6j95 - Revision history

OCA at 10:04, 22 November 2023

OCA — Wed, 22 Nov 2023 10:04:07 GMT

←Older revision		Revision as of 10:04, 22 November 2023
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	<StructureSection load='6j95' size='340' side='right'caption='[[6j95]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6j95' size='340' side='right'caption='[[6j95]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j95]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J95 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6J95 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J95 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=RET:RETINAL'>RET</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.002Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CYP97A3, LUT5, At1g31800, F5M6.19 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=3702 ARATH])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j95 OCA], [~~http~~://pdbe.org/6j95 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6j95 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6j95 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6j95 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j95 OCA], [https://pdbe.org/6j95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j95 RCSB], [https://www.ebi.ac.uk/pdbsum/6j95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j95 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LUT5_ARATH LUT5_ARATH]] Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylation of alpha- and beta-carotene. Has also a low activity toward the epsilon-rings of alpha-carotene. The beta-ring of alpha-carotene is the preferred substrate in planta.<ref>PMID:16492736</ref> <ref>PMID:16890225</ref> <ref>PMID:19147649</ref>	+	[https://www.uniprot.org/uniprot/LUT5_ARATH LUT5_ARATH] Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylation of alpha- and beta-carotene. Has also a low activity toward the epsilon-rings of alpha-carotene. The beta-ring of alpha-carotene is the preferred substrate in planta.<ref>PMID:16492736</ref> <ref>PMID:16890225</ref> <ref>PMID:19147649</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guo, Q]]	+	[[Category: Guo Q]]
-	[[Category: Niu, G]]	+	[[Category: Niu G]]
-	[[Category: Wang, J]]	+	[[Category: Wang J]]
-	[[Category: Zhao, S]]	+	[[Category: Zhao S]]
-	~~[[Category: Carotenoid]]~~	+
-	~~[[Category: Lutein biosynthesis]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: P450]]~~	+
-	~~[[Category: Photosynthesis~~]]	+

OCA at 06:48, 25 June 2020

OCA — Thu, 25 Jun 2020 06:48:35 GMT

←Older revision		Revision as of 06:48, 25 June 2020
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LUT5_ARATH LUT5_ARATH]] Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylation of alpha- and beta-carotene. Has also a low activity toward the epsilon-rings of alpha-carotene. The beta-ring of alpha-carotene is the preferred substrate in planta.<ref>PMID:16492736</ref> <ref>PMID:16890225</ref> <ref>PMID:19147649</ref>		[[http://www.uniprot.org/uniprot/LUT5_ARATH LUT5_ARATH]] Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylation of alpha- and beta-carotene. Has also a low activity toward the epsilon-rings of alpha-carotene. The beta-ring of alpha-carotene is the preferred substrate in planta.<ref>PMID:16492736</ref> <ref>PMID:16890225</ref> <ref>PMID:19147649</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two cytochrome P450 enzymes, CYP97A3 and CYP97C1, catalyze hydroxylations of the beta- and epsilon-rings of alpha-carotene to produce lutein. Chirality is introduced at the C-3 atom of both rings, and the reactions are both pro-3R-stereospecific. We determined the crystal structures of CYP97A3 in substrate-free and complex forms with a nonnatural substrate and the structure of CYP97C1 in a detergent-bound form. The structures of CYP97A3 in different states show the substrate channel and the structure of CYP97C1 bound with octylthioglucoside confirms the binding site for the carotenoid substrate. Biochemical assays confirm that the ferredoxin-NADP(+) reductase (FNR)-ferredoxin pair is used as the redox partner. Details of the pro-3R stereospecificity are revealed in the retinal-bound CYP97A3 structure. Further analysis indicates that the CYP97B clan bears similarity to the beta-ring-specific CYP97A clan. Overall, our research describes the molecular basis for the last steps of lutein biosynthesis.
		+
		+	Structural basis for plant lutein biosynthesis from alpha-carotene.,Niu G, Guo Q, Wang J, Zhao S, He Y, Liu L Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14150-14157. doi:, 10.1073/pnas.2001806117. Epub 2020 Jun 8. PMID:32513704<ref>PMID:32513704</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6j95" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:34, 10 June 2020

