor protein]]

OCA at 05:22, 6 March 2019

OCA — Wed, 06 Mar 2019 05:22:39 GMT

←Older revision		Revision as of 05:22, 6 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6j17 is ON HOLD	+	The entry 6j17 is ON HOLD until Dec 28 2020

	Authors: Wang, S.H., Li, J., Rao, Z.H.		Authors: Wang, S.H., Li, J., Rao, Z.H.

OCA: Protected "6j17" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2019 04:52:14 GMT

Protected "6j17" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:52, 2 January 2019

OCA: New page: '''Unreleased structure''' The entry 6j17 is ON HOLD Authors: Wang, S.H., Li, J., Rao, Z.H. Description: ATPase Category: Unreleased Structures Category: Rao, Z.H [[Category: L...

OCA — Wed, 02 Jan 2019 04:52:13 GMT

New page: '''Unreleased structure''' The entry 6j17 is ON HOLD Authors: Wang, S.H., Li, J., Rao, Z.H. Description: ATPase Category: Unreleased Structures Category: Rao, Z.H [[Category: L...

New page

'''Unreleased structure'''

The entry 6j17 is ON HOLD

Authors: Wang, S.H., Li, J., Rao, Z.H.

Description: ATPase
[[Category: Unreleased Structures]]
[[Category: Rao, Z.H]]
[[Category: Li, J]]
[[Category: Wang, S.H]]