6jc5 - Revision history

OCA at 10:27, 15 November 2023

OCA — Wed, 15 Nov 2023 10:27:08 GMT

←Older revision		Revision as of 10:27, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='6jc5' size='340' side='right'caption='[[6jc5]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='6jc5' size='340' side='right'caption='[[6jc5]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jc5]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JC5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JC5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jc5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_haddoni Stichodactyla haddoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JC5 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=BJF:'>BJF</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.051Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[~~[6jc6\|6jc6]~~]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BJF:{2-[(1S)-1-amino-3-methylbutyl]-5-hydroxy-4-(2-methylpropyl)-1H-imidazol-1-yl}acetic+acid'>BJF</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jc5 OCA], [~~http~~://pdbe.org/6jc5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jc5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jc5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jc5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jc5 OCA], [https://pdbe.org/6jc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jc5 RCSB], [https://www.ebi.ac.uk/pdbsum/6jc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jc5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A4V8H022_STIHA A0A4V8H022_STIHA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6jc5" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6jc5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chang, H Y~~]]	+	[[Category: Stichodactyla haddoni]]
-	[[Category: ~~Huang, K F~~]]	+	[[Category: Chang HY]]
-	[[Category: ~~Ko, T P~~]]	+	[[Category: Huang KF]]
-	[[Category: ~~Biomarker]]~~	+	[[Category: Ko TP]]
-	~~[[Category: Emission spectra]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Green fluorescent protein]]~~	+
-	~~[[Category: Sea anemone]]~~	+
-	~~[[Category: Uv absorption~~]]	+

OCA at 08:00, 20 March 2019

OCA — Wed, 20 Mar 2019 08:00:44 GMT

←Older revision		Revision as of 08:00, 20 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jc5 is ~~ON HOLD until Jan 28 2021~~	+	==Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni==
		+	<StructureSection load='6jc5' size='340' side='right'caption='[[6jc5]], [[Resolution\|resolution]] 2.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jc5]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JC5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JC5 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BJF:'>BJF</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6jc6\|6jc6]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jc5 OCA], [http://pdbe.org/6jc5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jc5 RCSB], [http://www.ebi.ac.uk/pdbsum/6jc5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jc5 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chromoproteins are a good source of engineered biological tools. We previously reported the development of a blue fluorescent protein, termed shBFP, which was derived from a purple chromoprotein shCP found in the sea anemone Stichodacyla haddoni. shBFP contains a Leu(63)-Leu(64)-Gly(65) tri-peptide chromophore, and shows maximum excitation and emission wavelengths at 401nm and 458nm, along with a high quantum yield. How this chromophore endows shBFP with the unique fluorescence property in the absence of a hydroxyphenyl ring remained unclear. Here, we present the crystal structures of shCP and shBFP at 1.9- and 2.05-A resolution, respectively. Both proteins crystallized as similar tetramers, but they are more likely to function as dimers in solution. The chromophore in shCP shows a trans-conformation and its non-planarity is similar to most other homologues. The shBFP chromophore also contains an imidazolidone moiety in its structure, but there are a smaller number of conjugated double bonds compared to shCP. Consequently, the chromophore may prefer absorbing shorter wavelength lights in the UV region, followed by the emission of blue fluorescence. These observations provide new insights into the molecular basis that correlates chromophore conformation with light absorption and fluorescence emission for the development of improved biomarkers.

-	~~Authors:~~ Ko, ~~T.P.~~, Huang, ~~K.F.~~, ~~Chang~~, H.Y.	+	Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni.,Chang HY, Ko TP, Chang YC, Huang KF, Lin CY, Chou HY, Chiang CY, Tsai HJ Int J Biol Macromol. 2019 Mar 2;130:675-684. doi: 10.1016/j.ijbiomac.2019.02.138. PMID:30836182<ref>PMID:30836182</ref>

-	~~Description: Crystal structure~~ of the ~~blue fluorescent protein with a Leu~~-~~Leu~~-~~Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Huang, K.F]]	+	<div class="pdbe-citations 6jc5" style="background-color:#fffaf0;"></div>
-	[[Category: Ko, T.P]]	+	== References ==
-	[[Category: ~~Chang, H.Y~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang, H Y]]
		+	[[Category: Huang, K F]]
		+	[[Category: Ko, T P]]
		+	[[Category: Biomarker]]
		+	[[Category: Emission spectra]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Green fluorescent protein]]
		+	[[Category: Sea anemone]]
		+	[[Category: Uv absorption]]

OCA at 04:57, 21 February 2019

OCA — Thu, 21 Feb 2019 04:57:28 GMT

←Older revision		Revision as of 04:57, 21 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jc5 is ON HOLD	+	The entry 6jc5 is ON HOLD until Jan 28 2021

	Authors: Ko, T.P., Huang, K.F., Chang, H.Y.		Authors: Ko, T.P., Huang, K.F., Chang, H.Y.
Line 7:		Line 7:
	Description: Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni		Description: Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Huang, K.F]]
	[[Category: Ko, T.P]]		[[Category: Ko, T.P]]
	[[Category: Chang, H.Y]]		[[Category: Chang, H.Y]]
-	[[Category: Huang, K.F]]

OCA: Protected "6jc5" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2019 07:20:59 GMT

Protected "6jc5" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:20, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6jc5 is ON HOLD Authors: Ko, T.P., Huang, K.F., Chang, H.Y. Description: Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-p...

OCA — Wed, 13 Feb 2019 07:20:58 GMT

New page: '''Unreleased structure''' The entry 6jc5 is ON HOLD Authors: Ko, T.P., Huang, K.F., Chang, H.Y. Description: Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-p...

New page

'''Unreleased structure'''

The entry 6jc5 is ON HOLD

Authors: Ko, T.P., Huang, K.F., Chang, H.Y.

Description: Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni
[[Category: Unreleased Structures]]
[[Category: Ko, T.P]]
[[Category: Chang, H.Y]]
[[Category: Huang, K.F]]