6jh7 - Revision history

OCA at 10:08, 22 November 2023

OCA — Wed, 22 Nov 2023 10:08:59 GMT

←Older revision		Revision as of 10:08, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6jh7' size='340' side='right'caption='[[6jh7]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='6jh7' size='340' side='right'caption='[[6jh7]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jh7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JH7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_FACHB-905_=_DIANCHI905 Microcystis aeruginosa FACHB-905 = DIANCHI905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JH7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.38Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jh7 OCA], [~~http~~://pdbe.org/6jh7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jh7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jh7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jh7 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jh7 OCA], [https://pdbe.org/6jh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jh7 RCSB], [https://www.ebi.ac.uk/pdbsum/6jh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jh7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Qiu, X~~]]	+	[[Category: Microcystis aeruginosa FACHB-905 = DIANCHI905]]
-	[[Category: ~~Biosynthetic protein]]~~	+	[[Category: Qiu X]]
-	~~[[Category: Reductase~~]]	+

OCA at 06:46, 27 November 2019

OCA — Wed, 27 Nov 2019 06:46:53 GMT

←Older revision		Revision as of 06:46, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jh7 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of AerF from Microcystis aeruginosa==
		+	<StructureSection load='6jh7' size='340' side='right'caption='[[6jh7]], [[Resolution\|resolution]] 1.38Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jh7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JH7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jh7 OCA], [http://pdbe.org/6jh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jh7 RCSB], [http://www.ebi.ac.uk/pdbsum/6jh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jh7 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 2-carboxy-6-hydroxyoctahydroindole (Choi) moiety is an essential residue for the antithrombotic activities of aeruginosins, which are a class of cyanobacterial derived bioactive linear tetrapeptides. Biosynthetic pathway of Choi is still elusive. AerF was suggested to be involved in the biosynthesis of Choi, and can be assigned to the short-chain dehydrogenase/reductase (SDR) superfamily. However, both the exact role and the catalytic mechanism of AerF have not been elucidated. In this study, functional and mechanistic analyses of AerF from Microcystis aeruginosa were performed. Observation of enzymatic assay demonstrates that AerF is a NADPH-dependent alkenal double bond reductase that catalyzes the reduction of dihydro-4-hydroxyphenylpyruvate (H2HPP) to generate tetrahydro-4-hydroxyphenylpyruvate (H4HPP), which is the third step of the biosynthetic pathway from prephenate to Choi. Comparative structural analysis indicates that ligand binding-induced conformational change of AerF is different from that of the other SDR superfamily reductase using H2HPP as a substrate. Analyses of NADPH and substrate analogue binding sites combined with the results of mutagenesis analyses suggest that a particular serine residue mainly involves in the initiation of the proton transfer between the substrate and the residues of AerF, which is an uncommon feature in SDR superfamily reductase. Furthermore, based on the observations of structural and mutagenesis analyses, the catalytic mechanism of AerF is proposed and a proton transfer pathway in AerF is deduced.

-	~~Authors:~~ Qiu, X.	+	Structural and functional investigation of AerF, a NADPH-dependent alkenal double bond reductase participating in the biosynthesis of Choi moiety of aeruginosin.,Qiu X, Wei Y, Zhu W, Fu J, Duan X, Jin H, Zhu P, Zhou C, Yan X J Struct Biol. 2019 Nov 11:107415. doi: 10.1016/j.jsb.2019.107415. PMID:31726097<ref>PMID:31726097</ref>

-	~~Description: Crystal structure~~ of ~~AerF from Microcystis aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6jh7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Qiu, X]]		[[Category: Qiu, X]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Reductase]]

OCA at 05:26, 6 March 2019

OCA — Wed, 06 Mar 2019 05:26:08 GMT

←Older revision		Revision as of 05:26, 6 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jh7 is ON HOLD	+	The entry 6jh7 is ON HOLD until Paper Publication

	Authors: Qiu, X.		Authors: Qiu, X.

OCA: Protected "6jh7" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2019 13:35:19 GMT

Protected "6jh7" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:35, 27 February 2019

OCA: New page: '''Unreleased structure''' The entry 6jh7 is ON HOLD Authors: Qiu, X. Description: Crystal structure of AerF from Microcystis aeruginosa Category: Unreleased Structures [[Category:...

OCA — Wed, 27 Feb 2019 13:35:17 GMT

New page: '''Unreleased structure''' The entry 6jh7 is ON HOLD Authors: Qiu, X. Description: Crystal structure of AerF from Microcystis aeruginosa Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 6jh7 is ON HOLD

Authors: Qiu, X.

Description: Crystal structure of AerF from Microcystis aeruginosa
[[Category: Unreleased Structures]]
[[Category: Qiu, X]]