6jrg - Revision history

OCA at 10:18, 22 November 2023

OCA — Wed, 22 Nov 2023 10:18:50 GMT

←Older revision		Revision as of 10:18, 22 November 2023
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	<StructureSection load='6jrg' size='340' side='right'caption='[[6jrg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6jrg' size='340' side='right'caption='[[6jrg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jrg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JRG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jrg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JRG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.005Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Moc1, 100192759, ZEAMMB73_Zm00001d040409 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrg OCA], [~~http~~://pdbe.org/6jrg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jrg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jrg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrg OCA], [https://pdbe.org/6jrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jrg RCSB], [https://www.ebi.ac.uk/pdbsum/6jrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B4FCI7_MAIZE B4FCI7_MAIZE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Maize~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Lin, H]]	+	[[Category: Zea mays]]
-	[[Category: Lin, Z]]	+	[[Category: Lin H]]
-	[[Category: Yuan, C]]	+	[[Category: Lin Z]]
-	[[Category: Zhang, D]]	+	[[Category: Yuan C]]
-	~~[[Category: Dna binding protein]]~~	+	[[Category: Zhang D]]
-	~~[[Category: Dna binding protein-dna complex]]~~	+
-	~~[[Category: Holliday junction resolvase-dna complex~~]]	+

OCA at 09:33, 30 October 2019

OCA — Wed, 30 Oct 2019 09:33:37 GMT

←Older revision		Revision as of 09:33, 30 October 2019
Line 3:		Line 3:
	<StructureSection load='6jrg' size='340' side='right'caption='[[6jrg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6jrg' size='340' side='right'caption='[[6jrg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jrg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JRG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jrg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JRG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Moc1, 100192759, ZEAMMB73_Zm00001d040409 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrg OCA], [http://pdbe.org/6jrg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jrg RCSB], [http://www.ebi.ac.uk/pdbsum/6jrg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrg OCA], [http://pdbe.org/6jrg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jrg RCSB], [http://www.ebi.ac.uk/pdbsum/6jrg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrg ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Holliday junction (HJ) is a key intermediate during homologous recombination and DNA double-strand break repair. Timely HJ resolution by resolvases is critical for maintaining genome stability. The mechanisms underlying sequence-specific substrate recognition and cleavage by resolvases remain elusive. The monokaryotic chloroplast 1 protein (MOC1) specifically cleaves four-way DNA junctions in a sequence-specific manner. Here, we report the crystal structures of MOC1 from Zea mays, alone or bound to HJ DNA. MOC1 uses a unique beta-hairpin to embrace the DNA junction. A base-recognition motif specifically interacts with the junction center, inducing base flipping and pseudobase-pair formation at the strand-exchanging points. Structures of MOC1 bound to HJ and different metal ions support a two-metal ion catalysis mechanism. Further molecular dynamics simulations and biochemical analyses reveal a communication between specific substrate recognition and metal ion-dependent catalysis. Our study thus provides a mechanism for how a resolvase turns substrate specificity into catalytic efficiency.
		+
		+	Structural basis of sequence-specific Holliday junction cleavage by MOC1.,Lin H, Zhang D, Zuo K, Yuan C, Li J, Huang M, Lin Z Nat Chem Biol. 2019 Oct 14. pii: 10.1038/s41589-019-0377-4. doi:, 10.1038/s41589-019-0377-4. PMID:31611704<ref>PMID:31611704</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6jrg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Maize]]
	[[Category: Lin, H]]		[[Category: Lin, H]]
	[[Category: Lin, Z]]		[[Category: Lin, Z]]

OCA at 06:49, 23 October 2019

OCA — Wed, 23 Oct 2019 06:49:11 GMT

←Older revision		Revision as of 06:49, 23 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jrg ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of ZmMoc1 H253A mutant in complex with Holliday junction==
-		+	<StructureSection load='6jrg' size='340' side='right'caption='[[6jrg]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6jrg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JRG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrg OCA], [http://pdbe.org/6jrg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jrg RCSB], [http://www.ebi.ac.uk/pdbsum/6jrg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrg ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lin, H]]
		+	[[Category: Lin, Z]]
		+	[[Category: Yuan, C]]
		+	[[Category: Zhang, D]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna binding protein-dna complex]]
		+	[[Category: Holliday junction resolvase-dna complex]]

OCA at 03:58, 17 April 2019

OCA — Wed, 17 Apr 2019 03:58:05 GMT

←Older revision		Revision as of 03:58, 17 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jrg is ON HOLD	+	The entry 6jrg is ON HOLD until Paper Publication

-	Authors: ~~Lin, Z., Lin, H., Zhang, D., Yuan, C.~~	+	Authors:

-	Description: ~~Crystal structure of ZmMoc1-Holliday junction Complex in the presence of Calcium~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Lin, H]]
-	[[Category: Zhang, D]]
-	[[Category: Yuan, C]]
-	[[Category: Lin, Z]]

OCA: Protected "6jrg" [edit=sysop:move=sysop]

OCA — Wed, 10 Apr 2019 05:25:20 GMT

Protected "6jrg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 10 April 2019

OCA: New page: '''Unreleased structure''' The entry 6jrg is ON HOLD Authors: Lin, Z., Lin, H., Zhang, D., Yuan, C. Description: Crystal structure of ZmMoc1-Holliday junction Complex in the presence o...

OCA — Wed, 10 Apr 2019 05:25:18 GMT

New page: '''Unreleased structure''' The entry 6jrg is ON HOLD Authors: Lin, Z., Lin, H., Zhang, D., Yuan, C. Description: Crystal structure of ZmMoc1-Holliday junction Complex in the presence o...

New page

'''Unreleased structure'''

The entry 6jrg is ON HOLD

Authors: Lin, Z., Lin, H., Zhang, D., Yuan, C.

Description: Crystal structure of ZmMoc1-Holliday junction Complex in the presence of Calcium
[[Category: Unreleased Structures]]
[[Category: Lin, H]]
[[Category: Zhang, D]]
[[Category: Yuan, C]]
[[Category: Lin, Z]]