OCA — Wed, 10 Jun 2020 06:34:00 GMT

←Older revision		Revision as of 06:34, 10 June 2020
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	<StructureSection load='6j95' size='340' side='right'caption='[[6j95]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6j95' size='340' side='right'caption='[[6j95]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j95]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J95 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6J95 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j95]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J95 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J95 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j95 OCA], [http://pdbe.org/6j95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j95 RCSB], [http://www.ebi.ac.uk/pdbsum/6j95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j95 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYP97A3, LUT5, At1g31800, F5M6.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j95 OCA], [http://pdbe.org/6j95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j95 RCSB], [http://www.ebi.ac.uk/pdbsum/6j95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j95 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Guo, Q]]		[[Category: Guo, Q]]

OCA at 16:08, 22 January 2020

OCA — Wed, 22 Jan 2020 16:08:02 GMT

←Older revision		Revision as of 16:08, 22 January 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6j95 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of CYP97A3 in complex with retinal==
-		+	<StructureSection load='6j95' size='340' side='right'caption='[[6j95]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Niu~~, G., ~~Guo~~, Q.~~, Wang, J~~., ~~Zhao~~, S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6j95]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J95 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J95 FirstGlance]. <br>
-	~~Description: Crystal structure of CYP97A3 in complex with retinal~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j95 OCA], [http://pdbe.org/6j95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j95 RCSB], [http://www.ebi.ac.uk/pdbsum/6j95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j95 ProSAT]</span></td></tr>
-	[[Category: ~~Zhao, S~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LUT5_ARATH LUT5_ARATH]] Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylation of alpha- and beta-carotene. Has also a low activity toward the epsilon-rings of alpha-carotene. The beta-ring of alpha-carotene is the preferred substrate in planta.<ref>PMID:16492736</ref> <ref>PMID:16890225</ref> <ref>PMID:19147649</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Guo, Q]]		[[Category: Guo, Q]]
		+	[[Category: Niu, G]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
-	[[Category: ~~Niu~~, G]]	+	[[Category: Zhao, S]]
		+	[[Category: Carotenoid]]
		+	[[Category: Lutein biosynthesis]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: P450]]
		+	[[Category: Photosynthesis]]

OCA at 07:16, 13 February 2019

OCA — Wed, 13 Feb 2019 07:16:35 GMT

←Older revision		Revision as of 07:16, 13 February 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6j95 is ON HOLD	+	The entry 6j95 is ON HOLD until Paper Publication

	Authors: Niu, G., Guo, Q., Wang, J., Zhao, S.		Authors: Niu, G., Guo, Q., Wang, J., Zhao, S.

OCA: Protected "6j95" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2019 06:16:24 GMT

Protected "6j95" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:16, 30 January 2019

OCA: New page: '''Unreleased structure''' The entry 6j95 is ON HOLD Authors: Niu, G., Guo, Q., Wang, J., Zhao, S. Description: Crystal structure of CYP97A3 in complex with retinal [[Category: Unrelea...

OCA — Wed, 30 Jan 2019 06:16:22 GMT

New page: '''Unreleased structure''' The entry 6j95 is ON HOLD Authors: Niu, G., Guo, Q., Wang, J., Zhao, S. Description: Crystal structure of CYP97A3 in complex with retinal [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 6j95 is ON HOLD

Authors: Niu, G., Guo, Q., Wang, J., Zhao, S.

Description: Crystal structure of CYP97A3 in complex with retinal
[[Category: Unreleased Structures]]
[[Category: Zhao, S]]
[[Category: Guo, Q]]
[[Category: Wang, J]]
[[Category: Niu, G]]