6jgx - Revision history

OCA at 10:08, 22 November 2023

OCA — Wed, 22 Nov 2023 10:08:53 GMT

←Older revision		Revision as of 10:08, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6jgx' size='340' side='right'caption='[[6jgx]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='6jgx' size='340' side='right'caption='[[6jgx]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jgx]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JGX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JGX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jgx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JGX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CD:CADMIUM+ION'>CD</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.71Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>cadR, BIW19_10095, BL240_26950 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~303~~ "~~Bacillus fluorescens putidus~~" ~~Flugge 1886])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jgx OCA], [~~http~~://pdbe.org/6jgx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jgx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jgx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jgx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jgx OCA], [https://pdbe.org/6jgx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jgx RCSB], [https://www.ebi.ac.uk/pdbsum/6jgx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jgx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93TP7_PSEPU Q93TP7_PSEPU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chen, H~~]]	+	[[Category: Pseudomonas putida]]
-	[[Category: ~~Gan, J~~ H]]	+	[[Category: Chen H]]
-	[[Category: ~~Liu, X C~~]]	+	[[Category: Gan JH]]
-	[[Category: ~~Cadmium regulator]]~~	+	[[Category: Liu XC]]
-	~~[[Category: Cadr]]~~	+
-	~~[[Category: Merr family]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription-dna complex~~]]	+

OCA at 06:07, 10 October 2019

OCA — Thu, 10 Oct 2019 06:07:05 GMT

←Older revision		Revision as of 06:07, 10 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jgx is ~~ON HOLD~~	+	==Crystal structure of the transcriptional regulator CadR from P. putida in complex with Cadmium(II) and DNA==
		+	<StructureSection load='6jgx' size='340' side='right'caption='[[6jgx]], [[Resolution\|resolution]] 2.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jgx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JGX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JGX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cadR, BIW19_10095, BL240_26950 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jgx OCA], [http://pdbe.org/6jgx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jgx RCSB], [http://www.ebi.ac.uk/pdbsum/6jgx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jgx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Detoxification of the highly toxic cadmium element is essential for the survival of living organisms. Pseudomonas putida CadR, a MerR family transcriptional regulator, has been reported to exhibit an ultraspecific response to the cadmium ion. Our crystallographic and spectroscopic studies reveal that the extra cadmium selectivity of CadR is mediated by the unexpected cooperation of thiolate-rich site I and histidine-rich site II. Cadmium binding in site I mediates the reorientation of protein domains and facilitates the assembly of site II. Subsequently, site II bridge-links 2 DNA binding domains through ligands His140/His145 in the C-terminal histidine-rich tail. With dynamic transit between 2 conformational states, this bridge could stabilize the regulator into an optimal conformation that is critical for enhancing the transcriptional activity of the cadmium detoxification system. Our results provide dynamic insight into how nature utilizes the unique cooperative binding mechanism in multisite proteins to recognize cadmium ions specifically.

-	~~Authors: Liu~~, X~~.C.~~, ~~Gan~~, J~~.H.~~, Chen, H.	+	Selective cadmium regulation mediated by a cooperative binding mechanism in CadR.,Liu X, Hu Q, Yang J, Huang S, Wei T, Chen W, He Y, Wang D, Liu Z, Wang K, Gan J, Chen H Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20398-20403. doi:, 10.1073/pnas.1908610116. Epub 2019 Sep 23. PMID:31548408<ref>PMID:31548408</ref>

-	~~Description: Crystal structure~~ of the ~~transcriptional regulator CadR from P~~. ~~putida in complex with Cadmium(II) and DNA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Gan, J.H~~]]	+	<div class="pdbe-citations 6jgx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus fluorescens putidus flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
-	[[Category: Liu, X.C]]	+	[[Category: Gan, J H]]
		+	[[Category: Liu, X C]]
		+	[[Category: Cadmium regulator]]
		+	[[Category: Cadr]]
		+	[[Category: Merr family]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription-dna complex]]

OCA: Protected "6jgx" [edit=sysop:move=sysop]

OCA — Thu, 21 Feb 2019 05:01:38 GMT

Protected "6jgx" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:01, 21 February 2019

OCA: New page: '''Unreleased structure''' The entry 6jgx is ON HOLD Authors: Liu, X.C., Gan, J.H., Chen, H. Description: Crystal structure of the transcriptional regulator CadR from P. putida in comp...

OCA — Thu, 21 Feb 2019 05:01:37 GMT

New page: '''Unreleased structure''' The entry 6jgx is ON HOLD Authors: Liu, X.C., Gan, J.H., Chen, H. Description: Crystal structure of the transcriptional regulator CadR from P. putida in comp...

New page

'''Unreleased structure'''

The entry 6jgx is ON HOLD

Authors: Liu, X.C., Gan, J.H., Chen, H.

Description: Crystal structure of the transcriptional regulator CadR from P. putida in complex with Cadmium(II) and DNA
[[Category: Unreleased Structures]]
[[Category: Gan, J.H]]
[[Category: Chen, H]]
[[Category: Liu, X.C]]